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Method for extracting andrographolide from common andrographis herb and andrographolide finished products

A technology of andrographolide and andrographolide, which is applied in the field of extraction of andrographolide, can solve the problems of expensive equipment, increased production cost, large amount of solvent usage, etc., and achieve the effect of reducing production cost, reducing equipment cost, and easy separation and purification

Inactive Publication Date: 2015-04-22
李宏 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many reports on the extraction methods of andrographolide, including water extraction, alcohol extraction, alkaline water extraction, etc. These processes are simple to operate, but have low extraction efficiency, low raw material utilization rate, large solvent usage and Impurities are difficult to remove, and other extraction methods, such as supercritical extraction, ultrasonic-assisted extraction, microwave-assisted extraction, resin adsorption, etc., although high-purity andrographolide can be obtained, the required equipment is expensive and greatly Increased production costs, and difficult to apply to industrial mass production

Method used

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  • Method for extracting andrographolide from common andrographis herb and andrographolide finished products
  • Method for extracting andrographolide from common andrographis herb and andrographolide finished products
  • Method for extracting andrographolide from common andrographis herb and andrographolide finished products

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Prepare the enzyme solution of acid cellulase and acid xylanase compound enzyme, take acid cellulase 300u / g (andrographis paniculata dry basis), acid xylanase 600u / g (andrographis paniculata dry basis), add water and stir to dissolve , configured as a compound enzyme solution with a concentration of 3%. Take 10kg of Andrographis paniculata and slice it into 1-2cm, spray the compound enzyme solution evenly on the Andrographis paniculata slices, mix evenly, keep warm at 35°C for 0.4h, 0.5h, 1h, 2h, 2.5h, after the reaction, Add concentration and be 85% ethanol and reflux extract twice, each 1.5 hours, the ethanol consumption for the first time is 8 times of the dry weight of Andrographis paniculata, and the second ethanol consumption is 6 times of the dry weight of Andrographis paniculata, merge the reflux liquid twice, The reflux liquid was filtered and collected to obtain an extract, which was set aside. The remaining Andrographis paniculata residue (abbreviated as res...

Embodiment 2

[0049] Prepare the enzyme liquid of acid cellulase and acid xylanase compound enzyme, take acid cellulase 1200u / g (andrographis paniculata) and acid xylanase 900u / g (andrographis paniculata), add water and stir to dissolve , configured as compound enzyme solutions with concentrations of 1%, 3%, 5%, 10%, and 15%, respectively. Get 10kg of Andrographis paniculata coarse powder, spray and mix evenly with compound enzyme solution, heat preservation and enzymolysis treatment at 45°C for 2h, after the reaction is over, add 85% ethanol to reflux extraction twice, each time for 1.5 hours, the first ethanol consumption is 8 times of the dry weight of Andrographis paniculata, and the second ethanol dosage is 6 times of the dry weight of Andrographis paniculata, the two reflux liquids are combined, and the reflux liquid is filtered to obtain the extract, which is set aside. The remaining Andrographis paniculata residue (residue for short) is washed with clear water, dried, and utilizes h...

Embodiment 3

[0056] Prepare acid cellulase and acid xylanase compound enzyme, get acid cellulase 900u / g (andrographis paniculata dry basis), acid xylanase 2400u / g (andrographis paniculata dry basis), add water and stir to dissolve, configure Concentration of 5% compound enzyme solution. Take 10kg of Andrographis paniculata powder, spray and mix it evenly with compound enzyme solution, heat it at 30°C, 35°C, 45°C, 50°C, 55°C for 2 hours, and add 90% ethanol to reflux to extract twice , 1.5 hours each time, the first ethanol consumption is 8 times of the dry weight of Andrographis paniculata, the second ethanol consumption is 6 times of the dry weight of Andrographis paniculata, the reflux liquid of the two times is combined, and the reflux liquid is filtered to obtain the extract ,stand-by. The remaining Andrographis paniculata residue (abbreviated as residue) was washed with clear water, dried, and the content of andrographolide in the residue was determined by high performance liquid chr...

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Abstract

The invention relates to the technical field of andrographolide extraction, and in particular relates to a method for extracting andrographolide from common andrographis herb and andrographolide finished products obtained by using the method. The method comprises the following steps: performing enzymatic hydrolysis pretreatment on the whole herb of common andrographis herb or common andrographis herb coarse powder by using a compound enzyme of acidic cellulose and acidic xylanase, wherein the enzymatic hydrolysis pretreatment comprises spraying and heat preservation treatment processes; and performing alcohol extraction treatment on the whole herb of common andrographis herb or the common andrographis herb coarse powder subjected to pretreatment. According to an extraction method disclosed by the invention, expensive equipment does not need to be used, and the extraction cost of andrographolide can be reduced.

Description

technical field [0001] The invention relates to the technical field of andrographolide extraction, in particular to a method for extracting andrographolide from andrographolide and a finished product of andrographolide obtained by the method. Background technique [0002] Andrographolide is the main active ingredient of Andrographis paniculata, which has antibacterial, anti-inflammatory, cough and asthma, heat-clearing and detoxifying, cooling blood and detumescence effects, especially for bacterial and viral upper respiratory tract infections and dysentery. Known as a natural antibiotic drug. Due to the complex structure of andrographolide and the difficulty of artificial synthesis, it is extracted from plant medicinal materials in China. [0003] At present, there are many reports on the extraction methods of andrographolide, including water extraction, alcohol extraction, alkaline water extraction, etc. These processes are simple to operate, but have low extraction effic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D307/60
CPCC07D307/60
Inventor 陈雪娇李宏岳昌林贾英华
Owner 李宏
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