Specific functional marker of rice sterility gene pms3 and its application

Abstract

The invention belongs to the field of molecular biology, and provides a specificity functional marker for a rice sterility gene pms3 and application of the specificity functional marker. The primer pms3-C of the wild type C and the primary pms3-G of the mutant type G can be specifically amplified according to the single base mutation of the pms3 function changes causing rice sterility and the sequence design of the G-C difference positions, a mismatch is introduced to the end position 3' so that the specificity can be enhanced, forward primers pms3F are designed on the upstream part and the downstream part of the end position 3' respectively, a backward primer pms3R is designed on the downstream part, the verification shows that the primer sequence of the specificity functional marker for the rice sterility gene pms3 is obtained, the allele relation of the pms3 in a rice template can be rapidly detected through electrophoresis when the PCR amplification is conducted through the marker, the specificity functional marker can be applied to purification of some rice thermo-sensitive genic male sterility, the purity identification of two-line hybrid rice, and the seed selection of the thermo-sensitive genic male sterility, and the breeding process of the two-line method is accelerated.

Description

technical field [0001] The invention belongs to the field of molecular biology and relates to a specific functional marker of rice sterility gene pms3 and its application. Background technique [0002] Rice is the main food crop in my country, and more than half of the population takes rice as the main ration. Hybrid rice is the main way to increase rice production and plays a pivotal role in my country's food security. Among them, compared with three-line hybrid rice, two-line hybrid rice has simplified seed production procedures, and fully utilizes indica-japonica heterosis, so it has higher yield potential. However, the fertility of the two-line male sterile line is affected by temperature and light, and when low temperature is encountered at the booting stage of seed production, it will self-fertilize and become firm, resulting in the purity of hybrid seeds exceeding the standard. Therefore, it is very important to purify and identify the purity of the two-line male st...

AI Technical Summary

Problems solved by technology

[0003] The research team of Zhang Qifa from Huazhong Agricultural University pointed out that it is a long non-coding RNA that controls the sterility of Nongken 58S, and the transcript 1 of LOC_12g36030 is pms3. Studies have shown that it is 1236bp in length and is related to specific male sterility under long-term light. RNA molecule LDMAR (long-day–specific male-fertility–associated RNA, non-coding RNA as the research object, this gene regulates light-sensitive male sterility in rice, referred to as LDMAR), under long-day conditions, sufficient LDMAR The amount of transcription is necessary to maintain normal pollen development, but due to a single base mutation that changes the secondary structure of LDMAR, the amount of transcription of LDMAR is reduced under specific long-day sunlight, resulting in premature programmed death of developing anthers, namely Produce PSMS (photoperiod-sensitive male sterility, thermosensitive male sterility)

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