Composite fungal intercellular polysaccharide composition with liver protecting function and preparation method

An intracellular polysaccharide and compound bacteria technology, applied in the field of medicine, can solve the problem of not being used for liver protection, and achieve the effect of promoting the growth of liver cells, restoring liver function, and resisting liver damage.

Inactive Publication Date: 2015-07-01
JILIN MODERN TRADITIONAL CHINESE MEDICINE ENG RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Overcome the deficiency that most of the existing technologies are aimed at the extract...

Method used

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  • Composite fungal intercellular polysaccharide composition with liver protecting function and preparation method
  • Composite fungal intercellular polysaccharide composition with liver protecting function and preparation method
  • Composite fungal intercellular polysaccharide composition with liver protecting function and preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0021] Take Coprinus pilosula, Tremella, and Huangling mushrooms and go through the same steps as follows:

[0022] Fermenting and culturing, separating after fermentation, drying the obtained mycelia, grinding and pulverizing the dried mycelium, and passing through a 60-mesh sieve. Add 3 times the volume of distilled water, adjust the pH to 7.0, soak for 40 minutes, keep the temperature at 55°C, add 0.5% cellulase, mix for 2 hours, centrifuge for 20 minutes, add 0.3% pectinase solution for precipitation, and mix for 1.5 h, centrifuge for 20 minutes, and finally add 0.5% protease to the pellet for 1 hour each. Ultrasonic: (1) add distilled water to the mycelium powder after compound enzymolysis, the ratio of solid to liquid is 1:50, and treat it under ultrasonic conditions with a power of 700W for 60min; repeat the above operation twice, extract with hot water at 80°C for 2h, and filter with suction , concentrated to 1 / 5 of the original volume, then add 3 times the volume of ...

Embodiment 2

[0025] Take Coprinus pilosula, Tremella, and Huangling mushrooms and go through the same steps as follows:

[0026] Fermenting and culturing, separating after fermentation, drying the obtained mycelia, grinding and pulverizing the dried mycelium, and passing through a 60-mesh sieve. Add 4 times the volume of distilled water, adjust the pH to 7.0, soak for 60 minutes, keep the temperature at 55°C, add 0.5% cellulase, mix for 2 hours, centrifuge for 20 minutes, add 0.3% pectinase solution for precipitation, and mix for 1.5 h, centrifuge for 20 minutes, and finally add 0.5% protease to the pellet for 1 hour each. Ultrasonic: (1) add distilled water to the mycelium powder after compound enzymolysis, the ratio of solid to liquid is 1:40, and treat it under ultrasonic conditions with a power of 700W for 60min; repeat the above operation twice, extract with hot water at 80°C for 2h, and filter with suction , concentrated to 1 / 5 of the original volume, then add 5 times the volume of ...

Embodiment 3

[0029] Take Coprinus pilosula, Tremella, and Huangling mushrooms and go through the same steps as follows:

[0030] Fermenting and culturing, separating after fermentation, drying the obtained mycelia, grinding and pulverizing the dried mycelium, and passing through a 60-mesh sieve. Add 5 times the volume of distilled water, adjust the pH to 7.0, soak for 60 minutes, keep the temperature at 55°C, add 0.5% cellulase, mix for 2 hours, centrifuge for 20 minutes, add 0.3% pectinase solution for precipitation, and mix for 1.5 h, centrifuge for 20 minutes, and finally add 0.5% protease to the pellet for 1 hour each. Ultrasonic: (1) add distilled water to the mycelium powder after compound enzymolysis, the ratio of solid to liquid is 1:80, and treat it under ultrasonic conditions with a power of 700W for 60min; repeat the above operation twice, extract with hot water at 80°C for 2h, and filter with suction , concentrated to 1 / 5 of the original volume, then add 3 times the volume of ...

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Abstract

The invention discloses a composite fungal intercellular polysaccharide composition with a liver protecting function and a preparation method. The composite fungal intercellular polysaccharide composition comprises 10-45 weight parts of coprinus comatus intercellular polysaccharide, 20-50 weight parts of white fungus intercellular polysaccharide and 20-45 weight parts of hohenbuehelia serotina intercellular polysaccharide. The preparation method comprises the steps of fermenting fungi, crushing the separated mycelia, adding enzyme for reacting, and separating solid and liquid phases; adding distilled water into the solid phase, then performing ultrasonic treatment, adding ethanol liquid, collecting and drying the sediment; concentrating the liquid phase, adding ethanol, standing, centrifuging to separate the sediment; combining the sediments obtained by solid and liquid phase treatment to obtain homogenous intercellular polysaccharides; and mixing the coprinus comatus intercellular polysaccharide, the white fungus intercellular polysaccharide and the hohenbuehelia serotina intercellular polysaccharide obtained by the method respectively to obtain the composite fungal intercellular polysaccharide composition with the liver protecting function. The composition has the advantages of effectively reducing the ALT (alanine aminotransferase) and AST (aspartate aminotransferase) level of serum, obviously reducing the malonaldehyde content of injured liver and obviously promoting biliary excretion so that the function of the injured liver can be recovered.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to a composite bacterial intracellular polysaccharide composition with hepatoprotective effect. Background technique [0002] Fungal polysaccharides are a type of active polysaccharides isolated from fungal fruiting bodies, mycelia, and fermentation broths that can control cell division and differentiation, regulate cell growth, and aging. Generally, they are composed of monosaccharides with more than ten molecules through glycosidic bonds. Linked polymers. A large number of β(1-3) and β(1-6) glycosidic bonds are combined between fungal polysaccharide molecular monomers to form chain molecules with a helical three-dimensional configuration. Intracellular polysaccharides of fungi are polysaccharides that exist inside the cells and in the mycelium of fungi, and have high activity. A large number of scientific experiments have shown that polysaccharides in fungi have various activi...

Claims

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Application Information

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IPC IPC(8): A61K36/07A61P1/16
Inventor 赵俞白冰左柏徐建徐云任晶侯宝朱丽佳
Owner JILIN MODERN TRADITIONAL CHINESE MEDICINE ENG RES CENT
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