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Methylobacterium capable of degrading chlorinated hydrocarbons and its application

A Methylobacillus, chlorinated hydrocarbon technology, applied in the field of environmental microorganisms, can solve problems such as limiting the application of chlorinated hydrocarbons

Inactive Publication Date: 2018-03-30
CHONGQING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The methane monooxygenase contained in methanotrophs can degrade chlorinated hydrocarbons through epoxidation, but because methanotrophs must achieve the degradation of chlorinated hydrocarbons by adding carbon sources in the form of co-metabolism, this is to a certain extent Limiting the application of aerobic oxidation of chlorinated hydrocarbons in engineering

Method used

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  • Methylobacterium capable of degrading chlorinated hydrocarbons and its application
  • Methylobacterium capable of degrading chlorinated hydrocarbons and its application
  • Methylobacterium capable of degrading chlorinated hydrocarbons and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Purification and Identification of Methylobacterium sp.R1

[0038] The bacterium is a Gram-negative bacterium with a colony diameter of 1-2 mm, translucent, regular round colonies, low protrusions, neat edges, uniform texture, glossy surface, and mostly yellow in color; it can be seen from the scanning electron microscope photos (see figure 1 ), Methylobacterium sp.R1 is short rod-shaped, with a diameter of 0.6-1um, a length of 2-4um, no spores, no flagella; obligate aerobic, the best growth temperature is 28-30 ℃, pH 7.0.

[0039]The sequencing of the DNA of the strain was entrusted to Dalian Bao Biology Co., Ltd., and the 16S rDNA base length of Methylobacterium sp.R1 was determined to be 1233bp (SEQ ID NO: 1). The base sequence was compared with the GenBank nucleic acid sequence database and found to be similar to methyl Methylobacterium sp.SAP462, Methylobacterium sp.XJLW and Methylobacterium radiotolerans have the highest homology, reaching more than 97%....

Embodiment 2

[0041] Example 2 Growth characteristics of Methylobacterium sp.R1

[0042] Configure 100ml of culture medium with a glucose concentration of 2g / L, put it into a 500ml serum bottle, and add Methylobacterium bacteria solution (OD 600nm 0.8) 3ml, cover the rubber stopper and shake well, put it into a shaker set at 30°C and 160 rpm for cultivation, and measure the OD of the bacterial solution regularly 600nm The value determines the growth of the bacteria. For the growth curve of Methylobacterium sp.R1 strain, see image 3 . The lag period of the strain is about 25h. After entering the logarithmic phase, the bacteria grew rapidly, and it took only 15 hours for the OD value to rise to 1.2, and then reached a plateau. The growth curve showed that the bacteria could obtain a larger concentration of bacteria with glucose as the medium.

Embodiment 3

[0043] Example 3 Degradation Effect of Methylobacterium sp.R1 on Different Chlorinated Hydrocarbons

[0044] Prepare solutions of trichlorethylene, dichlorethylene, vinyl chloride, tetrachloromethane, chloroform and dichloroethane with a concentration of 2g / L respectively, and add 100 μl of each to 100ml serum bottles containing 20ml of inorganic salt medium In the medium, the above-mentioned inorganic salt medium was sterilized by high-temperature steam at 121°C, and after cooling, 1ml of Methylobacterium bacteria liquid (OD 600nm 0.8), cover the rubber stopper and shake well, put it into a shaker and set it at 30°C and 160 rpm to cultivate for 40h, then use gas chromatography to measure the content of chlorinated hydrocarbons, and determine its degradation effect. OD 600nm The value determines the growth of the bacteria. It can be seen from Table 2 that Methylobacterium sp.R1 can degrade trichloroethylene, dichloroethylene, vinyl chloride, tetrachloromethane, trichlorometh...

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Abstract

The invention relates to a methylobacterium capable of degrading chlorinated hydrocarbons and an application thereof. The preservation number of the strain is CCTCC NO: M 2015046. The bacterium not only has high tolerance to chlorinated hydrocarbons, but also can grow with chlorinated hydrocarbons as the sole carbon source and energy source, overcomes the deficiency of the co-metabolic degradation process, and can maintain high activity in a poor environment, suitable for Applications in wastewater, drinking water, and soil remediation are expected to make new breakthroughs in the field of engineering applications for the biodegradation of chlorinated hydrocarbons.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and in particular relates to a methylobacterium capable of degrading chlorinated hydrocarbons and an application thereof. Background technique [0002] As important organic solvents and product intermediates, chlorinated hydrocarbons are widely used in chemical, pharmaceutical, pesticide and other fields. Due to improper use and storage, chlorinated hydrocarbons are discharged into the natural environment through volatilization, leakage, and wastewater discharge. A large number of studies in recent years have shown that chlorinated hydrocarbon pollutants such as trichlorethylene have potential "three-cause" (carcinogenic, teratogenic, mutagenic) effects and genotoxic effects. And central nervous system damage, causing a series of symptoms of body dysfunction, seriously endangering human health. With international and domestic demands on the ecological environment becoming h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C02F3/34B09C1/10C12R1/01A62D101/22C02F101/36
CPCA62D3/02B09C1/10C02F3/34C02F2101/36C12N1/205C12R2001/01
Inventor 张丽杰何芝赵天涛全学军邢志林
Owner CHONGQING UNIV OF TECH
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