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A new type of environmentally friendly and non-toxic liquid-based cell preservation solution

A technology of liquid-based cell and preservation solution, which is applied in the field of new environment-friendly liquid-based cell preservation solution, which can solve the problems of medical staff safety hazards, environmental pollution, toxicity and harm, etc., and achieve cell shape intact, nuclei and cytoplasm clear, and cell preservation rate high effect

Inactive Publication Date: 2017-06-16
李东
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the existing cell preservation solution contains methanol, isopropanol and other harmful liquids, which are poisonous and harmful, and pollute the environment. Among them, 4 milliliters of methanol can cause blindness, and 10 milliliters can cause death, which brings certain dangers to medical workers. Security risks

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A new type of environment-friendly liquid-based cell preservation solution, composed of the following raw materials in parts by mass: 30% ethanol, 0.02% potassium chloride, 0.024% potassium dihydrogen phosphate, 0.144% disodium hydrogen phosphate, 0.8% sodium chloride, acetic acid Sodium 0.8%, acetic acid 0.8%, ethylenediaminetetraacetic acid 0.8%, bromhexine hydrochloride 0.03%, polyoxyethylene sorbitan monolaurate 0.8%, phenol 0.4%, and the remaining components are ultrapure water.

Embodiment 2

[0017] A new type of environment-friendly liquid-based cell preservation solution, composed of the following raw materials in parts by mass: 30% ethanol, 0.02% potassium chloride, 0.024% potassium dihydrogen phosphate, 0.144% disodium hydrogen phosphate, 0.8% sodium chloride, acetic acid Sodium 0.8%, acetic acid 0.8%, ethylenediaminetetraacetic acid 0.8%, bromhexine hydrochloride 0.02%, polyoxyethylene sorbitan monolaurate 0.8%, phenol 0.3%, and the remaining components are ultrapure water.

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PUM

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Abstract

The invention relates to a liquid-based cell preservation solution, which belongs to the technical field of medicaments, in particular to a novel environment-friendly liquid-based cell preservation solution. Including ultrapure water, pH buffering agent, osmotic pressure maintaining agent, antiseptic preservative, fixative for maintaining cell shape, metal chelating agent, and surfactant. The present invention has the advantages that: the main component is about 30% ethanol, does not contain methanol and isopropanol toxic and harmful components, is non-toxic, harmless and environmentally friendly; the cell preservation rate is high, and it can be stored for a long time at room temperature; the cell shape is intact, and the cytoplasm The boundary with the nucleus is clear and well-defined, the transparency of the cytoplasm and the nucleus is very good, the nucleus is very clear, and the integrity of the nucleic acid in the cell can be better preserved.

Description

technical field [0001] The invention relates to a liquid-based cell preservation solution, which belongs to the technical field of medicaments, in particular to a novel environment-friendly liquid-based cell preservation solution. Background technique [0002] At present, cervical cancer is still an important killer of women's health. Its incidence rate ranks second among female malignant tumors, second only to breast cancer. According to statistics in 2000, there are about 500,000 new cases of cervical cancer every year in the world, and there are more than 130,000 new cases in China, accounting for about a quarter of the world's new cases. About 20,000 to 30,000 people die from cervical cancer every year, and it is on the rise and younger. [0003] At present, Pap smears are commonly used for cervical cancer detection, but due to blood, mucus, inflammation and other factors in conventional Pap smears, the samples are often blurred and there are detection errors. Liquid-...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 李东唐永春于惠傅琳
Owner 李东