SNP (Single Nucleotide Polymorphism) molecular marker related to stillbirth character of sow and application thereof

A technology of marking and stillbirth, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of increasing litter size and easily neglecting the survival rate of piglets

Pending Publication Date: 2022-07-08
HUAZHONG AGRI UNIV
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  • Description
  • Claims
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Problems solved by technology

However, most studies on reproductive traits in sows focus on increa

Method used

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  • SNP (Single Nucleotide Polymorphism) molecular marker related to stillbirth character of sow and application thereof
  • SNP (Single Nucleotide Polymorphism) molecular marker related to stillbirth character of sow and application thereof
  • SNP (Single Nucleotide Polymorphism) molecular marker related to stillbirth character of sow and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Genotyping detection and data processing

[0016] (1) DNA extraction

[0017] ① Collect the ear tissues of 1175 large white sows, mash the collected ear-like tissues and place them in a glass homogenizer, add equal volumes of 100 mmol / L Tris saturated phenol and 500 mmol / L EDTA Disodium (EDTA), 20 mmol / L sodium chloride (NaCl), 10% sodium dodecyl sulfate (SDS), 20 μg / ml pancreatic RNase to prepare a cell lysis buffer, and then add 10 ng / ml mL of proteinase K, mix well, and place it in a constant temperature water bath at 65°C for 30min;

[0018] Slowly shake the centrifuge tube for 15min, place it in a centrifuge at 12000rpm for 5min, and take the supernatant

[0019] into another centrifuge tube;

[0020] ②Add equal volumes of phenol, chloroform, and isoamyl alcohol (volume ratio is 25:24:1), shake and mix well, place it in a centrifuge at 12000rpm for 5min, and take the supernatant into another centrifuge tube;

[0021] ③ Add equal volumes of phenol, chl...

Embodiment 2

[0029] Example 2 Genome-wide association analysis of SNP molecular markers and stillbirth traits in large white sows

[0030] The sequence and genome-wide association analysis results in the present invention are based on the information of pig genome version 11.1

[0031] (1) Definition of phenotype

[0032] The stillbirth of a sow is regarded as a case-control trait. According to the original phenotype record of the stillbirths of each sow and each litter, the sow has two or more stillbirths as a case, and the sow has no stillbirths or only births. A stillbirth was used as a control, and case-control constructed new phenotypes for subsequent genome-wide association analysis.

[0033] (2) Quality control of SNP molecular markers

[0034] According to the reliable SNP molecular marker sites screened out after the above genotypes were filled, the PLINK v1.9 software was used to control the quality of the SNPs of the case phenotype and the control phenotype after filling, and ...

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Abstract

The invention belongs to the technical field of pig molecular markers, and particularly relates to an SNP (Single Nucleotide Polymorphism) molecular marker related to a stillbirth character of a sow and application of the SNP molecular marker. Gene typing is carried out by utilizing a gene chip technology, whole genome association analysis is carried out based on SNP molecular markers, and six SNP molecular marker sites related to the stillbirth character of the large white sow are screened out through the analysis. The sites of the SNP molecular marker are respectively positioned on SLC4A10 gene segments of the 15 # chromosome of the sow. A new SNP molecular marker resource is provided for the stillbirth character of the sow, and the SNP molecular marker can be applied to marker-assisted selection of genetic breeding of the sow.

Description

technical field [0001] The invention belongs to the technical field of swine molecular markers, and in particular relates to a SNP molecular marker related to the stillbirth trait of sows and its application. Background technique [0002] In my country, pork production and sales are at the forefront of the world. The epidemic has led to a significant decrease in the number of live pigs and the number of fertile sows. Under this situation, it is particularly important to improve the reproductive performance of sows. Reproductive traits are one of the important economic traits of sows. Molecular breeding for molecular marker sites that affect target traits can effectively improve breeding efficiency and reproductive performance of sows (Wang Yuan, 2018). In recent years, high-throughput sequencing technology and gene chip technology have developed rapidly. The development of these related technologies also serves as the premise and basis for the implementation of efficient bre...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156Y02P60/87
Inventor 马云龙董文君李望娇叶尔江·买买提吐尔干张凯丽李新云赵书红
Owner HUAZHONG AGRI UNIV
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