Isolated Pantoea pineapple hyl01 strain and its application in promoting watermelon root growth
A technology of Pantoea pineapple and root growth, which is applied in the field of microorganisms, can solve the problems of phosphorus deficiency in cultivated land soil, etc., and achieve the effect of improving root system composition, improving ability, and promoting growth
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[0027] Example 1 Isolation, identification and application of Pantoea ananatis HYL01 (Pantoea ananatis HYL01) strain in promoting watermelon root growth
[0028] 1 Materials and methods
[0029] 1.1 Medium
[0030] PKO inorganic medium (1L): glucose 10.0g, (NH 4 ) 2 SO 4 0.5g, MgSO 4 ·7H 2 O 0.3g, NaCl 0.3g, KCl 0.3g, FeSO 4 ·7H 2 O 0.036g, MnSO 4 ·H 2 O 0.03g, Ca 3 (PO 4 ) 3 2.0g, 20g agar, distilled water to 1000ml, no agar added to the liquid medium.
[0031] 1.2 Purification and screening of phosphate-dissolving strains
[0032] The rhizosphere soil of gramineous plants in Qiqihar City, Heilongjiang Province was collected in March 2014. The soil on the root surface was collected by shaking the soil, put into a sterile bag, and stored in a 4°C refrigerator for later use. Weigh 1g of root mixed soil respectively, put it into a 250ml Erlenmeyer flask filled with 9ml sterile water and 8 sterile glass beads according to aseptic operation, put it in a constant temp...
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