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A method for measuring sugar content in Morinda officinalis

A technology of Tiannaisi and Naisitang, which is applied in the field of medicine, can solve the problems of complex sample processing in determination methods

Active Publication Date: 2017-01-11
广州浩意万医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the current determination methods require expensive and sophisticated instruments and complicated sample processing processes.

Method used

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  • A method for measuring sugar content in Morinda officinalis
  • A method for measuring sugar content in Morinda officinalis
  • A method for measuring sugar content in Morinda officinalis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0106] (1) Preparation of reference substance solution: take about 15 mg of Naisisose reference substance, accurately weigh it, place it in a 10mL volumetric flask, add 60% ethanol to dissolve and dilute to the mark, shake well to obtain 1.586mg of Nasisose per mL stock solution of the reference substance. Precisely draw an appropriate amount and dilute with 60% ethanol to prepare reference substance solutions at other concentration points.

[0107] Preparation of the test solution: take Morinda officinalis medicinal material (batch number: 100201) and put it in a drying oven at 60°C overnight, crush it and pass it through a 20-mesh sieve, take about 0.5g, weigh it accurately, put it in a conical flask, add 20mL 60% ethanol, ultrasonic (80kHz) extraction for 20min, filtration, filter residue washed with a little 60% ethanol, combined filtrate and dilute to 25mL, shake well, pass through 0.45μm microporous membrane, take the subsequent filtrate to prepare the test solution . ...

Embodiment 2

[0112] (1) Preparation of the reference substance solution: take about 15 mg of Nasis sugar reference substance, accurately weigh it, place it in a 10 mL volumetric flask, add water to dissolve and dilute to the mark, and shake well to prepare a reference substance containing 1.586 mg of Nass sugar per mL stock solution. Precisely draw an appropriate amount and dilute with water to prepare reference substance solutions at other concentration points.

[0113] Preparation of the test solution: take Morinda officinalis medicinal material (batch number: 100201) and put it in a drying oven at 60°C overnight, crush it and pass it through a 20-mesh sieve, take about 0.5g, weigh it accurately, put it in a conical flask, add 20mL Water, ultrasonic (80kHz) extraction for 20min, filter, wash the filter residue with a little water, combine the filtrate and dilute to 25mL, shake well, pass through a 0.45μm microporous membrane, take the subsequent filtrate to prepare the test solution.

...

Embodiment 3

[0118] (1) Preparation of the reference substance solution: take about 15 mg of Nasis sugar reference substance, accurately weigh it, place it in a 10 mL volumetric flask, add water to dissolve and dilute to the mark, and shake well to prepare a reference substance containing 1.586 mg of Nass sugar per mL stock solution. Precisely draw an appropriate amount and dilute with water to prepare reference substance solutions at other concentration points.

[0119] Preparation of the test solution: take Morinda officinalis medicinal material (batch number: 100201) and put it in a drying oven at 60°C overnight, crush it and pass it through a 20-mesh sieve, take about 0.5g, weigh it accurately, put it in a conical flask, add 20mL Water, ultrasonic (80kHz) extraction for 20min, filter, wash the filter residue with a little water, combine the filtrate and dilute to 25mL, shake well, pass through a 0.45μm microporous membrane, take the subsequent filtrate to prepare the test solution.

...

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Abstract

The invention provides a method for determining the nystose content of morinda officinalis roots by scanning a thin-layer image through software. According to the method, a trapezoidal integral method is adopted for integration of chromatographic peaks, and quantitative analysis is performed according to the relation between the peak area and the sample concentration. The method is simple to operate and can substitute a traditional thin layer scanner.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a thin-layer quantitative detection method of Morinda officinalis naissin. Background technique [0002] Morinda officinalis How, a traditional Chinese medicine, is the dry root of the dicotyledonous plant Morinda officinalis How. Morinda officinalis medicine and food have the same origin, and "Shen Nong's Materia Medica" classifies it as top grade, and it is also one of the famous "Four Southern Medicines" in China. Sweet, pungent, slightly warm in nature, returns to the kidney and liver meridian, has the functions of nourishing kidney yang, strengthening muscles and bones, and dispelling rheumatism. Soft etc. [0003] Chemical studies on Morinda officinalis in recent years have shown that it contains anthraquinone, iridoids and their glycosides, organic acids, sugars and other components. Among them, oligosaccharides occupying an absolute content advantage are the main active ingredi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90G01N30/94
Inventor 孙帅许舜军杨柳许艺镌许铮弟张和灿
Owner 广州浩意万医药科技有限公司
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