Early diagnosis of obesity-related diseases using changes in the gut microbial community structure and function

An inflammatory disease, Brevibacterium technology, applied in the field of early diagnosis of metabolic and inflammatory diseases using changes in intestinal bacterial colonies and functions, can solve problems such as intestinal bacterial imbalance

Inactive Publication Date: 2015-06-24
KO BIOLABS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, it is known that human intestinal bacteria are unbalanced in obesity, diabetes, inflammatory bowel disease patients, etc.

Method used

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  • Early diagnosis of obesity-related diseases using changes in the gut microbial community structure and function
  • Early diagnosis of obesity-related diseases using changes in the gut microbial community structure and function
  • Early diagnosis of obesity-related diseases using changes in the gut microbial community structure and function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1. Research object and sample collection

[0060] This study was conducted on identical twins who participated in the Healthy Twin Study in Korea. The identicalness of twins is determined by the AmpFlSTR Indentifier Kit (15 autosomal short tandem repeat markers and 1 sex determination marker) and a questionnaire showing an accuracy of more than 90% The investigation confirmed that the detailed methodology of the twin generation study has been recorded in a previous paper (Sung, J. et al., Twin Research and Human Genetics, 2006.9(6): p.844-848.). Stool samples were collected once from two pairs of twins and twice from eight pairs of twins. A total of 36 fecal samples were used for metagenomic shotgun sequencing analysis. On the other hand, in order to analyze the colony structure of intestinal microorganisms, stool samples obtained from a total of 15 pairs of twins with different waist circumferences were used, and their serum samples were used to determine the cy...

Embodiment 2

[0061] Example 2. DNA extraction

[0062] Using Mobio Power Soil DNA isolation kit (Mobio Power Soil DNA isolation kit) extract all DNA from each stool sample, and keep it at -80°C until analysis.

Embodiment 3

[0063] Example 3. Shotgun sequencing of metagenomics

[0064] Using Illumina HiSeq2000 101 Cycle Paired End Sequencing, according to the manufacturer’s instructions, metagenomic shotgun sequencing was performed on the samples used in the analysis of the correlation between intestinal microorganisms and clinical medical indicators. This generated an average 3.5 gigabase (gigabase) base sequence.

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PUM

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Abstract

The present invention relates to a biomarker for risk prediction or diagnosis of metabolic and inflammatory diseases, and use thereof. The biomarker is formulated to detect a group of microorganisms consisting of Akkermansia muciniphila, Bacteroides spp., Eubacterium spp., Roseburia spp., Methanobrevibacter smithii, Methanosphaera stadtmanae, Ruminococcus obeum, and Phascolarctobacterium.

Description

Technical field [0001] The present invention relates to a composition for detecting a biomarker for predicting or diagnosing the risk of metabolic and inflammatory diseases and its use. Background technique [0002] In recent years, due to changes in the dietary life based on high-calorie diets, lack of exercise, and increased stress, the homeostasis of modern people has been disturbed. Therefore, various metabolic and inflammatory diseases are rapidly increasing. Metabolic and inflammatory diseases include not only obesity, hypertension, type 2 diabetes, inflammatory bowel disease, but also cardiovascular diseases such as stroke and arteriosclerosis, as well as various cancers, allergic diseases, atopy, etc. Serious diseases threatening human health and life. [0003] On the other hand, it is known that the colony of intestinal bacteria is affected by the host's genetic factors and environmental factors such as dietary intake. In addition, it is known that an imbalance of human ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/04
CPCC12Q1/04C12Q1/6883C12Q1/689G01N33/569C12Q2600/112
Inventor 高光杓林美泳成周宪
Owner KO BIOLABS INC
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