DC cell based on BA46 antigen and targeting immune cell population, and preparation method and application thereof
A cell and antigen technology, applied to other methods of inserting foreign genetic material, cells modified by introducing foreign genetic material, animal cells, etc. High efficiency and good effect
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Embodiment 1
[0065] 1. Materials and equipment
[0066] 1. Laboratory level: GLP
[0067] 2. Instruments and equipment:
[0068] Negative 80-degree ultra-low temperature refrigerator (Zhongke Meiling, DW-HL388); ordinary refrigerator (Haier, double door, -20°C and 4°C); mold incubator (Shanghai Boxun, MJX-160B-Z); constant temperature water bath ( SSW-420-2S); CO 2 Incubator (HF240); low-speed centrifuge (Shanghai Anting, TDL-40C); inverted biological microscope (Kexin, model: BLD-1); biological safety cabinet (Suzhou purification, double); Rand) 1-10ml; 1 set of micropipettes (Dalong), 4 pieces; programmed cooling box (Thermo); liquid nitrogen tank (Jinfeng, YDS-120-216); PCR instrument; gel electrophoresis imaging system ; Electrophoresis apparatus; Thermostat;
[0069] 3. Cell culture consumables:
[0070] Cell culture bag (Takara company); 75cm 2 Culture bottle, 175cm 2 Culture bottle, 1.8ml cryopreservation tube, 1.5ml EP tube, 50ml centrifuge tube, and 15ml centrifuge tube are...
Embodiment 2
[0132] Embodiment 2: clinical treatment
[0133] The CTL cells prepared in Example 1 were reinfused into the patient, and the reinfusion effect was observed, as follows:
[0134] 20 BA46-positive breast cancer patients were divided into 2 groups (10 in group A and 10 in group B), wherein, for group A, sufficient CTL cells were obtained on day 14 and day 15 according to the method in Example 1 After that, reinfuse the patient, and the amount of cells reinfused each time is 1~2*10 9 ; Group B did not reinfuse cells (as a control). One blood collection and two consecutive reinfusions constituted a course of treatment. After 3 courses of treatment, observation was carried out.
[0135] The results showed that there was a significant difference between group A and group B. After reinfusion, all patients in group A felt less tired, had increased appetite, lessened pain, and gained weight. Among them, 6 patients had decreased tumor indicators; while The symptoms of group B were n...
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