Method for increasing kitchen waste high solid-state fermentation produced biogas
A technology of food waste and solid-state fermentation, applied in fermentation, biosynthesis, waste fuel, etc., to achieve the effects of increasing economic benefits, easy control, and improving efficiency
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[0023] The experimental device adopts the automatic methane potential analysis system (AMPTS), the reaction temperature is 55±2°C, and the gas volume is counted by AMPTS v5.0 software. During the acid-resistant acclimation culture process, the methane content in the biogas, the GAD activity in the fermentation broth, the H + -ATP concentration was measured; in the high solid-state anaerobic fermentation process, methane content in biogas, amylase activity and protease activity in fermentation liquid were measured, and the determination methods were all analyzed by national standard methods (Table 1).
[0024] Table 1 Analysis items and methods
[0025]
[0026] The acid-resistant acclimation culture of methanogens is to add 200g of sludge, 20mL of nutrient solution (Table 2), 1.5g of glucose and quantitative sodium butyrate (Table 3) into a 500mL serum bottle, and add deionized water to 500mL, every 2 days Add 1.5 g of glucose. Five groups of experiments were set up, mark...
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