Bacterial strain and method for producing welan gum
A technology of welan gum and strains is applied in the field of industrial microorganisms, which can solve the problems of different production strains and fermentation processes, and achieve the effects of inhibiting degradation, simple process and efficient production.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0030] Embodiment 1 Sphingomonas provided by the present invention Sphingomonas Morphological and physiological and biochemical identification of sp.WG strain
[0031] Sphingomonas WG provided by the invention ( Sphingomonas sp.WG) was isolated from the sea mud of Jiaozhou Bay, Qingdao, and the pure culture was obtained after serial dilution and plate plating. The strain morphology and physiological and biochemical reactions were identified by Gram staining, morphological observation, and Bojian Gram-negative bacteria identification system.
[0032] Sphingomonas WG ( Sphingomonas sp.WG) cultured on LB plates at 30°C for 24 hours to grow a single colony, the single colony was yellow, round, convex in the center, viscous, opaque, with neat edges and smooth surface (see attached figure 1 ); the strain is Gram-staining negative, the thallus is short rod-shaped, has flagella, and has no spores; its growth temperature is 20-40°C, growth pH is 4-9, and NaCl tolerance is 3%; oxid...
Embodiment 2
[0033] Example 2 Sphingomonas WG provided by the present invention ( Sphingomonas sp.WG) cloning and sequencing of the 16S rRNA gene
[0034] Sphingomonas WG provided by the invention ( Sphingomonassp.WG) 16S rRNA gene sequence characteristics: the WG strain was inoculated in LB medium, cultured at 30°C and 180 rpm for 16-18 h, and its genomic DNA was extracted using the Bacterial Genomic DNA Extraction Kit (Tiangen Biochemical Technology Co., Ltd.). The 16S rRNA gene fragment was obtained by PCR amplification using the upstream primer 5'-AGAGTTTGATCMTGGCTCAG-3' and the downstream primer 5'-TACGGYTACCTTGTTACGACTT-3'. The PCR conditions were as follows: 94°C for 5 min; 30 cycles of 94°C for 50 s, 56°C for 45 s, and 72°C for 90 s; 72°C for 10 min. After the 16S rRNA gene fragment obtained by PCR amplification was purified, it was connected to the pMD18-T vector with the quick connection kit, and the connection product was transformed into E. coli DH5α was screened on LB p...
Embodiment 3
[0035] Example 3 Preparation of Seed Solution
[0036] Solid medium: yeast extract 10 g / L, peptone 20 g / L, glucose 20 g / L, agar 20 g / L, pH7.5.
[0037] Liquid medium: yeast extract 10 g / L, peptone 20 g / L, glucose 20 g / L, pH 7.5.
[0038] The strain of Sphingomonas WG was streaked on YPD solid medium and cultured at a constant temperature of 30°C for 4 days to be activated. When yellow colonies grew, it was stored at 2-4°C for a short period of time until use. Put the activated single colony into a 250ml Erlenmeyer flask filled with YPD liquid medium, the volume of the Erlenmeyer flask was 50ml, and culture it at 30°C with a shaker speed of 230rpm for 18 hours to obtain the first-grade seed liquid. The primary seed liquid was inoculated into a 500 ml Erlenmeyer flask containing 100 ml liquid YPD medium at a 1.5% inoculation amount, and incubated at 30°C for 15 h at a shaker speed of 230 rpm to obtain a secondary seed liquid.
PUM
| Property | Measurement | Unit |
|---|---|---|
| temperature | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More