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Double cross-linking agent amb-1 magnetosome drug carrier, preparation method and application thereof

A double cross-linking agent, AMB-1 technology, applied in the direction of pharmaceutical formulations, medical preparations containing active ingredients, organic active ingredients, etc., can solve the problems of limited encapsulation efficiency, application restrictions, low drug loading, etc., to achieve Long release period, convenient operation and good biocompatibility

Active Publication Date: 2017-07-21
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of the magnetosome AMB-1 as a drug carrier has been reported, but at present, a single chemical cross-linking agent is mostly used, which is highly toxic, and the drug loading is as low as about 10%, and the encapsulation efficiency is limited. Equipped with a single drug, its application is greatly limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Accurately weigh the purified and freeze-dried AMB-1 magnetosome particles, the AMB-1 magnetosomes are derived from Magnetospirillum magneticum AMB-1 magnetotactic bacteria, disperse them in PBS solution with pH 7.4, add AMB - 1 mg / mL poly-L-glutamic acid solution with the same mass as magnetosome particles, 50W ultrasonic dispersion for 5min to fully mix, add genipin to make the concentration in the mixed solution 0.5%; continue 50W ultrasonic dispersion The ultrasound was repeated 10 times for 1 minute with an interval of 5 minutes, and the mixed solution after ultrasound was placed in a shaker at 37° C. and 60 rpm for 72 hours to obtain the double crosslinking agent AMB-1 magnetosome drug carrier.

Embodiment 2

[0026] Accurately weigh the purified and freeze-dried AMB-1 magnetosome particles, the AMB-1 magnetosomes are derived from Magnetospirillum magneticum AMB-1 magnetotactic bacteria, disperse them in PBS solution with pH 7.4, add AMB - 1 mg / mL poly-L-glutamic acid solution with the same mass as magnetosome particles, 50W ultrasonic dispersion for 5min to fully mix, add genipin to make the concentration in the mixed solution 0.05%; continue 50W ultrasonic dispersion The ultrasound was repeated 10 times for 1 minute with an interval of 5 minutes, and the mixed solution after ultrasound was placed in a shaker at 37° C. and 60 rpm for 48 hours of cross-linking to obtain the double cross-linking agent AMB-1 magnetosome drug carrier.

Embodiment 3

[0028] Accurately weigh the purified and freeze-dried AMB-1 magnetosome particles, the AMB-1 magnetosomes are derived from Magnetospirillum magneticum AMB-1 magnetotactic bacteria, disperse them in PBS solution with pH 7.4, add AMB - 1 mg / mL poly-L-glutamic acid solution with the same mass as magnetosome particles, 50W ultrasonic dispersion for 5min to fully mix, add genipin to make the concentration in the mixed solution 0.1%; continue 50W ultrasonic dispersion Repeat the ultrasound 10 times for 1 minute with an interval of 5 minutes, and place the mixed solution after ultrasound in a shaker at 37° C. and 60 rpm for 12 hours to obtain the double crosslinking agent AMB-1 magnetosome drug carrier.

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Abstract

The invention discloses a double cross-linking agent AMB-1 magnetosome drug carrier, and a preparation method and application thereof. The double cross-linking agent AMB-1 magnetosome drug carrier employs an AMB-1 magnetosome possessing a natural lipid double-molecular-membrane layer as a carrier, employs genipin and poly(L-glutamic acid) as a double-molecule cross-linking material, so that the double cross-linking agent AMB-1 magnetosome drug carrier is formed, and is capable of carrying a single medicine or multiple medicines and helps to construct a targeting drug system. A natural double cross-linking agent is employed, the toxicity is low and the biocompatibility is good, the prepared double cross-linking agent AMB-1 magnetosome drug carrier is improved in drug loading and entrapment rate compared with a conventional technology, efficient loading of a single or multiple medicines is realized, burst release effect does not exist, the release period is long, the medicine self efficacy is not influenced, the effects of medicine combined usage and synergic interaction are realized while targetable drug delivery is realized, and also the preparation technology is simple, convenient to operate, green and environment-friendly, and possesses wide application prospect.

Description

technical field [0001] The invention relates to a double cross-linking agent AMB-1 magnetosome drug carrier, its preparation method and application. Background technique [0002] The research on drug carriers has always been one of the hot spots in the field of medicine, in which the development and use of new materials plays an important role in the research of drug carriers. Magnetosomes are single magnetic domain crystals coated with phospholipid bilayer membranes, with diameters ranging from tens to hundreds of nanometers, and the main components include Fe 3 o 4 , FeS, Fe 3 S 4 or FeS 2 . Magnetosomes synthesized by different magnetotactic bacteria are different in size, quantity, composition, and morphological structure, which means they are species-specific. A magnetosome membrane derived from Magnetospirillum magneticum AMB-1 magnetotactic bacteria contains a large number of proteins and amino acids, these proteins and amino acids (such as primary amino groups,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/59A61K31/704A61K31/7068
Inventor 刘源岗王士斌邓琼嘉代晴蕾谢茂彬陈爱政吴文果
Owner HUAQIAO UNIVERSITY
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