A kind of culture medium of purple coccus

A culture medium, Porphyra technology, applied in the direction of unicellular algae, microorganism-based methods, microorganisms, etc., can solve the problems of slow growth and low biomass yield of Porphyra, and achieve rapid proliferation and high cell density. , the effect of increasing the concentration of algal cells

Active Publication Date: 2018-02-09
NINGBO FUTIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the main problem of large-scale cultivation of Porphyridium is that the growth is relatively slow and the biomass yield is low, and a suitable medium can greatly promote the growth of Porphyridium

Method used

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  • A kind of culture medium of purple coccus
  • A kind of culture medium of purple coccus
  • A kind of culture medium of purple coccus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] (1) Use natural seawater to configure the Porphyridium culture medium of the following formula: Co(NH 2 ) 2 20mg / L, NaH 2 PO 4 8mg / L, ferric citrate 1mg / L, Na 2 EDTA 7.5mg / L, lutetium chloride 0.1mg / L, titanium citrate 0.1mg / L, VB 1 0.006mg / L, VB 12 0.05mg / L, ZnSO 4 4H 2 O 15μg / L, MnCl 2 4H 2 O 150μg / L, CuSO 4 ·5H 2 O 7.5 μg / L, Na 2 MoO 4 2H 2 O 6 μg / L, CoCl 2 ·6H 2 O 7.5 μg / L.

[0012] (2) Inoculate the Porphyridium algae in the logarithmic growth phase into the medium for cultivation, light for 10 hours every day, and the light intensity is about 50 μmol / (m 2 s), the temperature of the incubator was controlled at about 25 degrees Celsius, and the cell concentration of Porphyridum in the culture medium was recorded at regular intervals, see Table 1 for details.

Embodiment 2

[0014] (1) Use natural seawater to configure the Porphyridium culture medium of the following formula: Co(NH 2 ) 2 30mg / L, NaH 2 PO 4 12mg / L, ferric citrate 2mg / L, Na 2 EDTA 7.5mg / L, lutetium chloride 1mg / L, titanium citrate 1mg / L, VB 1 0.006mg / L, VB 12 0.05mg / L, ZnSO 4 4H 2 O 15μg / L, MnCl 2 4H 2 O 150μg / L, CuSO 4 ·5H 2 O 7.5 μg / L, Na 2 MoO 4 2H 2 O 6 μg / L, CoCl 2 ·6H 2 O 7.5 μg / L.

[0015] (2) Inoculate the Porphyridium algae in the logarithmic growth phase into the medium for cultivation, light for 10 hours every day, and the light intensity is about 50 μmol / (m 2 s), the temperature of the incubator was controlled at about 25 degrees Celsius, and the cell concentration of Porphyridum in the culture medium was recorded at regular intervals, see Table 1 for details.

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Abstract

The invention discloses a porphyridium culture medium. The porphyridium culture medium comprises the following components: by mass concentration, 20-30 mg / L of Co(NH2)2, 8-12 mg / L of NaH2PO4, 1-2 mg / L of ferric citrate, 5-10 mg / L of Na2EDTA, 0.005-0.007 mg / L of VB1, 0.04-0.06 mg / L of VB12, 10-20 micron g / L ofZnO4.4H2O, 100-200 micron g / L of MnCl2.4H2O, 5-10 micron g / L of CuSO4.5H2O, 5-7.5 micron g / L of Na2MoO4.2H20 and 5-10 micron g / L of CoCl2.6H2O, and further comprises 0.1-l mg / L of plant growth regulator citricacide-titatnium chelate and 0.1-l mg / L of lutetium salt. When porphyridium is cultured by using the culture medium, the porphyridium has the advantages that the growth velocity is high and the cell density is large. The porphyridium culture medium is suitable for large scale batch production.

Description

technical field [0001] The invention relates to an algae culture medium, in particular to a porphyridium culture medium. Background technique [0002] Porphyridium cruentum belongs to Rhodophyta, Protoerythrophyta, Porphyrides, Porphyridaceae, and Porphyridium genus. It is a marine single-celled microalgae that presents purple or deep red in different growth stages. It contains Rich in polyunsaturated fatty acid PUFAs such as arachidonic acid AA and eicosapentaenoic acid EPA. AA and EPA are important essential fatty acids in the human body. AA has the functions of regulating cardiac excitability, participating in neuroendocrine, promoting cell division and inhibiting platelet aggregation. The precursor of triene can improve blood lipid quality, reduce cholesterol, blood viscosity and blood sugar concentration, and can effectively treat and prevent coronary heart disease and other cardiovascular and cerebrovascular diseases, as well as anti-inflammatory and anti-allergic eff...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 王兆伟彭小伟杜学星王弢靳立兵王哲恩王闪
Owner NINGBO FUTIAN BIOTECH
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