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SNP molecular marker for detecting porcine T cell subset character

A technology of molecular markers and cell subgroups, applied in the direction of recombinant DNA technology, microbial measurement/testing, DNA/RNA fragments, etc., can solve the problem of insufficient abundance of T cell immune-related genes

Active Publication Date: 2015-06-17
HUAZHONG AGRI UNIV
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Problems solved by technology

There are also studies involving deeper levels, such as the GWAS association study of porcine T cell subtype traits (Lu, Fu et al. CD8) quantity and its ratio, but problems such as the population used and the processing of the population caused the acquired T cell immunity-related genes to be insufficiently rich

Method used

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  • SNP molecular marker for detecting porcine T cell subset character
  • SNP molecular marker for detecting porcine T cell subset character
  • SNP molecular marker for detecting porcine T cell subset character

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Experimental program
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Embodiment Construction

[0032] 1. Sample collection

[0033]The experimental pigs came from 293 piglets of the F2 population obtained by crossing Duroc and Erhualian (obtaining the F2 population obtained by crossing Duroc and Erhualian is a conventional method, the pig breed Duroc is a breed of foreign blood, and Erhualian is a Chinese local Pig blood is a commonly used breed, and the experimental pig breed comes from the experimental pig farm of Huazhong Agricultural University). The pigs have free access to food and water, and the entire feeding method and feeding conditions are always consistent, which is a conventional method.

[0034] Blood was collected from all 35-day-old piglets within 4 hours after Poly I:C injection according to conventional methods.

[0035] 2. Determination of T cell subsets

[0036] The reagents used were mouse anti-pig CD3 monoclonal antibody, mouse anti-pig CD8 monoclonal antibody and mouse anti-pig CD4 monoclonal antibody from Southernbiotech, UK. The main instrume...

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Abstract

The invention belongs to the technical field of livestock molecular marker preparation, particularly relates to an SNP molecular marker for detecting porcine T cell subset character. The SNP molecular marker comprises an SNP molecular marker related to the T cell subset character as well as location and application of a haplotype formed by two SNPs. The SNP molecular marker provided by the invention is obtained through whole genome association analysis; the nucleotide sequence is as shown in SEQ ID NO: 1 and SEQ ID NO: 2; and an allelic mutation exists at the 26bp of the SEQ ID NO: 1 and SEQ ID NO: 2 so that polymorphism is caused. By utilizing the obtained SNP molecular marker, a corresponding haplotype as shown in figure 6 is obtained through haplotype analysis. The marker and the haplotype can be applied to the related detection of porcine T cell subset character.

Description

technical field [0001] The invention belongs to the technical field of livestock molecular marker preparation, and in particular relates to a SNP molecular marker for detecting the traits of pig T cell subsets. And the application of the haplotype formed by these two SNPs in the detection of the traits of pig T cell subsets. Background technique [0002] In recent years, in the pig breeding work, improving the disease resistance of pigs has always been an important task in the breeding work. The traditional breeding methods targeting meat quality and lean meat rate have made great contributions to the pig industry, but the disease resistance traits of pigs still need to be improved, and the frequent occurrence of epidemic virus infections has seriously affected the healthy development of the pig industry. The disease resistance of pigs has begun to attract widespread attention from farmers, governments and the scientific community. With the continuous improvement of the na...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
Inventor 赵书红陈俭海朱猛进李世军刘向东李新云曹建华李长春谢胜松
Owner HUAZHONG AGRI UNIV
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