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A Salt-tolerant Denitrification Bacteria Using Nitrite as Nitrogen Source and Its Application

A technology of denitrifying bacteria and nitrite, which is applied in the field of salt-tolerant denitrifying bacteria, and can solve problems such as the inability to remove nitrite nitrogen

Active Publication Date: 2021-04-23
NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention aims to solve the technical problem that the denitrification ability of the existing denitrifying bacteria is inhibited by salt and cannot remove nitrite nitrogen in saline wastewater, and provides a salt-tolerant denitrifying bacterium with nitrite as a nitrogen source and its application

Method used

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  • A Salt-tolerant Denitrification Bacteria Using Nitrite as Nitrogen Source and Its Application
  • A Salt-tolerant Denitrification Bacteria Using Nitrite as Nitrogen Source and Its Application
  • A Salt-tolerant Denitrification Bacteria Using Nitrite as Nitrogen Source and Its Application

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specific Embodiment approach 1

[0022] Embodiment 1: In this embodiment, a salt-tolerant denitrifying bacterium using nitrite as a nitrogen source as a habitat plant, Pannonibacter phragmitetus F1, is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, The deposit address is No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing. The deposit date is March 25, 2019, and the deposit number is CGMCC No.17432.

[0023] The colony of the habitat plant Pannonibacter phragmitetus F1 in this embodiment is dot-shaped, extremely small, with neat edges, smooth and shiny surface. Under the microscope, the bacteria are rod-shaped and are Gram-negative bacteria.

[0024] The physiological and biochemical characteristics of the present embodiment Pannonibacter phragmitetus F1, as shown in Table 1:

[0025] Table 1 Physiological and biochemical characteristics of Pannonibacter phragmitetus F1

[0026]

[0027] +: growth or positive; -: no growth ...

specific Embodiment approach 2

[0028] Specific embodiment two: the screening method of the habitat plant Pannonibacter phragmitetus F1 in this embodiment is realized according to the following steps:

[0029] Step 1: Take 2mL of seawall muddy water sample collected from Zhuanghe City, Dalian City, Liaoning Province (39°38′31″N and 122°58′19″E), put it into a 250mL Erlenmeyer flask, and add 200mL of strain denitrification medium ; Use a sealing film to seal the mouth of the bottle to create an anoxic environment; culture at a constant temperature for 5 to 7 days at a temperature of 30°C to obtain an enriched culture solution;

[0030] Step 2: Gradiently dilute the enriched culture solution in Step 1 using the doubling dilution method, pipette 0.5mL of each dilution suspension into the strain screening plate culture medium (which has been placed overnight, no bacteria growth), and coat with a glass triangle. Spread the enriched culture solution evenly with a cloth stick, and pour it into the second layer of s...

specific Embodiment approach 3

[0049] Specific embodiment three: In this embodiment, the salt-tolerant determination of the habitat plant Pannonibacter phragmitetus F1 is as follows:

[0050] Prepare screening plate media with different salinity gradients, that is, the NaCl concentration is 0g / L, 30g / L, 50g / L, 70g / L and 100g / L respectively; inoculate Pannonibacter phragmitetus F1 into the culture media of each salinity Put it on the plate and culture it in a constant temperature incubator at 30°C for 5 days. Whether the strain grows is used as the basis for whether the strain can withstand the corresponding salinity; the culture medium not inoculated with denitrifying bacteria is used as the blank control, and each group has 3 parallels.

[0051] Table 2 shows the growth of Pannonibacter phragmitetus F1 in different salinity gradient media. After 1 day of culture, there were no colonies at all salinity media; after 2 days of culture, there were obvious colonies on the media containing 0g / L and 30g / L NaCl, a...

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Abstract

A salt-tolerant denitrification bacterium using nitrite as a nitrogen source and application thereof, the invention relates to the field of environmental microorganisms, in particular to a salt-tolerant denitrification bacterium using nitrite as a nitrogen source. The invention aims to solve the technical problem that the denitrification ability of the existing denitrifying bacteria is inhibited by the salt, and the nitrite nitrogen in the saline wastewater cannot be removed. The strain is Pannonibacter phragmitetus F1, which is a plant-dwelling plant, and it is preserved in the General Microbiology Center of China Committee for the Collection of Microorganisms. The preservation date is March 25, 2019, and the preservation number is CGMCC No.17432. The strain can denitrify and denitrify under saline conditions. The bacterial strain of the invention is used for removing nitrite nitrogen pollutants in water bodies.

Description

technical field [0001] The invention relates to the field of environmental microorganisms, in particular to salt-tolerant denitrification bacteria using nitrite as a nitrogen source. Background technique [0002] With the improvement of human life quality, a large amount of high-nitrogen wastewater is produced, and high-nitrogen pollution has become a major problem that needs to be solved urgently. Among them, the nitrite nitrogen in the water body is highly toxic, which can easily cause the deterioration of the water body, affect the normal growth of aquatic animals and humans, and even endanger life. Nitrite nitrogen pollution comes from a wide range of sources, mainly from domestic sewage, industrial wastewater and aquaculture wastewater. Nitrogen pollutants in domestic sewage are converted into ammonia through microbial degradation and ammonification, and then converted into nitrite through the oxidation of nitrosating bacteria, resulting in nitrite nitrogen pollution. ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16
CPCC02F3/34C02F2101/166C12N1/205C12R2001/01
Inventor 祝惠王鑫壹阎百兴陈欣于翔霏
Owner NORTHEAST INST OF GEOGRAPHY & AGRIECOLOGY C A S
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