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Method for identifying Japanese ardisia herb in Chinese patent medicine for treating heat asthma

A technology of Chinese patent medicine and dwarf tea, which is applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of unsatisfactory expansion effect of silica gel G thin-layer plate, samples containing many impurities, and unclear spots, etc., to improve the expansion system. and inspection conditions, the effect of many impurities, and the effect of easily eliminating the interference of impurities

Active Publication Date: 2015-05-06
SHAN DONG DONG E E JIAO
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AI Technical Summary

Problems solved by technology

[0003] The original national medicine standard of this Chinese patent medicine does not have the identification item of Aodicha. When the pharmacopoeia quality standard for identification of Aidicha is applied to the Chinese patent medicine, there are the following problems: 1. Since the sample contains many impurities, and the petracenin component is in the water layer, After using methanol to extract, there are still many impurities
2. Since petracenin is an isocoumarin, the development effect of using silica gel G thin-layer plate is not ideal. 3. Since petracenin has no specific color developer, the spots are not clear and the reproducibility is poor after the color development method of the pharmacopoeia

Method used

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  • Method for identifying Japanese ardisia herb in Chinese patent medicine for treating heat asthma
  • Method for identifying Japanese ardisia herb in Chinese patent medicine for treating heat asthma

Examples

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Embodiment 1

[0029] The thin-layer chromatographic identification of embodiment 1 short ground tea medical material

[0030] Take 0.4g each of the medicinal herbs of Hebei, Hubei, Hunan, Sichuan, and Guangxi from 5 different producing areas, grind them finely, add 20ml of ethanol, ultrasonically extract for 30 minutes, filter, evaporate the filtrate to dryness, add 5ml of water to the residue to dissolve, and pass through the large Hole adsorption resin column, elute with 50ml of 35% ethanol, collect the eluent, evaporate to dryness, add methanol 1ml to dissolve the residue, and use the supernatant as the test solution. Take another petracenin reference substance, add methanol to make a solution containing 1mg per 1ml, and use it as the reference substance solution. According to the thin-layer chromatography (Appendix VIB of the Chinese Pharmacopoeia 2010 edition), draw 3 μl of each of the above two solutions, respectively. Spot on the same polyamide film, develop with ethyl acetate-acetic...

Embodiment 2

[0032] Example 2 TLC identification of the Chinese patent medicine for the treatment of febrile asthma

[0033] The preparation method of the Chinese patent medicine for treating fever asthma is: 265g of Ligustrum lucidum, 140g of Schisandra chinensis and 65% ethanol are heated and refluxed to extract twice, each time for 1.5 hours, the extracts are combined, and the recovered ethanol is concentrated to a relative density of 1.07-1.09 (60° C. ); take another 160g of ephedra, 210g of bitter almond, 170g of peach kernel for steam distillation, and collect the distillate for subsequent use. Dregs, 360g of Aodicha, 210g of Scutellaria baicalensis, 165g of Dilonga, 240g of Epimedium, 105g of licorice and decoction of five medicinal materials with water twice, 1 hour each time, combined the decoction and the above-mentioned distilled medicinal liquid, filtered, and the filtrate Concentrate to a relative density of 1.02-1.06 (60°C), combine the above three extracts, spray dry, add an...

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Abstract

The invention relates to a method for identifying Japanese ardisia herb in a Chinese patent medicine for treating heat asthma. The method comprises the following steps: a test article solution is prepared, wherein a macroporous adsorption resin column is adopted for absorption, and eluting is carried out by ethanol with the mass concentration being 35%; and a reference substance solution is prepared, wherein bergenin is taken as a control sample, a thin layer chromatography is adopted, wherein a polyamide film is used for unfolding, a mixed solution of ethyl acetate-acetic acid is taken as an unfolding agent. An experiment result is negative and free of interference. According to the method for identifying Japanese ardisia herb in the Chinese patent medicine for treating heat asthma provided by the invention, the repeatability is good, the specificity is high, spots are clear, and stable Chinese patent medicine quality can be effectively ensured.

Description

technical field [0001] The invention relates to a method for identifying components of traditional Chinese medicines, in particular to a method for identifying Chinese patent medicines for treating febrile asthma. Background technique [0002] The Chinese patent medicine for treating fever and asthma contains 160g ephedra, 360g dwarf tea, 210g scutellaria, 210g bitter almond, 170g peach kernel, 165g earthworm, 140g schisandra, 240g epimedium, 265g privet fruit, 105g licorice and granulated 1000g. The preparation method is: 265g of Ligustrum lucidum, 140g of Schisandra chinensis, heating and reflux extraction with 65% ethanol twice, each time for 1.5 hours, combining the extracts, recovering ethanol and concentrating to a relative density of 1.07-1.09 (60°C); taking another 160g of ephedra, Steam distill 210g of bitter almonds and 170g of peach kernels, and collect the distillate for later use. Dregs, 360g of Aodicha, 210g of Scutellaria baicalensis, 165g of Dilonga, 240g of...

Claims

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Application Information

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IPC IPC(8): G01N30/90
Inventor 尤金花胡永水赵婷婷牛伟霞李士栋张守元刘春兰张鲜票孙方玉
Owner SHAN DONG DONG E E JIAO
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