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Method for measuring nicotine content in gum base type chewing tobacco

A determination method, gum-based technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of inability to use gas chromatography, affect the accuracy of nicotine, unfavorable sampling, etc., achieve high yield and avoid being tested The effect of material loss and convenient sampling and detection

Active Publication Date: 2015-08-05
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in this method, the gum base insolubles and nicotine are finally present in the water phase of the lower layer, and the lower layer is turbid due to the existence of insolubles, which is not only unfavorable for sampling but also affects the accuracy of nicotine content determination
And because of the difficulty of sample injection due to the existence of insoluble matter, this analysis method cannot use the simpler and faster gas chromatography, but can only use liquid chromatography

Method used

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  • Method for measuring nicotine content in gum base type chewing tobacco
  • Method for measuring nicotine content in gum base type chewing tobacco
  • Method for measuring nicotine content in gum base type chewing tobacco

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for assaying nicotine content in gum-based chewing tobacco, comprising the following steps:

[0034] (1) Accurately weigh five gum-based chewing tobacco samples with a mass of (1.42±0.01)g (theoretical nicotine content is 0.247g / capsule), put them into a 100mL corked Erlenmeyer flask, add 8mL4mol / L sodium hydroxide aqueous solution and 40mL n-hexane, magnetic stirring extraction at room temperature for 110min, the magnetic stirring speed is 500r / min, to obtain a crude extract containing nicotine.

[0035] (2) After the extraction, the nicotine-containing crude extract was left to stand for 20 minutes, and the nicotine-containing supernatant was collected and dehydrated with 3.0 g of anhydrous sodium sulfate.

[0036] (3) Filtrating the obtained dehydrated organic phase layer containing nicotine with an organic phase needle filter to obtain a nicotine-containing extract.

[0037] (4) The nicotine-containing extract is detected by gas chromatography-mass spectr...

Embodiment 2

[0041] Embodiment 2—the sensitivity of nicotine content measuring instrument

[0042] Draw the standard curve:

[0043] Prepare nicotine n-hexane solutions with nicotine concentrations of 4 μg / ml, 10 μg / ml, 40 μg / ml, 100 μg / ml and 200 μg / ml respectively, and use quinoline n-hexane solutions with a concentration of 20 μg / ml as internal standard solutions , for gas chromatography analysis. The peak area ratio of nicotine and quinoline in the standard solution is plotted against the concentration ratio, the abscissa is the nicotine concentration, and the ordinate is the ratio of the instrument response value of nicotine and the internal standard. A standard curve was obtained, the regression equation of which was Y=0.727519X-2.86259, and the correlation coefficient was 0.9982. It can be seen that, within the range of nicotine concentration of 4-200mg / mL, the linearity of the equation is good, which is suitable for the quantitative analysis of nicotine. Wherein, the concentrati...

Embodiment 3

[0053] Embodiment 3—the mensuration of standard addition recovery

[0054] Take 3 portions of five gum-based chewing tobaccos with a nicotine content of 0.247g / capsule, put them into 100mL corked Erlenmeyer flasks, and add 8mL of 4mol / L to each Erlenmeyer flask in turn. NaOH aqueous solution and 40ml n-hexane solvent (which contains the internal standard quinoline whose concentration is 20mg / L). Use a magnetic stirrer to stir, and after the gum base dissolves (about 25 minutes), add 0.05 g, 0.10 g, and 0.15 g of standard tobacco powder with a nicotine content of 15.3 mg / g into each triangular flask (the amount of nicotine added is respectively 0.765mg, 1.530mg, 2.295mg), and continued to stir for 110min to obtain 5 bottles of crude extracts containing nicotine respectively. After standing still for 20 minutes, the respective supernatants were taken, dehydrated with anhydrous sodium sulfate, and treated with an organic phase membrane, and then analyzed and determined the nicot...

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Abstract

The invention relates to a method for measuring nicotine content in gum base type chewing tobacco. The method comprises the following steps: (1) extracting a gum base type chewing tobacco sample by using alkali liquor and a mixture which cannot be dissolved with the alkali liquor, thereby obtaining coarse extracting solution containing nicotine; (2) standing and layering the coarse extracting solution containing nicotine, and collecting an organic phase layer containing nicotine; (3) filtering the obtained organic phase layer containing nicotine by using an organic phase filter, thus obtaining nicotine-containing extract liquor; and (4) detecting the nicotine-containing extracting solution by using a gas chromatograph or a gas chromatograph-mass spectrometer, and obtaining the nicotine content in the gum base type chewing tobacco by adopting an internal standard method. According to the measuring method disclosed by the invention, the nicotine content is accurately measured, and the method is simple, convenient to operate and has a high popularization value.

Description

technical field [0001] The invention belongs to the technical field of measuring components of smokeless tobacco products, in particular to a method for measuring nicotine content in gum-based chewing tobacco. Background technique [0002] The promulgation of the ban on the spread of tobacco smoke in public places in the Framework Convention on Tobacco Control (FCTC) has had a strong impact on the development of traditional tobacco products such as cigarettes and cigars. The development of smokeless tobacco, a new type of tobacco, has become one of the consensus in the international tobacco field. Gum-based chewing tobacco is a new type of tobacco product that uses tobacco or tobacco extract as the effective component and edible gum base as the carrier to deliver nicotine to the human body through chewing. The outer layer can be coated with sugar. When consumers use this type of product, the nicotine and other ingredients embedded in the gum base are dissolved in saliva aft...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 袁大林汤建国孙志勇廖晓祥郑绪东雷萍尚善斋李寿波韩敬美陈永宽李正风陈建华朱瑞芝蔡炳彪李航谭正兰
Owner CHINA TOBACCO YUNNAN IND
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