Control method of constant salt concentration in sand matrix for maize germination test

Abstract

The invention relates to a control method of constant salt concentration in a sand matrix for a maize germination test. The method comprises the following steps: (1) preparing the sand matrix; (2) preparing germination boxes; and (3) preparing a Hoagland nutrient solution with corresponding salt concentration and a NaCl water solution with the same concentration from NaCl, firstly, adding certain mL of Hoagland nutrient solution with corresponding salt concentration prepared from NaCl to each germination box at the current day, adding the same weight of distilled water at the third day, the seventh day, the eleventh day, the thirteenth day and the seventeenth day according to the weight loss of the germination boxes, adding the same weight of prepared NaCl water solution at the fifth day according to the weight loss of the germination boxes, and adding the same weight of Hoagland nutrient solution with the corresponding NaCl concentration at the ninth day and the fifteenth day according to the weight loss of the germination boxes. According to the control method, moisture and nutrients required for the maize germination test are ensured; and the maize germination test is performed at constant salt concentration.

Description

technical field [0001] The invention belongs to the technical field of salt tolerance analysis at the seedling stage of corn, in particular to a method for controlling constant salt concentration under a sand matrix in a corn germination test. Background technique [0002] When evaluating the salt tolerance index of corn seedlings, the germination test of corn seeds under salt stress is usually carried out in the laboratory. The germination test of large seeds is generally cultivated in a sand bed (fine sand of 0.05-0.8 mm). The method of stress conditions is to add a corresponding concentration of salt solution to the sand bed, but with the germination, emergence, growth and environmental factors of the seeds, the salt concentration of the sand bed will change, so that a certain constant salt solution cannot be accurately obtained in the test. stress concentration test data, so to explore the change of salt content in the sand bed with seed germination, so as to establish a...

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Problems solved by technology

[0002] When evaluating the salt tolerance index of corn seedlings, the germination test of corn seeds under salt stress is usually carried out in the laboratory. The germination test of large seeds is generally cultivated in a sand bed (fine sand of 0.05-0.8 mm). The method of stress conditions is to add a corresponding concentration of salt solution to the sand bed, but with the germination, emergence, growth and environmental factors of the seeds, the salt concentration of the sand bed will change, so that a certain constant salt solution cannot be accurately obtained in the test. stress concentration test data, so to explore the change of salt content in the sand bed with seed germination, so as to establish a system to maintain the stability of salt stress concentration conditions, for the identification and evaluation of corn seedling salt tolerance, corn seedling salt tolerance In-depth analysis of the mechanism is of great importance

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