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Immune globulin G immunoturbidimetry detection kit

A technology for immunoglobulin and detection kits, which is applied in the field of immunoglobulin IgG immunoturbidimetric detection kits, can solve the problems of low accuracy and sensitivity, poor stability, and limited popularization and application, and achieve enhanced stability performance and improved analytical sensitivity

Inactive Publication Date: 2015-07-15
BIOBASE BIODUSTRY (SHANDONG) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, common immunoglobulin IgG immunoturbidimetric assay reagents are not stable, and their accuracy and sensitivity are not high, which limits their clinical application.

Method used

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  • Immune globulin G immunoturbidimetry detection kit
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  • Immune globulin G immunoturbidimetry detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A conventional detection kit for immunoglobulin IgG immunoturbidimetric method, which comprises reagent R1, reagent R2 and calibrator.

[0025] Wherein the reagent R1 consists of:

[0026] pH 7.6 Tris buffer 18.16 mmol / L

[0027] Sodium chloride 123.20 mmol / L

[0028] Polyethylene glycol 6000 6%

[0029] Reagent R2 consists of:

[0030] Tris buffer pH 7.6 18.16 mmol / L

[0031] Goat anti-human IgG antibody 30ml / L

[0032] The test kit described in this embodiment, when in use, its assay method is to adopt the Toshiba 120 automatic analyzer with double reagent function, operate as follows:

[0033] Add 2 μl of physiological saline, sample or calibrator, then add 250 μl of R1 reagent for pre-incubation for 5 minutes and read the absorbance A1, then add 50 μl of reagent R2 and react for 5 minutes, read the absorbance A2, and calculate ΔA.

[0034] The calibrator used in this example is the IgG calibrator produced by Jiufeng Runda Biotechnology Co., Ltd.

Embodiment 2

[0036] An immunoglobulin IgG immunoturbidimetric detection kit comprises reagent R1, reagent R2 and calibrator.

[0037] Wherein the reagent R1 consists of:

[0038] pH 7.6 Tris buffer 18.16 mmol / L

[0039] Sodium chloride 123.20 mmol / L

[0040] Polyethylene glycol 6000 6%

[0041] Silica coated magnetic nanoparticles 0.1%

[0042] Kathon-CG 0.05%

[0043] Reagent R2 consists of:

[0044] Tris buffer pH 7.6 18.16 mmol / L

[0045]Goat anti-human IgG antibody 30ml / L

[0046] Bovine serum albumin 2g / L

[0047] Kathon-CG 0.05%

[0048] Concrete determination method is with embodiment 1.

Embodiment 3

[0050] An immunoglobulin IgG immunoturbidimetric detection kit comprises reagent R1, reagent R2 and calibrator.

[0051] Wherein the reagent R1 consists of:

[0052] pH 7.6 Tris buffer 18.16 mmol / L

[0053] Sodium chloride 123.20 mmol / L

[0054] Polyethylene glycol 6000 6%

[0055] Silica coated magnetic nanoparticles 1%

[0056] Kathon-CG 0.05%

[0057] Reagent R2 consists of:

[0058] Tris buffer pH 7.6 18.16 mmol / L

[0059] Goat anti-human IgG antibody 30ml / L

[0060] Bovine serum albumin 2g / L

[0061] Kathon-CG 0.05%

[0062] Concrete determination method is with embodiment 1.

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Abstract

The invention discloses an Immune globulin G immunoturbidimetry detection kit and belongs to the technical field of clinical in vitro detection reagents. The detection kit comprises a reagent R1, a reagent R2 and a calibrator. A certain amount of magnetic nano-particles covered with silicone dioxide is added to the reagent R1, a certain amount of bovine serum albumin and a certain amount of Kathon-CG are added to the reagent R2, and therefore the stability and the analysis sensitivity of the detection kit are effectively improved; the linear range of the detection kit is good, the accuracy of the reagents is high, and the detection kit can be further applied and popularized on the market.

Description

technical field [0001] The invention relates to the technical field of clinical in vitro detection reagents, in particular to an immunoglobulin IgG immunoturbidimetric detection kit. Background technique [0002] Immunoglobulins are composed of heavy chains and light chains. According to the chemical structure of the constant region of the heavy chain (H chain), the corresponding immunoglobulins are divided into various types, namely immunoglobulin M (IgM), Immunoglobulin D (IgD), Immunoglobulin A (IgA), Immunoglobulin E (IgE), and Immunoglobulin G (IgG). [0003] IgG is the abbreviation of immunoglobulin G (Immunoglobulin G, IgG), which is the main antibody component of serum, accounting for about 75% of serum Ig. Among them, 40-50% are distributed in serum, and the rest are distributed in tissues. IgG is the only immunoglobulin that can cross the placenta. The function of IgG mainly plays a protective role in the body's immunity, most of which are antibacterial and anti...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6854
Inventor 甘宜梧董雯谭柏清李静王绮
Owner BIOBASE BIODUSTRY (SHANDONG) CO LTD
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