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Preparing method of sodium aescinate, external use preparation comprising same and application thereof

A escin sodium and external preparation technology, applied in the field of medicine and biology, can solve the problems of environmental pollution and high cost, and achieve the effects of overcoming environmental pollution problems, simple crystallization, and reducing usage

Active Publication Date: 2015-07-29
西安蓝绿卓生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] In order to solve the above problems, the present invention provides a method for preparing sodium aescin with simple process, only extracting with water or alcohol, purifying and selecting an aqueous solution of inorganic salt as mobile phase ion exchange chromatography (chromatography), enriching aescin , so that the elution volume is reduced, the later operation is simple and time-saving, the cost is reduced, and the problems of high cost and environmental pollution in the traditional preparation method of sodium aescinate are solved, which is in line with the development trend of preparing natural, safe and high-purity aescin

Method used

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  • Preparing method of sodium aescinate, external use preparation comprising same and application thereof
  • Preparing method of sodium aescinate, external use preparation comprising same and application thereof
  • Preparing method of sodium aescinate, external use preparation comprising same and application thereof

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Embodiment 1

[0050] The preparation of embodiment 1. sodium aescinate:

[0051] Weigh 500g of Sala seeds, pulverize, soak its powder with 20% ethanol solution of 2500ml for 1 hour, and extract by conventional reflux for 1 hour, control the temperature at 75°C, filter, and leave the filtrate; add 1500ml of 10% ethanol solution to the filter residue, Conventional reflux extraction for 1 hour, temperature controlled at 75°C, filtration, and the filtrate was retained; 1000ml of 5% ethanol solution was added to the filter residue, conventional reflux extraction for 0.5 hour, temperature controlled at 80°C, filtration; discard the residue. Combine three filtrates, a total of 3900ml. Add activated carbon to the filtrate while stirring until the brown-red color disappears, let it stand for 20 minutes, centrifuge the decolorized solution at 3500rpm for 15 minutes to remove the precipitate, concentrate the supernatant by rotary evaporation to recover ethanol, and filter the concentrated solution wit...

Embodiment 2

[0063] The preparation of embodiment 2. sodium aescinate:

[0064]Weigh 500g of Sala seeds, pulverize, soak its powder with 2500ml of 50% ethanol solution for 1 hour, extract under conventional reflux for 1 hour, control the temperature at 70°C, filter, and leave the filtrate; add 1500ml of 40% ethanol solution to the filter residue, Conventional reflux extraction for 1 hour, the temperature was controlled at 70°C, filtered, and the filtrate was retained; 1000ml of 30% ethanol solution was added to the filter residue, conventional reflux extraction was performed for 0.5 hours, the temperature was controlled at 70°C, and the filter was discarded. Combine three filtrates, a total of 3500ml.

[0065] Add active carbon to the filtrate while stirring until the brown-red color disappears, let it stand for 20 minutes, filter the decolorized solution with suction, remove the precipitate, concentrate the filtrate with rotary evaporation to recover ethanol, and filter the concentrated s...

Embodiment 3

[0071] The preparation of embodiment 3. sodium aescinate:

[0072] Weigh 500g of Sala seeds, pulverize, soak its powder with 2500ml of 80% ethanol solution for 1 hour, extract under conventional reflux for 1 hour, control the temperature at 70°C, filter, and leave the filtrate; add 2000ml of 50% ethanol solution to the filter residue, Conventional reflux extraction for 1 hour, the temperature was controlled at 70°C, filtered, and the residue was discarded. Combine two filtrates, a total of 3000ml.

[0073] Add activated carbon to the filtrate while stirring until the brown-red color disappears, let it stand for 20 minutes, filter the decolorized solution with suction, remove the precipitate, concentrate the filtrate with rotary evaporation to recover ethanol, and filter the concentrated solution with a 0.45 μm membrane to obtain 940ml of filtrate. spare.

[0074] SP-Sepharose F.F ion-exchange chromatography column and chromatographic conditions are the same as in Example 1. ...

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Abstract

The invention relates to a preparing method of sodium aescinate, an external use preparation comprising the sodium aescinate,and application thereof, in particular to the preparing method of the sodium aescinate, preparation of the external use preparation and application to the preparation of medicine for preventing or treating tissue edema due to trauma. The preparing method is characterized by comprising extraction and separation and purification steps, wherein for the extraction, buckeye powder is extracted by 0-80-percent of ethanol or methanol solution (preferably methanol solution), the separation and purification step comprises the ion exchange chromatography by using inorganic sodium salt water solution as a flowing phase. The method has the advantages that the purity of prepared products is high, the production period is short, the cost is low, the problems such as environment pollution are avoided, and the like, and the method conforms to the development trends of preparing natural safe and high-purity aescin.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and relates to a preparation method of sodium aescinate, an external preparation containing the same and an application thereof. Specifically, the present invention relates to the preparation technology of extracting sodium aescinate from the seeds of the traditional Chinese medicine Su Luo and the technology of preparing the sodium aescinate prepared by this technology into an external preparation drug (aerosol / spray), and applying it externally The preparation is applied to the treatment of injuries such as tissue edema caused by trauma. Background technique [0002] Minor injuries to the human body, including various contusions, abrasions, sprains, etc., often cause edema, pain, and limited movement in the injured area of ​​the patient, which brings inconvenience to life and work, and some may even cause serious consequences due to improper post-injury treatment. Injury within 24...

Claims

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Application Information

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IPC IPC(8): C07J63/00C07H15/256C07H1/08A61K31/704A61P7/10A61P29/00A61P23/00A61K31/166
CPCA61K9/0014A61K9/12C07J63/008
Inventor 王增禄王多宁于爱华张伯庆
Owner 西安蓝绿卓生物科技有限公司
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