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Molecular marker related to salt tolerance of rice and application of molecular marker

A technology of molecular markers and salt tolerance, applied in the field of crop genetics and breeding, can solve the problem of insufficient mining of rice salt-tolerant molecular markers, and achieve the effect of speeding up the process

Active Publication Date: 2022-04-12
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Salt tolerance of crops is a very complex quantitative trait controlled by multiple loci, but the mining of molecular markers of salt tolerance in rice is far from enough

Method used

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  • Molecular marker related to salt tolerance of rice and application of molecular marker
  • Molecular marker related to salt tolerance of rice and application of molecular marker
  • Molecular marker related to salt tolerance of rice and application of molecular marker

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Embodiment 1

[0027] rice gene OsDSK2a The molecular marker and its application, the molecular marker is to find the rice gene by analyzing the yield data of rice core germplasm resources under salt stress stress OsDSK2a Several polymorphic sites of medium salt tolerance, including 3 SNPs and 1 INDEL. details as follows:

[0028] 1. Use the rice reference genome (os-Nipponbare-reference-IRGSp-1.0) sequence to design primers for the molecular markers.

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Abstract

The invention discloses a molecular marker related to salt tolerance of rice and application of the molecular marker. The molecular marker is obtained through whole genome association analysis, and comprises nucleotide single base mutation at the 1796216bp position, nucleotide single base mutation at the 1797072bp position, two nucleotide base mutations at the 1797131bp position and the 1797132bp position, and nine nucleotide base deletion at the 1797233bp position on the No.3 chromosome of a rice reference genome. By utilizing the molecular marker provided by the invention, the salt-tolerant genotype of the rice OsDSK2a gene can be rapidly and accurately detected, and efficient application of the gene in rice salt-tolerant molecular breeding is facilitated.

Description

technical field [0001] The invention belongs to the technical field of crop genetics and breeding, and in particular relates to a molecular marker related to rice salt tolerance. Background technique [0002] There are about 550 million mu of usable saline-alkali land in our country, which has great potential for development and is an important potential arable land resource in our country. These saline-alkali lands are mainly distributed in the inland areas of Northeast China, North China, Northwest China and the coastal areas north of the Yangtze River. Cultivating saline-alkali-resistant crop varieties and scientifically improving saline-alkali land are important prerequisites for the development and utilization of these land resources. Mining and identifying important saline-alkali-tolerant genes of crops and developing molecular markers are the most economical and effective means to accelerate the process of breeding saline-alkali-resistant crop varieties. . [0003] ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6837C12N15/11
Inventor 王娟朱睿梁宇航权瑞党秦华黄荣峰
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
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