Induction method capable of promoting accumulation of salvianolic acid in hairy roots of Salvia miltiorrhiza

A technology of salvianolic acid and hairy root, which is applied in the induction field that can promote the accumulation of salvianolic acid in hairy roots of Salvia miltiorrhiza, and can solve the problems of promoting the accumulation of salvianolic acid and not informing sodium nitroprusside, etc.

Active Publication Date: 2017-05-03
ZHEJIANG SCI-TECH UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0010] However, the current prior art has not informed that sodium nitroprusside can promote the accumulation of salvianolic acid in the hairy roots of Salvia miltiorrhiza

Method used

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  • Induction method capable of promoting accumulation of salvianolic acid in hairy roots of Salvia miltiorrhiza
  • Induction method capable of promoting accumulation of salvianolic acid in hairy roots of Salvia miltiorrhiza
  • Induction method capable of promoting accumulation of salvianolic acid in hairy roots of Salvia miltiorrhiza

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Embodiment 1

[0031] Embodiment 1, a kind of induction method that can promote the salvianolic acid (mainly comprising rosmarinic acid and salvianolic acid B) accumulation in the hairy root of Salvia miltiorrhiza miltiorrhiza, carries out the following steps successively:

[0032] 1), initial cultivation of salvia miltiorrhiza hairy roots:

[0033] In an ultra-clean bench, weigh 0.2 g of Salvia miltiorrhiza root, put it into 50 ml of 6,7-V medium (100 ml Erlenmeyer flask, pH: 5.8), shaker at 110 rpm, and culture in the dark at 25°C for 18 sky.

[0034] 2), the continued cultivation of salvia miltiorrhiza root:

[0035] After the Salvia miltiorrhiza root was cultured for 18 days, sodium nitroprusside was added to make the final concentration of sodium nitroprusside in the 6,7-V medium 100 μM; culture was induced for 6 days at 110 rpm, 25° C., and in the dark.

[0036] After 6 days of induction, the hairy roots of Salvia miltiorrhiza were collected, rinsed with distilled water, blotted dry ...

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Abstract

The invention discloses an induction method capable of promoting the accumulation of salvianolic acid in the hairy root of Salvia miltiorrhiza, comprising the following steps: 1), initial culture of the hairy root of Salvia miltiorrhiza: inoculate 0.15 ~0.2g hairy root of Salvia miltiorrhiza, cultivated in the dark for 17~19 days under the culture condition of 100~150rpm, 24~26°C; The final concentration of sodium nitroprusside in the V medium is 90-110 μM; culture in dark for 5-7 days under the culture conditions of 100-150 rpm and 24-26°C. The method of the invention can promote the accumulation of salvianolic acid (mainly including rosmarinic acid and salvianolic acid B) in the hairy root of Salvia miltiorrhiza.

Description

technical field [0001] The invention relates to an induction method capable of promoting the accumulation of rosmarinic acid and salvianolic acid B in hairy roots of Salvia miltiorrhiza. Background technique [0002] Salvia miltiorrhiza (Salvia miltiorrhiza) is the root and rhizome of Salvia miltiorrhiza, which has the effects of promoting blood circulation and removing blood stasis, clearing heat and calming nerves, nourishing blood and nourishing qi. Salvianolic acid is one of the active ingredients of Danshen, mainly including rosmarinic acid and salvianolic acid B. Due to its outstanding performance and non-toxic side effects in the treatment of cardiovascular diseases, the application and research of Danshen are becoming more and more extensive. [0003] Currently, the hairy root of Salvia miltiorrhiza can be cultured in 6,7-V medium. Generally, 0.15-0.2 g of fresh hairy roots are weighed and inoculated into 50 mL of 6,7-V medium for dark culture. The hairy root of S...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 杨东风梁宗锁杜旭红刘岩王瑞杰何燕峰倪春红
Owner ZHEJIANG SCI-TECH UNIV
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