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A strain of Halomonas with denitrification ability and its application

A Halomonas, biological denitrification technology, applied in the direction of bacteria, microorganism-based methods, water/sludge/sewage treatment, etc., can solve the problem of few good strains and achieve strong tolerance and high denitrification efficiency High and carbon-saving effect

Active Publication Date: 2018-05-15
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although there are many studies on the isolation, screening and mechanism of aerobic denitrifying bacteria at home and abroad, there are few good strains, which still cannot meet the needs of current industrial production.

Method used

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  • A strain of Halomonas with denitrification ability and its application
  • A strain of Halomonas with denitrification ability and its application
  • A strain of Halomonas with denitrification ability and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Enrichment, isolation and identification of Example 1 Halomonas sp. (Halomonas sp.) CYQ1-6-1

[0043] 1 Enrichment and separation of bacterial strains: Take 10 g of the sludge sample from the primary sedimentation tank of Xiamen Qianpu Sewage Treatment Plant, add 90 ml of enrichment medium (sodium acetate 1 g / L, sodium citrate 2.5 g / L, NaCl 20 g / L, KH 2 PO 4 2g / L, Na 2 HPO 4 3g / L, MgSO 4 ·7H 2 O0.05g / L, FeSO 4 ·7H 2 O 0.02g / L, CaCl 2 0.02g / L, distilled water 1000mL, pH 7.6~8.2, sterilized at 121℃ for 20min), with 0.5g / L NO 2 - -N as the only nitrogen source, 28 ℃, 160rpm / min shaking culture, the first enrichment of aerobic denitrifying bacteria. After 15 days, take 10mL sample liquid from the initial enrichment culture medium, transfer it to fresh enrichment culture medium, and add 1.0g / L NO 2 - -N as the only nitrogen source, 28°C, 160rpm / min shaking culture, for the second enrichment. After 15 days, take 10mL sample solution from the secondary enrichment m...

Embodiment 2

[0050] Example 2 Optimization of Denitrification Conditions for Halomonas sp. CYQ1-6-1 in Simulated Wastewater

[0051] Scrape fresh bacteria from the MA plate, resuspend with sterile sea water, and adjust the bacterial concentration to 10 9 cfu / mL, according to the inoculation amount of 0.2% to inoculate to contain a certain concentration of NO 3 - -N or NO 2 - In the simulated wastewater of -N, the denitrification conditions of the strains were measured at different carbon sources, pH values, temperatures, salinities, and C / N ratios, and the denitrification conditions were optimized.

[0052] Simulated wastewater: KH 2 PO 4 2g / L, Na 2 HPO 4 3g / L, MgSO 4 ·7H 2 O 0.05g / L, FeSO 4 ·7H 2 O 0.02g / L, CaCl 2 0.02g / L, distilled water 1000mL, sterilized at 121℃ for 20min.

[0053] Experimental determination method: the determination of nitrate nitrogen is determined by the zinc cadmium reduction method of GB T12763;

[0054] 1. Effect of different carbon sources on the d...

Embodiment 3

[0059] Example 3 Denitrification effect of Halomonas sp. CYQ1-6-1 in simulated wastewater under optimal conditions

[0060] Pick a single colony of Halomonas sp. CYQ1-6-1 and streak it onto a Marine Agar solid plate, culture it at 28°C for 24 hours, take it out, scrape the fresh bacteria, and use nitrogen-free medium to make a bacterial suspension solution, the strain concentration was adjusted to 10 9 cfu / mL, according to the inoculation amount of 0.2% to inoculate with 100mg / L NO 3 - -N or NO 2 - -N is the only nitrogen source in simulated wastewater (sodium succinate 3.37g / L, NaCl 20g / L, KH 2 PO 4 2g / L, Na 2 HPO 4 3g / L, MgSO 4 ·7H 2 O 0.05g / L, FeSO 4 ·7H 2 O 0.02g / L, CaCl 2 0.02g / L, distilled water 1000mL, pH 7.6, sterilized at 121°C for 20min), cultivated in a shaker at 30°C, 160r / min, and sampled every 4h to detect the concentration of nitrite and nitrate in the wastewater, and the concentration of bacteria OD 600 . see results Figure 6-7 , the results sho...

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Abstract

A salt monocylla and its application with nitrogen dehydration capacity belongs to the field of microbial application technology.Cyq1‑6‑1, Halomonas SP. Cyq1‑6‑1, the preservation number CCTCC NO: M 2015266.Salomonas (Halomonas SP.) CYQ1‑6‑1 can quickly and efficiently remove nitrate and nitrite in the water body, and have strong tolerance and removal capacity for high concentrations of high concentration.The salt monocylla is (Halomonas SP.) CYQ1‑6‑1 was used in the treatment of high concentration of nitrite wastewater, nitrogen -containing wastewater, nitrogen -off, and aquaculture nitrite removal.The nitrogen -containing wastewater includes but is not limited to nitrate wastewater, nitrite wastewater, ammonia nitrogen waste water, etc.The salt monocystine is (Halomonas SP.) CYQ1‑6‑1 can tolerate nitrite to 4000mg / L.

Description

technical field [0001] The invention belongs to the technical field of microorganism application, and in particular relates to a strain of Halomonas with denitrification ability and its application. Background technique [0002] In natural water bodies, nitrogen exists in nine different valence forms from -3 to +5. Under the joint action of biotic and abiotic factors, they are continuously transferred and transformed in the water body, forming a complex dynamic cycle. Among them, ammonia nitrogen and nitrite can lead to acidification and eutrophication of lakes, rivers, and offshore oceans. At the same time, as potentially toxic inorganic nitrogen compounds, they also have a toxic effect on aquaculture organisms. How to remove nitrogen pollution in water economically and efficiently has become a key research content in the field of water pollution control. [0003] At present, the methods used in denitrification of water body mainly include physical method, chemical method ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16
CPCC02F3/34C02F2101/16C12N1/205C12R2001/01
Inventor 邵宗泽王丽萍李小义
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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