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A lactobacillus fermentum strain suo capable of regulating intestinal movement and preventing constipation and its application

A technology of fermenting Lactobacillus and intestinal motility, applied in the field of microbiology

Active Publication Date: 2017-12-26
江苏新申奥生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these patents or patent applications do not fully relate to microorganisms that have the properties of regulating intestinal motility, preventing constipation, etc.

Method used

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  • A lactobacillus fermentum strain suo capable of regulating intestinal movement and preventing constipation and its application
  • A lactobacillus fermentum strain suo capable of regulating intestinal movement and preventing constipation and its application
  • A lactobacillus fermentum strain suo capable of regulating intestinal movement and preventing constipation and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1: Isolation, purification and preliminary determination of Lactobacillus fermentum strain suo.

[0055] This embodiment is carried out according to the following steps:

[0056] (1) Separation and purification of Lactobacillus fermentum strain suo.

[0057] Draw 500 μL of traditional fermented yak yogurt sample by aseptic operation and add it to 5 mL sterilized saline to make a uniform dilution of 1:10, and continue to make a certain proportion of dilution. Select the appropriate gradient diluent, draw 100 μL each into the MRS solid plate medium with a sterile pipette tip, and incubate at 30°C for 48-72 hours, observe and record the colony morphology as follows: figure 1 shown.

[0058] Use an inoculation loop (or a sterilized toothpick) to pick different colonies from the surface and interior of the plate and inoculate them in the MRS liquid medium, and culture them on a shaker at 30°C and 300r / min for 24-48h; repeat the above steps for continuous activation...

Embodiment 2

[0064] Example 2: In vitro screening of Lactobacillus fermentum strain suo.

[0065] (1) Screening of probiotics resistant to pH 3.0 artificial gastric juice.

[0066] Preparation of artificial gastric juice: 0.2% NaCl, 0.35% pepsin, adjust the pH value to 3.0 with 1M HCl, filter and sterilize with a vacuum pump in an aseptic operating table for later use.

[0067] Determination of the tolerance of probiotics to artificial gastric juice: take 5mL of the activated strain culture solution, pour it into a sterilized 10mL centrifuge tube in a sterile operating table, collect the bacteria by centrifugation at 3000r / min for 10min, add 5mL of sterilized Mix physiological saline to make bacterial suspension, take 1mL bacterial suspension and 9mL artificial gastric juice with pH3. Samples were taken, poured into MRS agar medium and cultured at 37°C for 48h. The number of viable bacteria was determined by plate counting method, and the survival rate (%) was calculated: survival rate (...

Embodiment 3

[0081] Example 3: Activated carbon induced constipation experiment.

[0082] Feed healthy Kunming mice, 6 weeks old, weighing 23±2g, 50 females. After feeding the basal diet for 5 days, the mice were randomly divided into 5 groups according to body weight, namely: normal group, constipation control group, bisacodyl group, Lactobacillus bulgaricus group, and high and low intragastric dosage Lactobacillus fermentum strain suo group, 10 in each group. Raise them in stainless steel cages, keep room temperature at 24±2°C, relative humidity at 50±10%, and light-dark rotation for 12 hours (lighting at 8:00-20:00). During the whole experimental period, the normal group and the constipation control group were fed with the basal diet, and the mice in the experimental group were fed with 1.0×10 9 CFU / kg gavage probiotics. Two weeks later, at 9:00 every morning except the normal group, mice in the other five groups were given 10% activated carbon ice water at 2°C, 0.2 mL / mouse, once a ...

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Abstract

The invention discloses a Lactobacillus fermentum strain suo which can regulate intestinal movement and prevent constipation. The preservation number is CCTCC NO: M 2013511 and its application. After 3 hours in gastric juice, the survival rate reached 92.46±4.06%. It can grow slowly under 1.0% concentration of bile salt, and the growth efficiency reaches 17.36±1.19% of the culture without bile salt. The hydrophobicity of Lactobacillus fermentum strain suo cells also reached 68.44± 2.48%, normal growth in the human intestine. Lactobacillus fermentum strain suo can slow down the weight loss trend of mice caused by constipation, accelerate the time of melena, improve the small intestine propulsion rate, increase serum MTL, Gas, ET, AchE, SP and VIP factor levels to varying degrees, reduce SS factor, The trend of increasing food intake and drinking water, reducing the amount of defecation, the number of defecation particles and the water content of feces is similar to that of constipation drugs, showing a certain effect of preventing constipation.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Lactobacillus fermentum strain suo capable of regulating intestinal motility and preventing constipation and its application. Background technique [0002] Lactobacillus is closely related to human life and is one of the beneficial microorganisms widely used in food fermentation, industrial lactic acid fermentation and medical care. Many of the now recognized Lactobacillus and Bifidobacterium genera have specific favorable properties and genetic traits, showing extremely strong probiotic properties. Lactobacillus can not only improve the nutritional value of food, improve the flavor of food, prolong the storage time, but also improve the functional properties of food. It can improve the digestibility and biological value of food and promote digestion and absorption by decomposing protein, sugar and synthesizing vitamins in food. It can also reduce the content of choles...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23C9/123A23C9/16A23P10/30C12R1/225
CPCA23C9/123A23C9/13A23C9/16A23V2002/00C12R2001/225C12N1/205A23V2400/143A23V2200/30A23V2200/32
Inventor 索化夷李键骞宇赵欣陈炼红兰道亮谢婕
Owner 江苏新申奥生物科技有限公司
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