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Rapid detection kit and method of urea

A technology for detecting kits and reagents, which is applied in the direction of material analysis through observation of the influence of chemical indicators, analysis through chemical reactions of materials, and analysis using chemical indicators to achieve good stability, high accuracy, and highly specific effect

Active Publication Date: 2015-04-22
GUANGDONG HUANKAI MICROBIAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, no effective elimination of Cu in this method has been seen. 2+ and residual chlorine interference reports

Method used

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  • Rapid detection kit and method of urea
  • Rapid detection kit and method of urea
  • Rapid detection kit and method of urea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 kit components

[0039] (1) Reagent Ⅰ: Weigh 4.0g disodium edetate and 2.0g trehalose, dissolve them in phosphate buffer solution with pH=7.5, dilute them into 100mL volumetric flasks, shake well and put them in dropper bottles .

[0040] (2) Reagent II: Weigh urease and dissolve it in deionized water, then add glycerol to prepare a 100U / mL urease solution, shake well and dispense into dropper bottles.

[0041] (3) Reagent III: Weigh 34.0g of anhydrous citric acid, 4.0g of sodium salicylate, and 2.0g of sodium nitroprusside, mix them thoroughly and pack them into 0.10g / bag.

[0042] (4) Reagent IV: Weigh 20.0g of lithium hydroxide, 4.0g of sodium dichloroisocyanurate, and 16.0g of anhydrous sodium sulfate, mix them thoroughly, and pack them into 0.10g / bag.

Embodiment 2

[0043] The preparation of embodiment 2 standard color card

[0044] 1) Prepare urea with concentrations of 0.5mg / L, 1.0mg / L, 1.5mg / L, 2.0mg / L, 2.5mg / L, 3.5mg / L, 5.0mg / L, 8.0mg / L with pure water standard solution;

[0045] 2) Take 10mL of the standard solution of each concentration into the colorimetric bottle, add 2 drops of reagent I and shake well;

[0046] 3) Add 2 drops of Reagent II dropwise, shake well, and let stand for 5 minutes;

[0047] 4) Add 1 packet of reagent III, shake well, and let stand for 2 minutes;

[0048] 5) Add 1 packet of reagent IV, shake well, and let stand for 15 minutes;

[0049] 6) After the reaction is completed, obtain the standard colors that appear when the urea standard solutions of various concentrations develop color, and use computer color matching and printing according to the color values ​​listed in the obtained Pantone standard colors to obtain standard color comparison cards, such as figure 1 shown.

Embodiment 3

[0050] Example 3 Kit operation method

[0051] Utilize the method for detecting the concentration of urea in swimming pool water with the test kit of the present invention, the steps are as follows:

[0052] 1) Take 10mL of the water sample to be tested into the colorimetric bottle, add 2 drops of reagent I dropwise, and shake well;

[0053] 2) Add 2 drops of Reagent II dropwise, shake gently, and let stand for 5 minutes;

[0054] 3) Add 1 packet of reagent III, shake gently, and let stand for 2 minutes;

[0055] 4) Add 1 packet of reagent IV, shake gently, and let stand for 15 minutes;

[0056] 5) Place the colorimetric bottle in the blank space 1cm above the colorimetric card, and compare it with the color of the colorimetric card to obtain the concentration of urea in mg / L of the measured water sample.

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Abstract

The invention discloses a rapid detection kit of urea. The rapid detection kit comprises a reagent I, a reagent II, a reagent III and a reagent IV, wherein the reagent I comprises EDTA disodium and trehalose; the reagent II is urease; the reagent III comprises anhydrous citric acid, sodium salicylate and sodium nitroprusside; the reagent IV comprises lithium hydroxide, dichlord isocyanurice acid and anhydrous sodium sulfate. According to the detection kit and a detection method, the interference of copper ions and residual chlorine in water of a swimming pool to a detection result can be effectively shielded, the measuring range is 0.5mg / L-8.0mg / L, the detection time is about 20 minutes, and the guarantee periods of the reagents at the room temperature are long; the kit is high in accuracy, strong in specificity, good in stability, convenient, and rapid and is applicable to the spot rapid detection of the swimming pool, and the detection result is close to that by adopting a national standard method, so that the kit has a good application prospect.

Description

technical field [0001] The invention relates to a urea rapid detection kit and a detection method, in particular to a kit and a detection method for rapid detection of urea concentration in swimming pool water. Background technique [0002] Relevant studies have shown that the main source of urea in swimming pools is urine excreted by humans, and nearly 87% of it is urea. In addition, sweat will also contain a small amount of urea. It can be seen that due to the special source of urea in swimming pool water, the load of swimming pools increases greatly in summer when swimmers are concentrated, and urea in swimming pool water exceeds the standard frequently; An objective indicator of pollution levels. As for the harm of urea, in fact, as a normal metabolite of the human body, the extremely low concentration of urea in swimming pool water will not cause direct pathogenic harm to the human body. In addition, the direct reaction between urea and disinfectant residual chlorine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N31/22G01N21/78
Inventor 黄报亮吴清平邓金花李方方
Owner GUANGDONG HUANKAI MICROBIAL SCI & TECH
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