Method for prevention and control of vegetable root-knot nematodiasis with mixed Sr18 biological nematocide
A root-knot nematode and nematocide technology, applied in the field of vegetable root-knot nematode prevention and control by mixed Sr18 bionematicide, which can solve the problem of unreasonable application time, aggravate plant infestation hazards, and increase soil salt pollution To achieve the effect of improving spraying time, increasing soil salt pollution, and reducing drug efficacy
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Embodiment 1
[0077] Preparation of Sr18 bionematicide:
[0078] (1) Activation culture of Sr18 bacteria and preparation of spore suspension: put the strains preserved in the ampoule tube into the slant medium, and culture at 26°C for 6 days; take the activated Sr18 slant, add 5ml of sterile water to wash the lower slant Pour the spores on the glass beads into the Erlenmeyer flask, shake for 1 minute, and count;
[0079] (2) 200L tank fermentation enlargement: use 50L fermentation tank as seed tank to prepare first-grade seeds: according to 2.75×10 6 / L spore concentration Put the spore suspension prepared in step (1) into a seed tank filled with 22L medium, and incubate at 26°C for 24h. Put the first-grade seeds into a 200L fermenter with 150L fermentation medium at a 4% inoculum volume, stirring at a speed of 198r / min, tank pressure at 0.05MPa, ventilation to maintain a dissolved oxygen level of 20%, and a fermentation time of 40 hours ; The fermentation medium (w / w) is: 30% potato dipp...
Embodiment 2
[0083] Adopt the method for mixing Sr18 bionematicide to prevent and control cucumber root-knot nematode disease, it is characterized in that carrying out according to following steps:
[0084] (1) The configuration of mixing Sr18 bionemicide: Sr18 8-fold concentrated water solution and abamectin emulsion are prepared at a ratio of 7.5:2 by weight;
[0085] (2) Application method: Rotate the soil before use, divide the plots according to the area of the test plot, and then form a ridge. Take the corresponding Sr18 bionemicide and abamectin EC according to the configuration in step (1), and mix them well. , add water to dilute 10 times, spray the soil with a sprayer, and transplant after 3 days. For short-season vegetables, soil treatment is mainly carried out 3 days before transplanting;
[0086] (3) In the middle stage of planting, add 2 additional doses of medicine, the method is to irrigate with water, and the dose and ratio of the medicine remain unchanged;
[0087] (4)...
Embodiment 3
[0089] Adopt the method for mixing Sr18 bionematicide to prevent and control tomato root-knot nematode disease, it is characterized in that following steps are carried out:
[0090] (1) The configuration of mixing Sr18 bionemicide: the 8-fold concentrated water of Sr18 and the abamectin emulsion are prepared at a ratio of 7.5:2 by weight; that is, the dosage of Sr18 and avermectin emulsion is Sr18 7.5ml / ㎡ 2 +1.8% (w / w) Abamectin EC 2ml / ㎡ 2
[0091] (2) Application method: Rotate the soil before use, divide the plots according to the area of the test plot, and then form a ridge. Take the corresponding Sr18 agent and avermectin EC according to the configuration in step (1), mix well, and add water After diluting 20 times, spray the soil with medical equipment, and transplant after 5 days of spraying. For short-season vegetables, soil treatment is mainly carried out 5 days before transplanting;
[0092] (3) In the middle stage of planting, add 1 application of medicine, the ...
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