Molecular marker for detecting wheat fusarium blight QTL Qfsb.hbaas-6BL and application of molecular marker
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A technology for Fusarium and wheat, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., and can solve the problem of not developing reliable markers.
Pending Publication Date: 2021-11-26
INST OF FOOD CROPS HUBEI ACAD OF AGRI SCI
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The above studies all use genetic populations (DH populations) for molecular marker mapping, and no reliable ma...
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Embodiment 1
[0044] Example 1, Discovery of Qfsb.hbaas-6BL and its associated SNP markers
[0045] Test materials: natural populations formed from 240 wheat varieties (lines) at home and abroad, see Table 1. The materials used are recorded in the literature: Zhu Zhanwang, Xu Dengan, Cheng Shunhe, et al. Identification and traceability of the scab resistance gene Fhb1 in Chinese wheat varieties [J]. Acta Crops, 2018, 44(4): 473–482. The public can download from Obtained from the Institute of Food Crops, Hubei Academy of Agricultural Sciences.
[0046]Table 1 240 varieties (lines) and their sources and genotypes
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[0055] a 90K SNP chip typing results; b Mark the detection results for KASP; c Fusarium blight (FSB) lesion length (cm) for 3 repetitions, "NN" means missing chip data.
[0056] 1. Discovery of Qfsb.hbaas-6BL and its associated SNP markers
[0057] 1. Identification of Fusarium bli...
Embodiment 2
[0101] Embodiment 2, actual sample detection
[0102]Test materials: natural population varieties shown in Table 1.
[0103] 1. Identification of Fusarium blight resistance
[0104] With embodiment 1.
[0105] 2. KASP-Fsb6BL marker detection
[0106] Mark with KASP-Fsb6BL according to the method in embodiment 1, carry out KASP reaction to 240 kinds shown in table 1, detect KASP reaction product; Use PHERAstar Plus Detect the product, and use KlusterCaller software for data analysis; if the color is blue, the genotype of the wheat SNP site IWA3221 to be tested is TT; if it is green, the gene of the wheat SNP site IWA3221 to be tested is The type is CC.
[0107] Table 1 shows the marker detection results of 240 wheat cultivars and the average length of FSB lesions. The results in Table 1 were statistically analyzed to obtain the results in Table 5.
[0108] Table 5 Differences in resistance to Fusarium blight of different genotypes of KASP-Fsb6BL
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[0110] As c...
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Abstract
The invention discloses a molecular marker for detecting a wheat fusarium blight resistant QTL Qfsb.hbaas-6BL and application of the molecular marker. The invention provides application of a substance for detecting the genotype of an SNP IWA3221 site of a wheat chromosome 6BL in identification or auxiliary identification of wheat fusarium blight resistance. The invention also provides application of a substance for detecting the genotype of the SNP IWA3221 site of the wheat chromosome 6BL in preparation of a product for identification or auxiliary identification of wheat fusarium blight resistance. The fusarium blight resistant locus Qfsb.hbaas-6BL located on a wheat 1D chromosome long arm is found through whole genome association analysis (GWAS), the associated SNP IWA3221 is further converted into a common PCR marker KASP-Fsb6BL, and the typing consistency rate of the two to 240 parts of materials is 97.5%. The marker can be used for detecting the genotype of the fusarium blight resistant QTL Qfsb.hbaas-6BL, and can be used for molecular breeding of fusarium blight resistance.
Description
technical field [0001] The invention relates to the field of biotechnology, in particular to a molecular marker used for detecting QTLQfsb.hbaas-6BL resistant to wheat Fusarium blight and a method for using it. Background technique [0002] Wheat (Triticum aestivum L.) is one of the most important food crops in the world and is of great significance to food security. Wheat Fusarium seedling blight (FSB) and head blight (Fusarium headblight, FHB), mainly caused by Fusarium pathogens, are devastating diseases of wheat and other small grain crops worldwide. Wheat seedling blight can damage growing seedlings or rot them later in the growing season, resulting in reduced emergence and stunted growth, resulting in yield loss. In addition, wheat seedling blight can provide a pathogen source for subsequent wheat head blight infection, resulting in reduced wheat yield and food safety hazards. In recent years, due to changes in global climate and farming systems, wheat seedling bligh...
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