Nano black selenium peroxide analogue enzyme and preparation method thereof

A technology of black selenium peroxide and peroxidase, which is applied in chemical instruments and methods, physical/chemical process catalysts, elemental selenium/tellurium, etc., can solve problems such as difficult purification, easy inactivation of natural enzymes, and high price. Achieve the effect of easy separation and purification, simple preparation method and low price

Active Publication Date: 2015-03-25
QINGDAO UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Aiming at the problems of easy inactivation, difficult purification and high price of natural enzymes, the present invention provides a nano black selenium peroxide mimetic enzyme that is not easy to inactivate, easy to purify and low in price and a preparation method thereof

Method used

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  • Nano black selenium peroxide analogue enzyme and preparation method thereof
  • Nano black selenium peroxide analogue enzyme and preparation method thereof
  • Nano black selenium peroxide analogue enzyme and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Preparation of nanometer black selenium peroxide mimetic enzyme:

[0031] step:

[0032] Weigh 0.222g selenium dioxide (SeO 2 ), dissolved in 5 mL of deionized water at room temperature, added 0.8 mL of concentrated sulfuric acid with a mass concentration of 98%, stirred for 5 minutes, added 30 mL of glycerol, continued to stir for 30 minutes, transferred the mixture to an autoclave, and reacted at 180 ° C After 6 hours, the product was centrifuged, and the centrifuged precipitate was washed three times with deionized water and ethanol respectively, and then vacuum-dried at 60° C. to obtain nano black selenium powder.

[0033] result:

[0034] attached by figure 1 It can be seen that the prepared product is simple selenium with high purity.

[0035] attached by figure 2 It can be seen that the prepared black selenium is a spherical particle with a size between 500 nm and 10 μm.

[0036] 2. Test of the simulated activity of nano black selenium peroxidase:

[0...

Embodiment 2

[0063] Weigh 0.111g selenium dioxide (SeO 2 ), dissolved in 5 mL of deionized water at room temperature, added 0.4 mL of concentrated sulfuric acid with a mass concentration of 98%, stirred for 5 minutes, added 30 mL of glycerol, continued to stir for 30 minutes, transferred the mixture to an autoclave, and reacted at 120 ° C After 10 hours, the product was centrifuged, and the centrifuged precipitate was washed three times with deionized water and ethanol respectively, and then vacuum-dried at 60° C. to obtain nano black selenium powder.

Embodiment 3

[0065] Weigh 0.022g selenium dioxide (SeO 2 ), dissolved in 7.5mL of deionized water at room temperature, added 0.08mL of concentrated sulfuric acid with a mass concentration of 98%, stirred for 5 minutes, added 30mL of glycerol, continued to stir for 30 minutes, transferred the mixture to an autoclave, and heated at 150°C After reacting for 8 hours, the product was centrifuged, and the centrifuged precipitate was washed three times with deionized water and ethanol respectively, and vacuum-dried at 60° C. to obtain nano black selenium powder.

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Abstract

The invention discloses nano black selenium with peroxidase simulating activity, and a preparation method of the nano black selenium, and belongs to the fields of preparation and application of nano materials. The preparation method is as follows: nano-sized black elemental selenium is prepared from selenium dioxide as a raw material and concentrated sulfuric acid as a reducing agent under a solvent thermal condition. The substance has catalytic activity of similar peroxidase; and chromogenic reaction of a peroxidase substrate 3,3',5,5'-tetramethyl benzidine and hydrogen peroxide can be catalyzed, so that the preparation method is simple; the reaction product is easy to separate and purify; the used reaction apparatus and reagent are low in price; the defects that a natural enzyme is difficult to purify and high in price are overcome; and the nano black selenium can be applied to a working environment with easy occurrence of denaturation, and has a good application prospect in the field of green synthesis of a water-soluble conductive polymer.

Description

technical field [0001] The invention relates to a nano black selenium peroxide mimetic enzyme and a preparation method thereof, belonging to the technical field of nano material preparation and application. Background technique [0002] With the enhancement of human environmental protection awareness and the concept of social sustainable development, enzyme-catalyzed technology, as a green synthetic technology, has become a hot research and application hotspot in the field of chemical industry. As the core of industrial biotechnology, enzyme catalysis technology is known as the most promising technology for industrial sustainable development. Biocatalysis and biotransformation technologies will be a strong guarantee for my country's biochemical industry to realize changes in production methods, product structure adjustments, and clean and efficient manufacturing. In recent years, with the rise of green chemistry, enzyme catalysis, as an important part of green chemistry, ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J27/057C01B19/02C08G73/02C08G73/06C08G61/12
Inventor 王玮李雷雷陈克正
Owner QINGDAO UNIV OF SCI & TECH
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