Method for prevention and control of vegetable root-knot nematodiasis with mixed Sr18 biological nematocide

A root-knot nematode and nematocide technology, applied in the field of vegetable root-knot nematode prevention and control by mixed Sr18 bionematicide, which can solve the problem of unreasonable application time, aggravate plant infestation hazards, and increase soil salt pollution To achieve the effect of improving spraying time, increasing soil salt pollution, and reducing drug efficacy

Inactive Publication Date: 2015-04-29
TIANJIN NORMAL UNIVERSITY
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Problems solved by technology

[0006] Articles published on plos one by Huang Wenkun of the research group in 2014: Wen-Kun Huang, Jian-Hua Sun, Jiang-Kuan Cui, Gang-Feng Wang, Ling-An Kong, Huan Peng, Shu-Long Chen, De-Liang Peng . Efficacy Evaluation of Fungus Syncephalastrum racemosum and ematicide Avermectin against the Root-Knot Nematode Meloidogyne incognita on Cucumber [J]. Plos One, 2014,9(2):1-6.) The medicament fermentation scale in the article is small, and the formula is PS, Without adding malt extract, most of the substances formed under PS medium conditions are acidic substances, which are not conducive to the production of insecticidal substances that work under neutral conditions, and the pH of the fermentation filtrate is adjusted to 7.0 with 1.0mol / L NaOH, which will reduce Drug effect, increase soil salt pollution
At the same time, the control effect of Sr18 on nematodes is not fully reflected, which is related to the inappropriate proportion of pesticides used. Since the test site is a greenhouse with severe root-knot nematodes and the density of nematodes in the soil is high, if the dose of Sr18 is insufficient, the nematodes cannot be controlled within the effective range. In the soil, it will cause nematodes to reproduce again and increase the damage to the plants; moreover, the application time is not reasonable, and the application of the pesticide 2 days before transplanting does not reach the best time for the action of the pesticide, so it cannot be used The effect of the drug is fully exerted

Method used

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  • Method for prevention and control of vegetable root-knot nematodiasis with mixed Sr18 biological nematocide
  • Method for prevention and control of vegetable root-knot nematodiasis with mixed Sr18 biological nematocide
  • Method for prevention and control of vegetable root-knot nematodiasis with mixed Sr18 biological nematocide

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Experimental program
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Effect test

Embodiment 1

[0077] Preparation of Sr18 bionematicide:

[0078] (1) Activation culture of Sr18 bacteria and preparation of spore suspension: put the strains preserved in the ampoule tube into the slant medium, and culture at 26°C for 6 days; take the activated Sr18 slant, add 5ml of sterile water to wash the lower slant Pour the spores on the glass beads into the Erlenmeyer flask, shake for 1 minute, and count;

[0079] (2) 200L tank fermentation enlargement: use 50L fermentation tank as seed tank to prepare first-grade seeds: according to 2.75×10 6 / L spore concentration Put the spore suspension prepared in step (1) into a seed tank filled with 22L medium, and incubate at 26°C for 24h. Put the first-grade seeds into a 200L fermenter with 150L fermentation medium at a 4% inoculum volume, stirring at a speed of 198r / min, tank pressure at 0.05MPa, ventilation to maintain a dissolved oxygen level of 20%, and a fermentation time of 40 hours ; The fermentation medium (w / w) is: 30% potato dipp...

Embodiment 2

[0083] Adopt the method for mixing Sr18 bionematicide to prevent and control cucumber root-knot nematode disease, it is characterized in that carrying out according to following steps:

[0084] (1) The configuration of mixing Sr18 bionemicide: Sr18 8-fold concentrated water solution and abamectin emulsion are prepared at a ratio of 7.5:2 by weight;

[0085] (2) Application method: Rotate the soil before use, divide the plots according to the area of ​​the test plot, and then form a ridge. Take the corresponding Sr18 bionemicide and abamectin EC according to the configuration in step (1), and mix them well. , add water to dilute 10 times, spray the soil with a sprayer, and transplant after 3 days. For short-season vegetables, soil treatment is mainly carried out 3 days before transplanting;

[0086] (3) In the middle stage of planting, add 2 additional doses of medicine, the method is to irrigate with water, and the dose and ratio of the medicine remain unchanged;

[0087] (4)...

Embodiment 3

[0089] Adopt the method for mixing Sr18 bionematicide to prevent and control tomato root-knot nematode disease, it is characterized in that following steps are carried out:

[0090] (1) The configuration of mixing Sr18 bionemicide: the 8-fold concentrated water of Sr18 and the abamectin emulsion are prepared at a ratio of 7.5:2 by weight; that is, the dosage of Sr18 and avermectin emulsion is Sr18 7.5ml / ㎡ 2 +1.8% (w / w) Abamectin EC 2ml / ㎡ 2

[0091] (2) Application method: Rotate the soil before use, divide the plots according to the area of ​​the test plot, and then form a ridge. Take the corresponding Sr18 agent and avermectin EC according to the configuration in step (1), mix well, and add water After diluting 20 times, spray the soil with medical equipment, and transplant after 5 days of spraying. For short-season vegetables, soil treatment is mainly carried out 5 days before transplanting;

[0092] (3) In the middle stage of planting, add 1 application of medicine, the ...

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Abstract

The invention discloses a method for prevention and control of vegetable root-knot nematodiasis with mixed Sr18 biological nematocide. The method includes: preparing the mixed Sr18 biological nematocide from 8-time concentrated aqueous solution of Sr18 and avermectin emulsion according to a weight part ratio of 7.5:2; subjecting soil to rotary tillage before use of the mixed Sr18 biological nematocide, dividing plots according to areas of test plots, ridging, taking corresponding Sr18 agent and avermectin emulsion according to the preparation ratio at the step (1), uniformly mixing, adding clean water for diluting by 10-20 times, adopting spraying equipment for soil spraying, transplanting in 3-5 days after application of the mixed Sr18 biological nematocide, and additionally applying the nematocide for 1-2 times to long-season vegetables in the middle period of planting by irrigating the nematocide along with water and keeping the dosage and proportion unchanged; detecting conditions of vegetable root-knot nematodiasis. Experiment results show that root-knot nematodiasis can be effectively controlled by the method, consumption and utilization frequency of avermectins are greatly reduced, the picking period of cucumbers is greatly prolonged by about 3 months, and fruit setting rate of tomatoes is increased by 30%.

Description

[0001] This invention was funded by the National Natural Science Foundation of China (Project No. 31272019). technical field [0002] The invention relates to a mixing method of biopesticides, in particular to a method of mixing Sr18 bionematicides to prevent and control vegetable root-knot nematode diseases. The advantages of a long period of time, through the synergistic effect between the two, make up for the shortcomings of a single preparation, overcome the resistance of nematodes, improve the control effect of pesticides, prolong the harvesting period of crops, increase production and income significantly, and expand the use of pesticides scope. Background technique [0003] Plant nematodes are a kind of important plant pathogens distributed worldwide. They have a wide range of parasites and strong environmental adaptability. Almost every crop is attacked by plant nematodes. Among them, root-knot nematodes and cyst nematodes are the most harmful to crops. . Root-knot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G13/00
CPCA01G13/00
Inventor 孙建华冯欣孟庆恒任毅刘屾黄弢彭德良黄文坤郝永娟
Owner TIANJIN NORMAL UNIVERSITY
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