Method for isolating living cells of adipose tissue, pharmaceutical composition and use thereof, and cell bank

Abstract

The invention discloses an adipose tissue living cell separation method and a medical composition, as well as an application and cell bank of the medical composition, and aims to overcome the defect in the prior art that since an adipose tissue is chopped through a handheld cutter before living cells are separated from the adipose tissue, only a few number of the living cells can be separated, and the cell survival rate is low. The method comprises the following steps: the adipose tissue is provided; the adipose tissue is homogeneously chopped through a cutting device; a reagent is added into the chopped adipose tissue for hydrolysis; centrifugal separation is further conducted; a supernate is removed, so as to obtain a cell sediment. Therefore, the method can shorten the adipose tissue chopping time, avoid pollution caused by repeated use of the cutter, increase the number of the living cells obtained from per unit weight of the adipose tissue, and prevent the cell survival rate from decreasing. The method is applicable for separating the living cells from the adipose tissue.

Description

technical field [0001] The present invention relates to a method for separating living cells of adipose tissue, a pharmaceutical composition and its use, and a cell bank, especially to a method for rapidly and homogeneously chopping adipose tissue with a cutting device, so as to further separate living cells from adipose tissue. cells to increase the number of viable cells obtained per unit weight of adipose tissue without reducing cell viability. Background technique [0002] By the way, the source of mesenchymal stem cells can be isolated from many different tissues of the human body. For example, adipose tissue obtained by direct surgery or liposuction is a rich source of stem cells. Adiposetissue-derived stem cells (Adiposetissue-derived stem cells, ADSCs), which have the following advantages: the acquisition method is less invasive, less harmful to the human body, and the amount obtained at one time is large, and can be proliferated and cultured in vitro; in addition, a...

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Problems solved by technology

However, the method disclosed in this case also used scissors or tweezers to dismantle the orbital fat tissue, and it took a lot of time to cut up the fat tissue as much as possible to obtain more cell sediments; and relatively, The longer it operates the mincing step, the greater the effect on the viability of the cells in the aforementioned cell pellet

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