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Isolated antiviral natural immune protein TRIM32 (tripartite motif 32) for carps and antiviral activity

A natural immunity and anti-virus technology, applied in the direction of anti-animal/human immunoglobulin, anti-viral agent, antibody, etc.

Inactive Publication Date: 2015-05-13
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology allows scientists to study how certain proteins are involved with viruses like Japanese encephalitis by studying their ability to infect cells during different stages of its lifespan. By measuring these genes over time it becomes possible to identify which parts may be causing symptoms such as dengue fever when they were exposed to other diseases caused by this disease.

Problems solved by technology

Technical Problem: The technical problem addressed in this patented text relates to finding ways to protect against diseases like bacterial meningoencephalitis through natural immune responses called autoimmunology. Autoimic agents currently exist but their effectiveness depends heavily upon how well they work under specific conditions.

Method used

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  • Isolated antiviral natural immune protein TRIM32 (tripartite motif 32) for carps and antiviral activity
  • Isolated antiviral natural immune protein TRIM32 (tripartite motif 32) for carps and antiviral activity
  • Isolated antiviral natural immune protein TRIM32 (tripartite motif 32) for carps and antiviral activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) RNA extraction from carp

[0023] Take a female carp with a size of about 300 g, and after dissection, take mung bean-sized ovarian tissue (50-100 mg), add 1 ml of TRIzol reagent, and use a homogenizer for homogenization. The sample volume should not exceed the volume of TRIzol by 10℅.

[0024] Place the homogenized sample at room temperature (15-30° C.) for 5 minutes to completely separate the nucleic acid-protein complexes.

[0025] Centrifuge at 10,000 xg for 15 minutes at 4°C. The sample is divided into three layers: the bottom layer is a yellow organic phase, the upper layer is a colorless aqueous phase and an intermediate layer.

[0026] Transfer the aqueous phase to a new tube, add 1 / 5 of the volume of the aqueous phase in chloroform, vortex and mix, let stand on ice for 5 minutes, and centrifuge at 10,000×g at 4°C for 15 minutes.

[0027] Take the supernatant into a new centrifuge tube, add an equal volume of isopropanol, let stand on ice for 10 minutes, ...

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Abstract

The invention belongs to the technical field of aquatic animal genetic engineering, and particularly relates to an isolated antiviral natural immune protein TRIM32 (tripartite motif 32) for carps and antiviral activity. The nucleotide sequence of a gene of the antiviral natural immune protein TRIM32 for the carps is represented as 1-1986bp in SEQ ID NO: 1 and the coded amino acid sequence of the antiviral natural immune protein TRIM32 is represented as SEQ ID NO: 2. The test proves that expression of the cloned protein TRIM32 can influence proliferation of SVCV (spring viraemia of carp virus), and protein TRIM32 plays an important role in the life cycle of SVCV; the gene or the protein provides a new target for study of anti-SVCV medicine preparation.

Description

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Claims

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Application Information

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Owner HUAZHONG AGRI UNIV
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