A device and application for simultaneous detection of plant photosynthesis and root oxygen secretion capacity

A technology for photosynthesis and root oxygen secretion, which is applied in measuring devices, color/spectral characteristic measurement, material analysis through optical means, etc. Can not freely adjust the light intensity and other problems, to achieve the effect of light weight, simple structure, easy to move

Active Publication Date: 2017-11-28
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, this type of anaerobic box is not equipped with a light layer, and the light intensity cannot be adjusted freely, and the light is very important for the photosynthesis of plants, which directly affects the oxygen secretion of the roots of the plants; The environment of inert gas itself will have an impact on the growth of plants, therefore, it cannot truly reflect the oxygen secreting capacity of plant roots
In addition, the above-mentioned anaerobic box is bulky, inconvenient to carry, and costly, and requires multiple vacuum pumps for the pumping process, which is not suitable for use in small laboratories

Method used

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  • A device and application for simultaneous detection of plant photosynthesis and root oxygen secretion capacity
  • A device and application for simultaneous detection of plant photosynthesis and root oxygen secretion capacity
  • A device and application for simultaneous detection of plant photosynthesis and root oxygen secretion capacity

Examples

Experimental program
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Embodiment 1

[0042] Such as Figure 1~2 As shown, this embodiment provides a device for simultaneously detecting plant photosynthesis and root oxygen secretion capacity, including a lighting system unit A, a multi-channel plant photosynthesis-oxygen secretion unit B and an oxygen secretion detection unit arranged from top to bottom c.

[0043] The lighting system unit A includes 6 light sources 1, that is, 6 fluorescent lamp tubes, each of which is connected in parallel and controlled by an independent switch 9, which is beneficial to flexibly adjust the light intensity.

[0044] The multi-channel plant photosynthetic-oxygen-secreting unit B includes a multi-channel plate 20 , and several glass tubes 6 , plugs 3 and sealing rings 5 ​​used in conjunction with the multi-channel plate 20 . Wherein, the stopper 3 is a rubber stopper; the multi-channel plate 20 is provided with several plate holes 8 (the number of the plate holes 8 is the same as that of the glass tubes 6), and the hole diamet...

Embodiment 2

[0048] A method for in-situ detection of the oxygen-secreting capacity of roots of different plants, comprising the following steps:

[0049] S1. Construction of the device described in Example 1.

[0050] S2. Slide the front side wall of the inert gas glove operation box, open the box body, put reagents, pipette guns and other supplies necessary for the test operation into the box body, slide the front side wall to close the box body, and plug it with a rubber stopper Tighten the plate hole of the multi-channel plate, and seal the entire box with a sealing strip.

[0051] S3. Connect the nitrogen inert gas supply bottle, flow meter, pressure reducing valve and other components in sequence, open the pressure reducing valve on the inert gas supply bottle, open the air inlet and exhaust valves of the box, and adjust the flow meter to make the nitrogen The flow rate is 5L / min, and the air intake pipe in the box is moved to every corner of the box through the gloves connected to ...

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Abstract

The invention discloses a device for simultaneously performing plant photosynthesis and root system oxygen secretion capacity detection and its application. The device comprises a top-to-bottom illumination system unit, a multi-channel plant photosynthesis-oxygen secretion unit and an oxygen secretion detection unit; the illumination system unit is fixed on the multi-channel plant photosynthesis-oxygen secretion unit by a support; the multi-channel plant The photosynthetic-oxygen secreting unit includes a multi-channel plate, and several glass tubes, plugs and sealing rings used in conjunction with the multi-channel plate; several plate holes are arranged on the multi-channel plate; the edge of the glass tube protrudes outward, and the glass tube passes through The sealing ring is fixed on the plate hole; the glass tube is provided with a sampling port, and the sampling tube is sealed and connected with the glass tube through the sampling port; one end of the sampling tube is inserted into the bottom of the glass tube, and the other end is connected with a sampling device outside the glass tube; The oxygen leakage detection unit is an anaerobic operation unit with a multi-channel plate as the top. The device of the invention can perform in-situ detection of the oxygen secreting ability of the root system while the plant is photosynthesizing.

Description

technical field [0001] The invention relates to a device and method for detecting the oxygen secretion capacity of plant roots, in particular to a device and application for simultaneously detecting plant photosynthesis and root system oxygen secretion capacity. Background technique [0002] Wetland plants are an important part of constructed wetlands. They can purify water by directly absorbing and utilizing available nutrients in sewage, absorbing and enriching heavy metals and some toxic and harmful substances. Wetland plant roots have developed oxygen-secreting ability, and can form aerobic, micro-aerobic and anaerobic zones in the root zone, providing various suitable microenvironments for microorganisms, thereby enhancing the purification ability of constructed wetlands for sewage. Therefore, the oxygen secreting ability of wetland plants' roots is an important index to evaluate their sewage purification ability. [0003] At present, the methods for measuring the oxyg...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/00G01N21/31
Inventor 杨扬林剑华邰义萍乔永民王团团
Owner JINAN UNIVERSITY
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