Method for testing group of biomarkers

A technology of biomarkers and assay methods, applied in the field of biomarker assays, can solve problems such as adverse pollutant exposure assessment, and achieve the effects of avoiding matrix interference, good repeatability, and high sensitivity

Active Publication Date: 2015-03-25
INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Abstract
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Problems solved by technology

[0011] For the detection of biomarkers, at present, most of them use a single component, different detection technologies, and separat...

Method used

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  • Method for testing group of biomarkers

Examples

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Embodiment 1

[0045] Example 1 Detection of Biomarker Content in Rabbit Meat

[0046] Now illustrate the present invention with the following implementation examples, but not limit the scope of the present invention.

[0047] Instruments and reagents used in the embodiments API4000Q type quadrupole tandem mass spectrometer distribution electrospray ion source (AB company of the United States); Agilent 1100 liquid chromatograph (Agilent company of the United States); Vortex mixer (Thermo company of the United States); sigma Low-temperature centrifuge (Sigma, USA); rotary evaporator concentrator (Heidolph, Germany); Gilson pipette (Gilson, France); nitrogen blowing instrument (Jic, USA); ultrapure water device Mili-Q (Millipone, USA) ; Octadecylsilane bonded phase (C18) purification agent (40 ~ 60μm) was purchased from Tianjin Bona Aijieer Technology Co., Ltd.

[0048] Reagents: Methanol, acetonitrile, n-hexane, ethyl acetate, formic acid, and acetic acid were all purchased from Dima Company...

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Abstract

The invention discloses a method for testing a group of biomarkers. The method comprises the following steps: (1) extraction and purification; (2) preparation of standard working solutions, wherein blank samples are processed with the method shown in the step (1), and biomarker series concentration standard working solutions of at least three concentrations are prepared with matrix solutions of the blank samples within the range of 2-100 micrograms per liter; (3) liquid chromatogram-tandem mass spectrometry (LC-MS/MS) determination. A dispersive solid-phase extraction technique is used for the method, a sample pre-processing method capable of avoiding interference of matrixes in the samples easily, conveniently, quickly and effectively is developed, the pre-processing method is combined with the high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and is used for qualitative confirmation and quantitative detection of content of the biomarkers in animal tissue and urine, the recovery rate of the method ranges from 4.2% to 106.2%, the average relative standard deviation (RSD) ranges from 6.4% to 13.0%, the limit of detection is 20.0 microgram per kilogram, the limit of quantitation is 50.0 microgram per kilogram, and the method has the advantages of being easy and convenient to operate, quick, accurate, high in sensitivity and good in repeatability.

Description

technical field [0001] The present invention relates to a method for the determination of a group of biomarkers, more specifically a method for qualitatively and quantitatively measuring the content of biomarkers in biological tissues or urine products by using high performance liquid chromatography-tandem mass spectrometry (LC-MS / MS), The invention belongs to the technical field of biomarker determination. Background technique [0002] In recent years, with the continuous expansion of the scope of use of deltamethrin, the toxicological research of deltamethrin has also expanded from the simple selection of lower organisms as the experimental object to study the mechanism of neurotoxicity, and expanded to the three effects on mammals, Metabolic processes and other multi-level research. In order to assess its ecological risk, it is necessary to develop indicator parameters for biological sub-lethal effects to more accurately assess and predict its toxicity to environmental o...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 曹文卿静平李建军林黎明杨桂朋封立平
Owner INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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