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Pediococcus acidilactici P3-4, screening and identifying method thereof and application of pediococcus acidilactici P3-4 in degradation of erythromycin and roxithromycin

A technology of Pediococcus lactis and roxithromycin, applied in the field of screening, identification and application, microbiology, can solve the problems of human intestinal bacterial flora imbalance, change the micro-ecological environment of human intestinal flora, and reduce secondary pollution , low cost, cost reduction effect

Active Publication Date: 2015-09-16
潍坊中创生物科技有限公司
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Problems solved by technology

Long-term use will change the micro-ecological environment of the human intestinal flora, causing the imbalance of the human intestinal bacterial flora, and at the same time prone to bacterial resistance

Method used

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  • Pediococcus acidilactici P3-4, screening and identifying method thereof and application of pediococcus acidilactici P3-4 in degradation of erythromycin and roxithromycin
  • Pediococcus acidilactici P3-4, screening and identifying method thereof and application of pediococcus acidilactici P3-4 in degradation of erythromycin and roxithromycin
  • Pediococcus acidilactici P3-4, screening and identifying method thereof and application of pediococcus acidilactici P3-4 in degradation of erythromycin and roxithromycin

Examples

Experimental program
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Embodiment

[0054] Example, a strain of Pediococcus lactis ( Pediococcus acidilactici ) P3-4, preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms, and its preservation number is: CGMCC No.10574.

[0055] Pediococcus lactis above ( Pediococcus acidilactici ) The screening method of P3-4, comprising the following steps:

[0056] 1 Experimental materials

[0057] 1.1 Experimental strains

[0058] Sample source

[0059] Collect soil samples from different locations in Weifang, Yantai and other regions

[0060] 1.2 Experimental Instruments

[0061] Vertical pressure steam sterilizer (Hirayama HVE-50, Japan); biological safety cabinet (XUN BSC-1300ⅡA / B3); microscope (OLYMPUS CX21); high performance liquid chromatography (Aglient 1260); high performance liquid chromatography / mass spectrometry / Mass spectrometer (Aglient 1260-6430); analytical balance (Mettler Toledo PL 602-S, Switzerland); constant temperature incubator (MMM Incucell 111, Ge...

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Abstract

The invention discloses pediococcus acidilactici P3-4 which is preserved in a common microorganism center of the Chinese microorganism culture preservation management council. The preservation serial number of the pediococcus acidilactici P3-4 is CGMCC No.10574. A novel method for screening lactic acid bacteria is established based on the combination of the microorganism method and the chemical method, a culture medium is selected through antibiotics for primary screening and secondary screening, and a lactic acid bacterium which can degrade erythromycin and roxithromycin is screened out from soil. A reasonable and effective method for screening a strain for degrading erythromycin and roxithromycin is established, the proper required lactic acid bacterium strain is obtained, primary screening and secondary screening of the lactic acid bacterium for degrading erythromycin and roxithromycin are achieved through separation, purification and screening of lactic acid bacteria, and the safe and effective strain for degrading erythromycin and roxithromycin is obtained through screening. The pediococcus acidilactici P3-4 is used for treatment of pollution of erythromycin and roxithromycin and has the advantages that secondary pollution is avoided, treatment efficiency is high, the application range is wide, and cost is low. More options are provided for treating antibiotic pollution in complicated environments through microorganisms.

Description

technical field [0001] The invention relates to a strain of microorganism, screening identification and application, in particular to a strain of Pediococcus lactis P3-4 and its screening identification and application in degrading erythromycin and roxithromycin, belonging to the technical field of detection and application of microorganisms. Background technique [0002] Lactic acid bacteria are mainly distributed in a slightly acidic environment rich in mineral and organic nutrients. Dairy products, meat products, kimchi, fruit wine, vegetables and other materials are rich in lactic acid bacteria. In addition, animal digestive tracts are also important sources of lactic acid bacteria. Lactic acid bacteria play an important role in the nutrition and health of animals. They have the effects of antibacterial and anti-inflammatory, anti-oxidation, anti-tumor, lowering cholesterol, and improving immunity. [0003] Macrolide antibiotics have outstanding antibacterial activity ag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A62D3/02C12N1/02C12R1/01
CPCA62D3/02C12N1/02C12N1/205C12R2001/01
Inventor 孙军郝莹赵晗许文娟马荣桧
Owner 潍坊中创生物科技有限公司
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