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Hybrid seed production method for Erigeron breviscapus (Vant.) Hand.-Mazz.

A technology for hybrid seed production and Dengzhanhua, applied in the directions of botanical equipment and methods, applications, and plant genetic improvement, can solve the problems of self-incompatibility, no public report, lack of human, material and financial resources, etc. The effect of high purity of seed hybrids, neat progeny characters and simple and easy method

Inactive Publication Date: 2015-09-09
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As far as the breeding of Erigeron breviscapus is concerned, so far no new varieties with superior breeding have come out. The main reason is that (1) the breeding of Erigeron breviscapus cultivars was carried out late, and the human, material and financial resources for this research are slightly insufficient; (2) ) The breeding goals of inbred lines are not very clear and clear; (3) The identification technology of germplasm resources is still slightly insufficient; (4) The cross-pollination technology cannot solve the problem.
The flowers of Erigeron breviscapus are inflorescences, which are composed of several small flowers. It is extremely difficult to artificially detassell. One small flower only bears one seed for pollination, and it is difficult to meet the demand for seeds in production.
In addition, Erigeron breviscapus has strong self-incompatibility, and the average self-seed setting rate is less than 10%.
[0005] Breviscapita hybrid seed production method of the present invention, through literature search, no public report identical with the present invention is seen

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] a. Parental selection: the female parent y-4-2-5 was selected from wild domesticated materials in Yuxi, with a self-compatibility rate of 1.25%; the male parent q-14 was selected from a single plant of Qianshan No. 2, with a self-compatibility rate of 4.33 %;

[0024] b. Planting options: Plastic greenhouses are isolated from the outside world with 80-mesh insect-proof nets. The door is a sliding door with an insect-proof net curtain inside the door. No scutellaria has been planted in the greenhouse before;

[0025] c. Soil preparation and furrowing: apply 3000kg of decomposed manure per mu, 15kg of compound fertilizer (N-P-K=15-15-15), deeply plow the soil to 20-25 cm, and make a furrow with a width of 120 cm and a height of 10 cm;

[0026] d. Sowing seedlings: using PE plug substrate for seedling cultivation, the first batch of male parents was sown on January 6, the second batch was sown on January 27; the female parent was sown on January 13;

[0027] e. Transplant...

Embodiment 2

[0034] a. Parental selection: the female parent q-1 was selected from a single plant of Qianshan No. 2, with a self-compatibility rate of 0.33%; the male parent y-4-1-2 was selected from wild domesticated materials in Yuxi, with a self-compatibility rate of 0.67 %;

[0035] b. Farming options: Plastic greenhouses are isolated from the outside world with 80-mesh insect-proof nets. The door is a sliding door with an insect-proof net curtain inside the door. No scutellaria has been planted in the greenhouse before.

[0036] c. Soil preparation and furrowing: apply 2500kg of decomposed manure per mu, 20kg of compound fertilizer (N-P-K=15-15-15), deeply plow the soil to 20-25 cm, and make a furrow with a width of 90 cm and a height of 20 cm;

[0037] d. Sowing seedlings: using foam trays to float seedlings, the first batch of male parents was sown on September 3, the second batch was sown on September 24; the female parents were sown on September 10;

[0038] e. Transplanting and ...

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Abstract

The invention relates to a hybrid seed production method for Erigeron breviscapus (Vant.) Hand.-Mazz., and belongs to the technical field of Chinese herbal medicine reproduction. According to the method, an inbred line with the self-compatibility rate lower than 2% is selected as a female parent, an isolatable protected area is taken as a seed farm, soil preparation is performed to form a bed, a male parent is subjected to sowing by stages for seedling raising one week earlier than the female parent and two weeks later than the female parent respectively, colonization is performed in the line ratio of male and female parents being 1:2 at the line interval of 7 cm when seedlings grow to have three leaves and one tip, 0.05% KH2PO4 fertilizer is sprayed for concentrated flower-formation promotion, supplementary pollination for wedge-shaped Erigeron breviscapus (Vant.) Hand.-Mazz. blades is performed, wilted male parent flowers are removed timely in the pollination period, and timely anti-falling harvest is performed. The method comprises the steps of parental selection, seed farm selection, soil preparation for bed forming, soilless seedling raising, seedling transplanting for colonization, concentrated flower-formation promotion, supplementary pollination, pollination period management and seed harvest. The method has the benefits of simplicity, easiness, high seed production quantity, higher hybrid purity, more orderly offspring characteristics and capability of realizing heterosis breeding of the Erigeron breviscapus (Vant.) Hand.-Mazz..

Description

technical field [0001] The invention relates to a hybrid seed production method of scutellaria breviscapus, which belongs to the technical field of Chinese herbal medicine propagation. Background technique [0002] Erigeron ( Erigeron breviscapus (Vant.) Hand.-Mazz. ), also known as Erigeron breviscapus Asarum, breviscapella, is a perennial plant of the family Asteraceae. Clinically, it is mainly used for wind-cold-damp arthralgia, stroke paralysis, toothache, cold and so on. The results of medicinal chemical research show that the main medicinal active ingredients of breviscapine are breviscapine, pyromeiconic acid and caffeoyl compounds. At present, there are more than 80 manufacturers of breviscapine preparations in China. The main products are extracts, capsules, injections (water injections and freeze-dried powder injections), tablets, granules and oral liquids, with nearly 20 varieties in six categories. [0003] With the expansion of market demand for Erigeron bre...

Claims

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Application Information

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IPC IPC(8): A01H1/02
Inventor 岳艳玲杨生超朱海山盛鹏张广辉郭瑛琪崔海芳
Owner YUNNAN AGRICULTURAL UNIVERSITY
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