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Prevention and control of Aspergillus flavus and toxins in crop storage period Shewanella algae and its application

A technology for Shewanella and Aspergillus flavus is applied to the seaweed Shewanella for preventing and controlling Aspergillus flavus and toxins in crop storage period and its application field, which can solve problems such as difficulty in preventing and controlling aflatoxins, and achieve the goal of inhibiting the incidence of Aspergillus flavus. It has the effect of simple synthesis and cultivation of toxins and obvious bacteriostatic effect.

Inactive Publication Date: 2018-04-10
HUAZHONG AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to carry out research on the difficulty of preventing and controlling Aspergillus flavus and aflatoxin during the storage period of crops such as peanuts and corn, serious pollution and economic losses, and to use microbiological methods to isolate and identify a strain that can effectively prevent and control Aspergillus flavus and toxin pollution. Shewanella strain YM8 can effectively inhibit the infection of Aspergillus flavus and the production of toxins on crops such as peanuts and corn by using the small molecule volatile metabolites with antibacterial effect produced by the algae Shewanella strain on the medium

Method used

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  • Prevention and control of Aspergillus flavus and toxins in crop storage period Shewanella algae and its application
  • Prevention and control of Aspergillus flavus and toxins in crop storage period Shewanella algae and its application
  • Prevention and control of Aspergillus flavus and toxins in crop storage period Shewanella algae and its application

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Embodiment 1

[0031] Isolation and bacteriological identification of the candidate bacterial strain YM8 of embodiment 1

[0032] (1) Isolation of candidate strain YM8

[0033] Using microbiological methods, we collected marine sediments from the Bohai Sea in Dalian City, Liaoning Province, China, and isolated a strain of algal Shewanella strain with high antibacterial effect on Aspergillus flavus. We numbered the candidate strain YM8.

[0034] (2) Identification of morphological characteristics of YM8 strain

[0035] After YM8 strain was cultured in NA medium (beef extract 3.0g / L, peptone 10.0g / L, NaCl 5.0g / L, agar 15.0g / L, supplemented with distilled water to 1L; adjust pH to 7.2), the visible colonies were pale Yellow, the surface of the colony is smooth, the edges are neat, opaque, and the bacterial liquid is viscous (see figure 1 ). Gram stain was negative. Electron microscopy showed that the bacteria were short rod-shaped (see figure 2 ). The biochemical reaction proves that the...

Embodiment 2

[0043] The bacteriostasis of embodiment 2 seaweed Shewanella YM8 to Aspergillus flavus

[0044] Screening of the inhibitory effect of the strain YM8 on the growth of Aspergillus flavus hyphae was carried out in the way of buckling the Petri dish (diameter 9cm). Among them, Aspergillus flavus was cultured in PDB medium (200.0 g of peeled potatoes boiled in water for 20 minutes, then filtered through gauze to collect the filtrate, added 20.0 g of glucose, and distilled water was adjusted to 1 L, natural pH, that is, the pH was not adjusted), and after three days of cultivation, the white fungus was picked. Silk clusters were inoculated in the center of a Petri dish containing PDA. Strain YM8 (100 μl, 10 8 cfu / ml) spread on the surface of another culture dish containing NA medium. Buckle the culture dish inoculated with Aspergillus flavus mycelia on the culture dish coated with YM8, and seal it with scotch tape for preservation. The treatment inoculated only with Aspergillus f...

Embodiment 3

[0051] Embodiment 3 active carbon is to the influence of algal Shewanella YM8 bacteriostasis

[0052] In view of activated carbon as an adsorbent for volatile substances, we added activated carbon to the co-cultivation experiment of strain YM8 with Aspergillus flavus. The method is to add 5 g of activated carbon to one end of a 9 cm petri dish with a separation in the middle, and coat the other end with YM8 bacterial solution (50 μl, 10 8 cfu / ml), the mycelium block of Aspergillus flavus was inoculated in the center of another petri dish containing PDA medium. Put the petri dish inoculated with Aspergillus flavus mycelium upside down on the petri dish added with activated carbon and YM8. Four treatments as follows were adopted in the test: (1): Aspergillus flavus mycelium block; (2) Aspergillus flavus mycelium block+activated carbon; (3) Aspergillus flavus mycelium block+YM8; (4) Aspergillus flavus mycelium block+YM8; Block + activated carbon + YM8. Three replicates were se...

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Abstract

The invention belongs to the technical field of biological control of plant diseases, and in particular relates to Shewanella algae for preventing and controlling Aspergillus flavus and toxins in the storage period of crops and an application thereof. The present invention includes the identification of the seaweed Shewanella algae (Shewanella algae) YM8, and the antibacterial effect in a broad-spectrum and wide-temperature range, and at the same time, the antibacterial gas metabolites produced by the strain are identified, and the antibacterial activity is carried out. analysis etc. The isolated seaweed Shewanella YM8 of the present invention is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC NO: M2015120. The present invention also detects the application of the bacterial strain YM8 in inhibiting peanut and corn flavus diseases and toxin pollution under storage environments with different water activities.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases, and specifically relates to a strain of Shewanella algae for preventing and controlling the infection of Aspergillus flavus and the production of toxins during storage and its application. The invention also relates to the screening and identification of the Shewanella algae YM8 , broad-spectrum antibacterial effect, antibacterial gas metabolite detection and activity analysis, etc. At the same time, the application of this strain in inhibiting peanut and corn flavus diseases and toxin pollution under storage conditions with different water activities was verified. Background technique [0002] Aspergillus flavus is a widely distributed saprophytic fungus. According to the sexual stage, it is divided into Ascomycota, Eurotiales, Trichocomaceae and Petromyces. The optimum water activity for the growth of Aspergillus flavus is 0.86–0.96, the optimum temperature is 37°C...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12Q1/18A23B9/28C12R1/01
CPCA23B9/28C12Q1/18C12N1/205C12R2001/01
Inventor 廖玉才宫安东李和平张静柏
Owner HUAZHONG AGRI UNIV
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