A Salt-tolerant Denitrification Bacteria Using Nitrite as Nitrogen Source and Its Application
A technology of denitrifying bacteria and nitrite, which is applied in the field of salt-tolerant denitrifying bacteria, and can solve problems such as the inability to remove nitrite nitrogen
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
specific Embodiment approach 1
[0022] Embodiment 1: In this embodiment, a salt-tolerant denitrifying bacterium using nitrite as a nitrogen source as a habitat plant, Pannonibacter phragmitetus F1, is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, The deposit address is No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing. The deposit date is March 25, 2019, and the deposit number is CGMCC No.17432.
[0023] The colony of the habitat plant Pannonibacter phragmitetus F1 in this embodiment is dot-shaped, extremely small, with neat edges, smooth and shiny surface. Under the microscope, the bacteria are rod-shaped and are Gram-negative bacteria.
[0024] The physiological and biochemical characteristics of the present embodiment Pannonibacter phragmitetus F1, as shown in Table 1:
[0025] Table 1 Physiological and biochemical characteristics of Pannonibacter phragmitetus F1
[0026]
[0027] +: growth or positive; -: no growth ...
specific Embodiment approach 2
[0028] Specific embodiment two: the screening method of the habitat plant Pannonibacter phragmitetus F1 in this embodiment is realized according to the following steps:
[0029] Step 1: Take 2mL of seawall muddy water sample collected from Zhuanghe City, Dalian City, Liaoning Province (39°38′31″N and 122°58′19″E), put it into a 250mL Erlenmeyer flask, and add 200mL of strain denitrification medium ; Use a sealing film to seal the mouth of the bottle to create an anoxic environment; culture at a constant temperature for 5 to 7 days at a temperature of 30°C to obtain an enriched culture solution;
[0030] Step 2: Gradiently dilute the enriched culture solution in Step 1 using the doubling dilution method, pipette 0.5mL of each dilution suspension into the strain screening plate culture medium (which has been placed overnight, no bacteria growth), and coat with a glass triangle. Spread the enriched culture solution evenly with a cloth stick, and pour it into the second layer of s...
specific Embodiment approach 3
[0049] Specific embodiment three: In this embodiment, the salt-tolerant determination of the habitat plant Pannonibacter phragmitetus F1 is as follows:
[0050] Prepare screening plate media with different salinity gradients, that is, the NaCl concentration is 0g / L, 30g / L, 50g / L, 70g / L and 100g / L respectively; inoculate Pannonibacter phragmitetus F1 into the culture media of each salinity Put it on the plate and culture it in a constant temperature incubator at 30°C for 5 days. Whether the strain grows is used as the basis for whether the strain can withstand the corresponding salinity; the culture medium not inoculated with denitrifying bacteria is used as the blank control, and each group has 3 parallels.
[0051] Table 2 shows the growth of Pannonibacter phragmitetus F1 in different salinity gradient media. After 1 day of culture, there were no colonies at all salinity media; after 2 days of culture, there were obvious colonies on the media containing 0g / L and 30g / L NaCl, a...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com