Nitzschia closterium culture medium

A technology of Nitzschia and culture medium, applied in the direction of single-cell algae, microorganism-based methods, microorganisms, etc., can solve the problems of low biomass, unfavorable large-scale cultivation, slow growth, etc., and achieve high cell density and suitable for large batches Large-scale culture production, the effect of increasing the concentration of algae cells

Inactive Publication Date: 2015-07-08
王兆伟
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main problems in the large-scale cultivation of Nitzschia mino

Method used

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  • Nitzschia closterium culture medium

Examples

Experimental program
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Effect test

Embodiment 1

[0010] (1) Use natural seawater to configure the following formula of Nitzschia microcystis culture medium: NaNO 3 80mg / L, NaH 2 PO 4 8mg / L, ferric ammonium citrate 2.5mg / L, Na 2 EDTA12mg / L, Na 2 SiO 3 9H 2 O20mg / L, diphenylurea 0.1mg / L, p-chlorophenoxyacetic acid 0.1mg / L, VB 1 0.006mg / L, VB 12 0.05mg / L, ZnSO 4 4H 2 O23μg / L, MnCl 2 4H 2 O178μg / L, CuSO 4 ·5H 2 O10μg / L, Na 2 MoO 4 2H 2 O7.3μg / L and CoCl 2 ·6H 2 O12μg / L.

[0011] (2) Inoculate Nitzschia smalle in the logarithmic growth phase into the medium for cultivation, light for 10 hours every day, and the light intensity is about 50 μmol / (m 2 s), the temperature of the incubator was controlled at 20 degrees Celsius, and the cell concentration of Nitzschia lunster in the medium was recorded at regular intervals, see Table 1 for details.

Embodiment 2

[0013] (1) Use natural seawater to configure Nannochloropsis medium with the following formula: NaNO 3 120mg / L, NaH 2 PO 4 12mg / L, ferric ammonium citrate 3.5mg / L, Na 2 EDTA12mg / L, Na 2 SiO 3 9H 2 O30mg / L, diphenylurea 1mg / L, p-chlorophenoxyacetic acid 1mg / LVB 1 0.006mg / L, VB 12 0.05mg / L, ZnSO 4 4H 2 O23μg / L, MnCl 2 4H 2 O178μg / L, CuSO 4 ·5H 2 O10μg / L, Na 2 MoO 4 2H 2 O7.3μg / L and CoCl 2 ·6H 2 O12μg / L.

[0014] (2) Inoculate Nitzschia smalle in the logarithmic growth phase into the medium for cultivation, light for 10 hours every day, and the light intensity is about 50 μmol / (m 2 s), the temperature of the incubator was controlled at 20 degrees Celsius, and the cell concentration of Nitzschia lunster in the medium was recorded at regular intervals, see Table 1 for details.

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Abstract

The invention relates to a nitzschia closterium culture medium comprising the following components, by mass concentration: 80-120mg/L of NaNO3, 8-12mg/L of NaH2PO4, 2.5-3.5mg/L of ferric ammonium citrate, 10-12mg/L of Na2EDTA, 20-30mg/L of Na2SiO3.9H2O, 0.005-0.007mg/L of VB1, 0.04-0.06mg/L of VB12, 22-24mu g/L of ZnSO4.4H2O, 175-180mu g/L of MnCl2.4H2O, 9-11mu g/L of CuSO4.5H2O, 7.0-7.5mu g/L of Na2MoO4.2H2O, 11-13mu g/L of CoCl2.6H2O, and a plant growth regulator comprising 0.1-1mg/L of diphenyl urea and 0.1-1mg/L of p-chlorophenoxyacetic acid. When the culture medium is used for culturing nitzschia closterium, advantages of fast growth speed and high cell density are shown. The culture medium is suitable for large-scale productions.

Description

technical field [0001] The invention relates to an algae culture medium, in particular to a culture medium for Nitzkiris small. Background technique [0002] Small Crescent Nitzophrenia belongs to Diatomphyta, Plumenaea, Dirhomboides, Nitzphyceae, Nitzphylla, the center of the cell expands into a spindle shape, and the two ends are acuminate, like a crescent. The cells are 12-23 μm long and 2-3 μm wide, with a nucleus in the center. Chromosome yellowish brown, two pieces, located on both sides of nucleus. Distributed in seawater, brackish and freshwater waters of the world, it occurs in every sea area of ​​my country. The optimum salinity range is 25-32%, the optimum temperature is 15-20°C, and the growth will stop if the water temperature exceeds 28°C; the optimum light intensity is 3000-8000lx, avoid direct sunlight during cultivation; the optimum pH is 7-10, and the optimum is 7.5-8.5 . Small crescent algae, commonly known as "small diatoms", is rich in protein, carbo...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 王兆伟
Owner 王兆伟
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