Method of inducing hymphomycetes to spore

A technology of hyphosporium fungi and strains, applied in the field of microorganisms, can solve the problems of less sporulation, ineffective effects, and differences in conidia characteristics, and achieve the effects of mild culture conditions, avoid repeated culture, and reduce workload

Active Publication Date: 2015-07-01
WEIFANG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, under the existing culture technology, in the process of isolation and cultivation of hyphosporium fungi, it is easy to produce conidia or difficult to produce conidia during artificial culture, which makes it impossible to carry out the identification research of hyphosporium fungi
At the same time, the observation of sporulation phenotype cannot be carried out at the same time as the production of conidia, and multiple re-cultivation is often required to observe the sporulation phenotype, which consumes a lot of time and experimental costs
[0004] At present, the main methods for promoting hyphosporium sporulation include colony scratching method, ultraviolet light ir

Method used

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  • Method of inducing hymphomycetes to spore
  • Method of inducing hymphomycetes to spore
  • Method of inducing hymphomycetes to spore

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Take a circular filter paper, cut out 4 filter paper holes evenly on the filter paper, place it flat on a petri dish, add an appropriate amount of distilled water to soak the filter paper, and keep it sterilized at a temperature of 121°C and a pressure of 120kPa for 20 minutes. , remove the filter paper.

[0034] To prepare agar medium, sterilize the prepared agar medium at a temperature of 121°C and a pressure of 120kPa, then add it to the sterilized petri dish, and the amount of each petri dish is 16ml. After solidification, agar culture plates were made.

[0035] Spread the sterilized filter paper on the surface of the agar culture plate with sterile tweezers, so that there is no gap or bubble between the filter paper and the agar culture plate.

[0036] Then use a sterile needle to pick an appropriate amount of non-sporogenous hyphomycete fungi, and inoculate them on the modified agar culture plate at the center of each filter paper hole on the filter paper.

[00...

Embodiment 2

[0039] Take a circular filter paper with a diameter of 70mm, cut out 5 filter paper holes with a diameter of 15mm evenly on the filter paper, place it flat in a petri dish with a diameter of 90mm, add an appropriate amount of distilled water to soak the filter paper, and heat the filter paper at a temperature of 121 After maintaining the sterilization for 20 minutes under the condition of ℃ and pressure of 120kPa, take out the filter paper.

[0040] Add 15g of agar powder, 8g of glucose, 8g of starch, 4g of sodium carboxymethylcellulose, 8g of peptone, 3g of magnesium sulfate, 3g of potassium dihydrogen phosphate, 4g of ferrous sulfate, and 75mg of vitamin B in 1000ml of water 1 , stir evenly, and prepare agar medium, sterilize the prepared agar medium at a temperature of 121°C and a pressure of 120kPa, then add it to the sterilized petri dish, and the amount of each petri dish is 18ml. After the agar medium is solidified, make an agar culture plate.

[0041] Spread the steri...

Embodiment 3

[0046] Take a circular filter paper with a diameter of 70mm, cut out 6 filter paper holes with a diameter of 12mm evenly on the filter paper, place it flat in a petri dish with a diameter of 90mm, add an appropriate amount of distilled water to soak the filter paper, at a temperature of 121 After maintaining the sterilization for 20 minutes under the condition of ℃ and pressure of 120kPa, take out the filter paper.

[0047] Add 18g of agar powder, 6g of glucose, 6g of starch, 4g of sodium carboxymethylcellulose, 7g of peptone, 4g of magnesium sulfate, 4g of potassium dihydrogen phosphate, 3g of ferrous sulfate and 70mg of vitamin B in 1000ml of water 1 , stir evenly, and prepare agar medium, sterilize the prepared agar medium at a temperature of 121°C and a pressure of 120kPa, then add it to the sterilized petri dish, and the amount of each petri dish is 16ml. After the agar medium is solidified, make an agar culture plate.

[0048] Spread the sterilized filter paper on the s...

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Abstract

The invention discloses a method of inducing hymphomycetes to spore. The method comprises the following steps: (1) circular filter paper is taken, more than four filter paper holes are formed by uniformly shearing the filter paper, the filter paper is flatly placed in culture dishes and is soaked by adding a proper amount of distilled water, and the filter paper is taken out after high temperature and high pressure sterilization; (2) an agar medium is prepared and is sterilized, then the agar medium is added into the sterilized culture dishes, the addition amount of the agar medium in each culture dish is 15-20ml, and agar culture plates are manufactured after the agar culture medium is solidified; (3) the sterilized filter paper is flatly paved on the surface of each agar culture plate; (4) a proper number of hymphomycetes strains which do not spore are selected and are inoculated on the improved agar culture plate at the center position of each filter paper hole; (5) culture dish covers cover and are sealed, and the culture dishes are cultured for 10-30 days at room temperature under natural light rays. The method is simple to operate, the culture conditions are mild, the effect of inducing the hymphomycetes to spore is obvious, the method is effective for most hymphomycetes, conidiums are uniformly distributed on the filter paper, and sporing phenotypes are convenient to observe.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method for inducing sporulation of hyphosporium fungi. Background technique [0002] For a long time, the classification and identification of hyphomycetes have been mainly based on the morphological characteristics of the fungi, and the characteristics of conidia (shape, size, number of septa, surface decoration and appendages, etc.) are the main species-level classification criteria for hyphomycetes. The continuous appearance of conidia (sporulation phenotype) is also an important basis for the identification of hyphosporium species. [0003] However, under the existing culture technology, in the process of isolation and cultivation of hyphosporium fungi, it is easy to produce conidia or difficult to produce conidia during artificial culture, which makes it impossible to carry out the identification research of hyphosporium fungi smoothly. At the same time, the observ...

Claims

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Application Information

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IPC IPC(8): C12N3/00
Inventor 潘好芹夏海波赵静李艳青
Owner WEIFANG UNIV OF SCI & TECH
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