An identification method and application of deer antler powder in different sections
An identification method and deer antler powder technology are applied in the field of quality identification of deer antler products, which can solve the problems of not being widely used, unobtainable quality, and consumer trust, and achieve the effect of ensuring health care value and efficacy.
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Embodiment 1
[0040] Embodiment 1 Determination of ash content in different parts of velvet antler
[0041] 1) Divide the dried velvet antler into 4 sections according to the traditional method, take 1 sample at each end of each section, a total of 8 samples, marked as 1-8 from bottom to top, each sample has a mass of 5g, Crush and filter through a No. 2 sieve.
[0042] 2) Place the sample in a crucible that is burned to a constant weight (a), and weigh it as b, accurate to 0.01g. Burn in a muffle furnace at 550°C for about 4 hours, completely ash to constant weight, and weigh it as c.
[0043] 3) According to the formula x=(c-a) / (b-a)ⅹ100%, calculate the ash content of each part.
[0044] 4) Repeat operation step 1) to step 3) 3 times, obtain 3 parallel data, take the average, represent the ash content of each section, the result is as follows figure 1 shown. From figure 1 It can be seen that the ash content is not linear from top to bottom.
Embodiment 2
[0045] Embodiment 2 Establishment of ECV value database in different sections of velvet antler
[0046] 1) Extract the fat-soluble components and water-soluble components of different sections of velvet antler
[0047] The velvet antler is divided into 4 sections according to the traditional method, which are respectively wax slice area, powder slice area, blood slice area and bone slice area, and each slice area is ground into powder and filtered through a 45-mesh sieve. Take 0.5g from each section and use the Soxhlet extraction method to determine the content of fat-soluble substances (L). Dissolve 0.5 g of each section into 10 ml of phosphate buffer (0.05 M, pH 6.9), fully dissolve, centrifuge, and take the supernatant for gel chromatography analysis. A known concentration of cytochrome C was used as an internal reference.
[0048]2) Use the formula to calculate the index value (LI) of the fat-soluble substance and the index value (WI) of the water-soluble substance in ea...
Embodiment 3
[0060] Example 3 Verifying the known ECV values of different parts of velvet antler
[0061] 1) Take 8 samples of dried velvet antler at equal distances from top to bottom, and mark them as No. 1-8 from top to bottom, grind the samples into powder, and filter through a 45-mesh sieve. 0.5 g of each was extracted by Soxhlet extraction to determine the content of fat-soluble substances (L). Dissolve 0.5 g of each section into 10 ml of phosphate buffer (0.05 M, pH 6.9), fully dissolve, centrifuge, and take the supernatant for gel chromatography analysis. A known concentration of cytochrome C was used as an internal reference.
[0062] 2) Use the formula to calculate the index value (LI) of the fat-soluble substance and the index value (WI) of the water-soluble substance in each section. This method sets the maximum value of the active ingredient of velvet antler to 100 (the value of the active ingredient at the tip of the antler is 100, The active ingredient values in the re...
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