A remote-controllable confocal microscope imaging device

Abstract

The invention relates to a remotely-controlled confocal microscopy imaging device. The remotely-controlled confocal microscopy imaging device comprises a laser transmitter, a dichroic mirror mechanism, a beam splitter mirror mechanism, an objective lens, a three-dimensional nanometer displacement platform, an optical filter, a slit mechanism and a photomultiplier, and further comprises a master controller and a plurality of imaging terminals, wherein the master controller is in communication connection with the laser transmitter, the dichroic mirror mechanism, the beam splitter mirror mechanism, the three-dimensional nanometer displacement platform, the slit mechanism and the photomultiplier, and the imaging terminals are in communication connection with the master controller. According to the remotely-controlled confocal microscopy imaging device, the master controller controls and records the position of each optical component, and each component in the confocal microscopy imaging device is precisely and rapidly controlled so that the accurate, stable and rapid imaging quality can be ensured; due to the fact that the master controller is in communication connection with the imaging terminals, the confocal microscopy imaging device is more flexible and variable to use, and has the functions of remote operation, remote assistance guidance and remote maintenance.

Description

technical field [0001] The invention relates to an imaging device, in particular to a laser scanning confocal microscope imaging device for imaging fluorescent substances, in particular to a remote controllable confocal microscope imaging device. Background technique [0002] As the basic unit of life activities, cells are the key to in-depth study of life processes. Understanding the conformational changes and precise positioning of various macromolecules in living cells and revealing the interaction relationship between biomolecules is of great significance for studying disease mechanisms and promoting the development of biomedical science and related industries. The key to realizing these lies in the observation and detection of cells. [0003] At present, Laser Scanning Confocal Microscopy (LSCM) is an important tool for in situ observation of cells. It is an effective technical means for studying submicron microstructures. It is a necessary large-scale scientific rese...

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Problems solved by technology

[0004] However, due to the high price of LSCM, not all laboratories can meet these experimental conditions. For scientific research units that do not have LSCM systems but have needs for LSCM imaging, they need to reserve and share their LSCM with other scientific research institutions that have LSCM systems. Laboratory, and assign designated personnel to the laboratory where the LSCM is located to perform imaging operations

As for the LSCM system itself, every time the imaging material is replaced, researchers need to spend a lot of time and energy to manually adjust each optical component, which not only reduces the experimental efficiency, but also affects the reproducibility of the experimental results. A lot of manpower and material resources were wasted

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