Light analyzing device evaluation method and phantom sample

Abstract

The purpose of the present invention is to easily and accurately evaluate an optical system of a light analyzing device. Provided is an evaluation method for a light analyzing device, comprising an optical system (A) capable of forming a confocal volume (C) at a focal point by concentrating excitation light (B), that includes: a step of disposing a phantom sample (1) that is made by arranging a plurality of solid members (2, 3), which are of two or more types having different concentrations of a fluorescent material, to be adjacent in the focal point of the optical system (A); a step of moving the confocal volume (C) formed by the optical system (A) relative to the phantom sample (1) in the direction that the solid members (2, 3) are arranged and emitting the excitation light onto the phantom sample (1) via the optical system (A); a step of detecting fluorescent light generated by the solid members (2, 3) disposed in the confocal volume (C); and a step of evaluating the optical system (A) on the basis of the detected fluorescent light.

Description

technical field [0001] The invention relates to an evaluation method of an optical analysis device and a phantom sample. Background technique [0002] Conventionally, an optical analysis device is known (for example, refer to Patent Document 1.) that can quantitatively observe phases that can be processed by optical analysis techniques including statistical processing such as fluorescence correlation spectroscopy (Fluorescence Correlation Spectroscopy: FCS). The state and characteristics of luminescent particles in an aqueous solution with a lower concentration. [0003] This optical analysis device uses an optical system capable of detecting light from a confocal region (confocal region) in an aqueous solution, such as a confocal microscope or a multiphoton excitation microscope. Then, when the position of the confocal region is moved in the aqueous solution, that is, while scanning the aqueous solution through the confocal region, it is detected that light-emitting partic...

AI Technical Summary

Problems solved by technology

[0007] At this time, when it is confirmed that the number of peaks obtained for a certain predetermined concentration is less than the number estimated from the optical system, or the height of the pulse train of photons forming the peak is small, that is, the intensity of fluorescence is small, etc., these Indicates that the state of the optical system is inaccurate

Images