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A method for ultra-high pressure extraction of cordycepin in rice culture medium of Cordyceps militaris

A culture medium and the technology of Cordyceps militaris, which is applied in the biological field, can solve the problems of limited sample volume and unsuitability for large-scale preparation of cordycepin, and achieve the effects of saving material consumption, high recovery rate and improving yield

Active Publication Date: 2016-10-26
武汉添年科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the limited amount of injection, it is not suitable for large-scale preparation of cordycepin

Method used

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  • A method for ultra-high pressure extraction of cordycepin in rice culture medium of Cordyceps militaris
  • A method for ultra-high pressure extraction of cordycepin in rice culture medium of Cordyceps militaris
  • A method for ultra-high pressure extraction of cordycepin in rice culture medium of Cordyceps militaris

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Take 30.0 g of Cordyceps militaris medium powder that has been crushed and passed through a 20-mesh sieve in a sealable plastic container, add distilled water at a material-to-liquid ratio of 1:70 g / ml, stir evenly to make it fully wet, and use a vacuum packaging machine to remove To ensure that there are no air bubbles in the container, put it into the ultra-high pressure treatment device, add the loading medium tap water into the treatment chamber, use the depth gauge to check the liquid level until the height is appropriate, remove the depth gauge and push the plunger to the pressurized position, Close the protective door of the ultra-high voltage host and prepare to start pressurization. Set the pressure to 400MPa, and hold the pressure for 15 minutes. After the treatment is completed and the pressure is relieved, take out the container, pour out the extract and centrifuge (4000r / min, 10min), and discard the residue to obtain an ultra-high pressure extract.

[0030]...

Embodiment 2

[0034] The difference from Example 1 is that the medium powder of Cordyceps militaris used was crushed through a 60-mesh sieve, and its content was finally measured to be 41.09%, and the ultrahigh-pressure extraction yield was 1558.556ug / g, and the crude cordycepin was extracted by ultra-high pressure after freeze-drying The mass is 1.311g, and the purity of the dried crude cordycepin is 94.02% after separation by countercurrent chromatography.

Embodiment 3

[0036] The difference from Example 1 is that the used Cordyceps militaris medium powder is crushed through a 60-mesh sieve, and the pressure is 600Mpa. Finally, the content of cordycepin in the ultra-high pressure extract is measured to be 40.57%, and the extraction yield is 1393.71ug / g. The mass of the crude cordycepin extracted by ultra-high pressure after drying was 1.142 g, and the purity of the dried crude cordycepin after separation by countercurrent chromatography was 91.82%.

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Abstract

The invention relates to a method for extracting cordycepin from a cordyceps militaris rice medium under ultrahigh pressure, and belongs to the technical field of natural products. The technological process of the method comprises the following steps: performing slice on the cordyceps militaris rice medium, drying with hot air, crushing, extracting under ultrahigh pressure, performing centrifugation, removing impurities through a ceramic membrane, concentrating, freeze-drying, purifying cordycepin through a high-speed countercurrent chromatography, and detecting through a high performance liquid chromatography. According the method, the yield of the cordycepin from the cordyceps militaris rice medium is 1.2 times that of a common method, and the purity can reach more than 90%. The method adopts ultrahigh pressure to extract the cordycepin, so that advantages of high extraction rate, low impurity content, good stability and the like are realized; high temperature is prevented in the whole extraction process, so that active ingredients and structures of the cordycepin can be effectively retained. The cordycepin prepared by the method is safe and free from contamination, has a high food (drug) use value, and provides a certain market application prospect and economic benefits for comprehensive utilization of edible fungus cordyceps militaris by-products.

Description

technical field [0001] The invention specifically relates to a method for rapidly and efficiently extracting cordycepin from rice medium of Cordyceps militaris, belonging to the field of biological technology. Cordycepin in Cordyceps militaris medium was extracted by ultra-high pressure, and impurities were initially removed from the extract by ceramic membrane, and high-purity cordycepin was finally prepared by high-speed countercurrent chromatography. Background technique [0002] Cordyceps militaris, as a medicinal (edible) fungus, has a similar effect to Cordyceps sinensis, and has been recognized and widely accepted by the people of our country. With the rise of market demand, the technology of artificial cultivation of Cordyceps militaris continues to mature and is widely used in the market, and while achieving good market effects and economic benefits, the problem that arises is that the residual medium after harvesting fruiting bodies is discarded in The environment...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H19/16C07H1/06
CPCC07H1/06C07H19/16
Inventor 程薇高虹陈丽冰吴光旭史德芳范秀芝郭鹏陈明利薛淑静杨德乔宇汪兰吴文锦李露
Owner 武汉添年科技有限公司
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