Hantavirus-like particles comprising CD40l, preparation method and application thereof

Abstract

The invention relates to a hantavirus sample particle with CD40L and a preparation method and application of the hantavirus sample particle. For a VLP composed of a plurality of proteins, a rhabdovirus for expressing each protein needs to be prepared on the basis of an insect cell expression system, insect cells are infected according to different proportions, the common infection rate needs to be explored through a lot of work, and the rhabdoviruses mixed in a product cause a certain trouble to later purification. On the basis of a mammal cell expression system, by establishing eukaryotic expression vectors and cotransfection dhfr-CHO cells, the hantavirus sample particle with CD40L is assembled. All components of the hantavirus sample particle can be expressed, the established eukaryotic expression vectors contain various screening marks, a cell strain stably expressing the hantavirus sample particle can be established through screening, large-scale production and preparation are facilitated, and the particle can be used for preventing hemorrhagic fever with renal syndrome caused by hantavirus infection.

Description

technical field [0001] The invention relates to a vaccine, in particular to a hantavirus-like particle containing CD40L and a preparation method and application thereof. Background technique [0002] Hemorrhagic fever with renal syndrome (HFRS) is an acute viral infectious disease caused by Hantavirus. At present, there is no specific and effective treatment drug for this disease, and it mainly relies on vaccines for prevention. Although inactivated virus vaccines for hemorrhagic fever with renal syndrome have been developed in China, such vaccines stimulate the body to produce low neutralizing antibody titers and cannot effectively stimulate the body to produce cellular immune responses. In addition, primary cell and brain tissue inactivated vaccines Most of the raw materials come from animals, and the quality control of animal feeding, especially the possible harm of potential animal viruses to humans, is a key issue that needs to be resolved. With the development of bio...

AI Technical Summary

Problems solved by technology

Nowadays, insect cell expression systems are commonly used to prepare VLPs, but for VLPs composed of multiple proteins, it is necessary to prepare baculoviruses expressing each protein, and then co-infect insect cells with these baculoviruses in different proportions to package the required VLPs. Recombinant baculovirus is essential for expression, and the mixed baculovirus in the product brings certain difficulties to later purification

In addition, the glycosylation of the insect cell expression system is still different from that of mammalian cells, which may affect the later immune effect

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