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Extract of potentilla anserina rhizome and medical application thereof

A technology of extracts and rhizomes, which is applied in the field of fernia rhizome extracts and pharmaceutical preparations containing the extracts, can solve the problems of unseen fernia extracts and their use in liver-protecting drugs, and achieve the effect of protecting the liver

Inactive Publication Date: 2015-10-07
HUZHOU PLATEAU BIOLOGICAL RESOURCE IND INNOVATION CENT OF NORTHWEST INST OF PLATEAU BIOLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yang Na et al. explored the extraction process of polysaccharides from Bracken japonica through water extraction, and studied the antioxidant activity of the extract (Yang Na et al., Chinese Journal of Food Science, No. 2, 2014, pp. 60-66). See the research report on the fern extract and its medicinal use in protecting the liver

Method used

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  • Extract of potentilla anserina rhizome and medical application thereof
  • Extract of potentilla anserina rhizome and medical application thereof
  • Extract of potentilla anserina rhizome and medical application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1: preparation of the rhizome extract of Bracken

[0028] Crush 10kg of dried fern rhizomes, extract them under reflux with 75% ethanol (25L×3 times), combine the extracts, concentrate until there is no alcohol smell to obtain a concentrated solution (6L); use petroleum ether (6L×3 times), ethyl acetate (6L×3 times) and water-saturated n-butanol (6L×3 times) to obtain 305g of petroleum ether extract, ethyl acetate extract and n-butanol extract respectively, and n-butanol extraction The substance was dissolved in 5L distilled water and then enriched with AB-8 macroporous resin (2kg, column volume 1.5L). First, 8 column volumes (12L) were washed with 10% ethanol to remove polysaccharides, and then 10 columns were eluted with 65% ethanol. Volume (15L), collect 65% eluate, concentrate under reduced pressure, and spray dry to obtain 185g of Juma rhizome extract extract powder.

Embodiment 2

[0029] Embodiment 2: liquid chromatography analysis

[0030] Preparation of the test solution: Take 5 mg of the extract powder prepared by the method of Example 1 into a 50 mL brown volumetric flask, add 30 mL of 20% acetonitrile aqueous solution for ultrasonic dissolution, and after cooling to room temperature, continue to add 20% acetonitrile aqueous solution to constant volume.

[0031] Analytical method:

[0032] High performance liquid chromatography: Agilent 1260, binary pump;

[0033] Chromatographic column: Diamonsil C18 column (4.6mm×250mm, 5μm);

[0034] Mobile phase: A is acetonitrile, B is 0.1% phosphoric acid solution (adjust the pH value to 6.2 with triethylamine);

[0035] Gradient elution program: 0.01~70min, A 20%→80%;

[0036] Mobile phase flow rate: 1.0mL min -1 ;

[0037] Detection wavelength: 240nm; Column temperature: 30°C;

[0038] Injection volume: 10 μL.

[0039] The extracts prepared from 10 batches of rhizomes from different origins and batche...

Embodiment 3

[0040] Embodiment 3: Pharmacological test of the rhizome extract of Bracken

[0041] 1. Test method

[0042] 1. Cell grouping

[0043]The cells were divided into 6 groups, each with 6 replicate wells: (1) normal culture group (N group): complete culture medium, cultured under normal conditions; (2) ischemia-reperfusion group (IR group): complete culture medium After culturing under normal conditions for 1 hour, simulate hypoxia for 8 hours, and then reperfuse for 4 hours; (3) Bracken extract group: RE1 group: Bracken extract 0.5ng / mL pretreatment for 1 hour; RE2 group: Bracken extract 5ng / mL Pretreatment for 1h; RE3 group: Pretreatment with 50ng / mL Bracken extract for 1h; simulated hypoxia for 8h, reperfusion for 4h; (4) Normal saline group (NS group): pretreatment with the same volume of Bracken extract as normal saline Treat for 1 hour, simulate hypoxia for 8 hours, and reperfuse for 4 hours.

[0044] 2, the pretreatment of fern extract

[0045] (1) Preparation of Bracke...

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Abstract

The invention discloses an extract of potentilla anserina rhizome and medical application thereof. A preparation method for the extract comprises the following steps: (1) crushing dried potentilla anserina rhizome; (b) carrying out heat reflux extraction with 75% ethanol, combining extracting liquid and concentrating the extracting liquid until alcohol smell does not exist so as to obtain concentrate; (c) successively extracting the concentrate obtained in the step (b) with petroleum ether, ethyl acetate and water-saturated n-butanol so as to respectively obtain petroleum ether extract, ethyl acetate extract and n-butanol extract; and (d) enriching the n-butanol extract with macroporous resin, carrying out elution with 10% ethanol weighing 8 times of a column volume so as to remove polysaccharide, then carrying out elution with 65% ethanol weighing 10 times of the column volume, collecting eluate produced by elution with 65% ethanol and subjecting the eluate to pressure-reduced concentration and spray drying. The extract of potentilla anserina rhizome is capable of protecting the liver and can be used for preparing a drug used for protecting the liver.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine, in particular to an extract of the rhizome of Bracken and a pharmaceutical preparation containing the extract. The extract of the rhizome of Bracken can be used to prepare a medicine for protecting the liver. Background technique [0002] The plant fern is a perennial herb, mainly distributed in Qinghai, Gansu, and Tibet. The lower part of the root expands into a spindle-shaped or oval-shaped root tuber, the stem is prostrate, takes root at the node, and often grows a new plant on the ground, and the outer cover is laid down or semi-spreading sparsely pilose or shed and nearly glabrous. The basal leaves are intermittently pinnately compound. Obvious clusters during flowering, 6-11 pairs of leaflets, opposite or alternate, cauline leaves are few or degenerate; petioles are subgrowth or semi-spread sparsely pilose, sometimes fall off nearly glabrous; leaflets are sessile or terminal lea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61P1/16A61K125/00
Inventor 赵晓辉韩发王洪伦
Owner HUZHOU PLATEAU BIOLOGICAL RESOURCE IND INNOVATION CENT OF NORTHWEST INST OF PLATEAU BIOLOGY CHINESE ACADEMY OF SCI
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