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A kind of preparation method of wheat germ cell active protein

A technology of wheat germ protein and cell activity, which is applied to the preparation method of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problems that hinder the use of wheat germ protein solution, and achieve high active protein content, simple method, and high activity. high effect

Active Publication Date: 2017-11-03
BEIJING 7SHOW TIMES TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the existing wheat germ protein solution contains some miscellaneous proteins, such as fibrin, some glycoproteins, etc., which hinder the use effect of the wheat germ protein solution.

Method used

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  • A kind of preparation method of wheat germ cell active protein
  • A kind of preparation method of wheat germ cell active protein
  • A kind of preparation method of wheat germ cell active protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Soak the wheat grains in distilled water, cultivate them at 28°C, cultivate them at an air humidity of 80%, cultivate them for 3 days, and crush them to 100 mesh to obtain a crushed mixture;

[0055] centrifuging the pulverized mixture at a speed of 5000r / min to obtain the first supernatant;

[0056] centrifuging the first supernatant at a speed of 8000r / min to obtain a second supernatant containing germ cells;

[0057] enzymatically hydrolyze the second supernatant, the temperature of enzymolysis is 27°C, and the time is 1h;

[0058] Wherein, the enzymolysis solution used in the enzymolysis contains carbohydrase, protease, lipase, 3-phytase;

[0059] The addition of carbohydrase is 1.2% of the second supernatant weight; The addition of protease is 0.1% of the second supernatant weight; The addition of lipase is 0.5% of the second supernatant weight; 3-muscle The amount of alcohol hexaphosphatase added is 0.15% of the weight of the second supernatant;

[0060] Under ...

Embodiment 2

[0071] Soak the wheat grains in distilled water, cultivate them at 35°C, cultivate them at an air humidity of 85%, cultivate them for 2 days, and crush them to 80 mesh to obtain a crushed mixture;

[0072] centrifuging the pulverized mixture at a speed of 5000r / min to obtain the first supernatant;

[0073] centrifuging the first supernatant at a speed of 8000r / min to obtain a second supernatant containing germ cells;

[0074] enzymolyze the second supernatant, the temperature of enzymolysis is 25°C, and the time is 2h;

[0075] Wherein, the enzymolysis solution used in the enzymolysis contains carbohydrase, protease, lipase, 3-phytase;

[0076] The addition of carbohydrase is 0.8% of the second supernatant weight; The addition of protease is 0.05% of the second supernatant weight; The addition of lipase is 0.4% of the second supernatant weight; 3-muscle The amount of alcohol hexaphosphatase added is 0.05% of the weight of the second supernatant;

[0077] Under the condition...

Embodiment 3

[0088] Soaking wheat grains in distilled water, cultivating at 30°C, cultivating at an air humidity of 85%, cultivating for 60 hours, crushing to 100 mesh, and obtaining a crushed mixture;

[0089] centrifuging the pulverized mixture at a speed of 5000r / min to obtain a first supernatant;

[0090] centrifuging the first supernatant at a speed of 8000r / min to obtain a second supernatant containing germ cells;

[0091] Enzymolyze the second supernatant, the temperature of the enzymolysis is 26°C, and the time is 1.5h;

[0092] Wherein, the enzymolysis solution used in the enzymolysis contains carbohydrase, protease, lipase, 3-phytase;

[0093] The addition of carbohydrase is 1% of the second supernatant weight; The addition of protease is 0.05% of the second supernatant weight; The addition of lipase is 0.5% of the second supernatant weight; 3-muscle The amount of alcohol hexaphosphatase added is 0.1% of the weight of the second supernatant;

[0094] Under the condition of 4°C...

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Abstract

The invention relates to the field of active protein preparation, in particular to a method for preparing active protein from wheat germ cells, comprising the following steps: using 35% ammonium sulfate solution to pre-precipitate the wheat germ protein solution, and then centrifuging to obtain a supernatant; Saturated ammonium sulfate solution fully precipitates the supernatant, centrifuges to obtain the precipitate and supernatant; dissolves the precipitate in PBS solution and dialyzes to remove ammonium sulfate to obtain the product. The preparation method first selects a specific concentration of ammonium sulfate solution to precipitate miscellaneous proteins in the wheat germ protein solution such as fibrin, a part of glycoprotein and a part of other proteins, and removes them by centrifugation; Glycoproteins, glycoprotein peptides and some growth factors are precipitated, and the obtained precipitate is the wheat germ cell active protein; finally, the ammonium sulfate in the precipitate is removed by dialysis, and the pure wheat germ cell active protein is obtained.

Description

technical field [0001] The invention relates to the field of active protein preparation, in particular to a method for preparing wheat germ cell active protein. Background technique [0002] Wheat germ contains flavonoids, such as kaempferol, myricetin, luteolin, catechins, bitter terpenes, 17 amino acids, and 25 elements such as potassium, manganese, phosphorus, calcium, and iron. It has remarkable convenient effects in anti-aging and anti-oxidation. [0003] In the past, the extraction of wheat germ protein mainly used the method of salt-dissolving alkali extraction and acid precipitation. However, this method requires a large amount of acid and alkali, and discharges a large amount of nutrients such as sugar and acid-base wastewater, which makes post-processing difficult and seriously pollutes the environment. Moreover, it is easy to denature the protein during the extraction process; the existing wheat germ protein extraction adopts the reverse micellar method, and the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/36C07K1/34C07K1/30C12P21/06
Inventor 黄海军
Owner BEIJING 7SHOW TIMES TECH