Isolated Pantoea pineapple hyl01 strain and its application in promoting watermelon root growth

A technology of Pantoea pineapple and root growth, which is applied in the field of microorganisms, can solve the problems of phosphorus deficiency in cultivated land soil, etc., and achieve the effect of improving root system composition, improving ability, and promoting growth

Active Publication Date: 2018-04-27
QIQIHAR UNIVERSITY +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Phosphorus is one of the macronutrient elements necessary for plant growth, but there is more phosphorus in the soil than Ca 2+ , Fe 3+ , Fe 2+ 、Al 3+ Combined to form insoluble phosphate, only a small part can be absorbed and utilized by plants, which leads to the widespread phenomenon of phosphorus deficiency in cultivated soil (Taurian T, Anzuay M S, Angelini J G, et al. Phosphate-solubilizing peanut associated bacteria: screening for plant growth- promoting activities[J]. Plant and soil, 2010, 329(1-2): 421-431.)

Method used

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  • Isolated Pantoea pineapple hyl01 strain and its application in promoting watermelon root growth
  • Isolated Pantoea pineapple hyl01 strain and its application in promoting watermelon root growth
  • Isolated Pantoea pineapple hyl01 strain and its application in promoting watermelon root growth

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Embodiment 1

[0027] Example 1 Isolation, identification and application of Pantoea ananatis HYL01 (Pantoea ananatis HYL01) strain in promoting watermelon root growth

[0028] 1 Materials and methods

[0029] 1.1 Medium

[0030] PKO inorganic medium (1L): glucose 10.0g, (NH 4 ) 2 SO 4 0.5g, MgSO 4 ·7H 2 O 0.3g, NaCl 0.3g, KCl 0.3g, FeSO 4 ·7H 2 O 0.036g, MnSO 4 ·H 2 O 0.03g, Ca 3 (PO 4 ) 3 2.0g, 20g agar, distilled water to 1000ml, no agar added to the liquid medium.

[0031] 1.2 Purification and screening of phosphate-dissolving strains

[0032] The rhizosphere soil of gramineous plants in Qiqihar City, Heilongjiang Province was collected in March 2014. The soil on the root surface was collected by shaking the soil, put into a sterile bag, and stored in a 4°C refrigerator for later use. Weigh 1g of root mixed soil respectively, put it into a 250ml Erlenmeyer flask filled with 9ml sterile water and 8 sterile glass beads according to aseptic operation, put it in a constant temp...

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Abstract

The invention discloses an isolated strain of Pantoea pineapple HYL01 and its application in promoting the growth of watermelon roots. An isolated Pantoea ananatis (Pantoea ananatis) bacterial strain of the present invention, named as Pantoea ananatis HYL01, is preserved in the China Type Culture Collection Center, the address is at Wuhan University, and its microorganism preservation number is CCTCC NO.M 2014530, The date of deposit is October 29, 2014. The research results showed that the strain HYL01 had a strong ability to dissolve phosphorus, and the effective amount of dissolved Ca3(PO4)3 was 140.26mg / L. It also had the ability to secrete IAA, and the maximum secretion of IAA was 11.39mg / L. After the application of HYL01 bacterial agent, it can significantly promote the growth of total root length, root surface area, root volume and specific root length of watermelon seedlings, and significantly increase the hairy root length of watermelon seedlings in the range of root system diameter greater than 0 to less than or equal to 0.5mm Therefore, the isolated Pantoea pineapple strain HYL01 obtained by the present invention has the potential to prepare a compound microbial fertilizer that promotes the growth of watermelon roots.

Description

technical field [0001] The invention relates to a strain of phosphate-dissolving bacteria, in particular to a strain of Pantoea ananatis with auxin (IAA) synthesis ability, and also relates to the application of the strain in promoting the growth of watermelon roots. The invention belongs to the technical field of microorganisms. Background technique [0002] Phosphorus is one of the macronutrient elements necessary for plant growth, but there is more phosphorus in the soil than Ca 2+ , Fe 3+ , Fe 2+ 、Al 3+ Combined to form insoluble phosphate, only a small part can be absorbed and utilized by plants, which leads to the widespread phenomenon of phosphorus deficiency in cultivated soil (Taurian T, Anzuay M S, Angelini J G, et al. Phosphate-solubilizing peanut associated bacteria: screening for plant growth- promoting activities[J]. Plant and soil, 2010, 329(1-2): 421-431.). Soil phosphorus-dissolving microorganisms can convert fixed phosphorus that cannot be absorbed by ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 王志刚徐伟慧李珊珊胡云龙胡影刘帅王春龙才绍玉杨洋
Owner QIQIHAR UNIVERSITY
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