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Detection method for rhododendrol in cosmetics

A detection method and rhododendrol technology are applied in the directions of measuring devices, instruments, scientific instruments, etc., which can solve the problems of few detection technologies for rhododendrolol and are in a blank state, and achieve fast and simple sample pretreatment process and sample pretreatment steps. Quick and easy results with fast sample separation

Inactive Publication Date: 2015-10-07
PLANTS & ANIMALS & FOOD TESTING QUARANTINE TECH CENT SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the reports on rhododendol in the literature at home and abroad mainly focus on its occurrence in organisms and its biosynthesis methods, etc., specifically related to the detection technology of rhododendol and the detection technology of rhododendol in cosmetics. blank state

Method used

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  • Detection method for rhododendrol in cosmetics
  • Detection method for rhododendrol in cosmetics
  • Detection method for rhododendrol in cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Condition selection for determination of Rhododendrol in cosmetics by high performance liquid chromatography tandem mass spectrometry (HPLC-MS / MS)

[0035] 1.1 Sample pretreatment: use ethanol as solvent to dissolve rhododendron alcohol. Compared with solvents such as methanol, ethanol is non-toxic and safer.

[0036] 1.2 Mobile phase and elution conditions:

[0037] Methanol-ammonia water was used as the mobile phase, and the gradient elution method was used. Compared with mobile systems such as acetonitrile-water, methanol-water, and acetonitrile-ammonia water, the mobile phase of methanol-ammonia water can realize the separation and detection of rhododendron alcohol in a short time (5 minutes), and the sensitivity is the best.

[0038] 1.3 Selection of mass spectrometry conditions:

[0039] Under the APCI negative ion monitoring mode, rhododendol mainly generates [M-H]-quasimolecular ions, the mass-to-charge ratio (m / z) of the ions is 165, and its reten...

Embodiment 2

[0048] Example 2: Determination of rhododendron in cosmetics by high performance liquid chromatography tandem mass spectrometry (HPLC-MS / MS)

[0049] The reagents and samples used in the experiments were commercially available.

[0050] 1.1 Sample pretreatment: Accurately weigh 1.0g of whitening cosmetic sample (accurate to 0.001g) into a clean 15mL graduated centrifuge tube, dissolve with ethanol, set the volume to 10.0mL scale, and mix well on a vortex shaker , ultrasonic extraction for 10 minutes (if the sample is turbid after extraction, centrifuge at 8000r / min for 10min). Take 2.00mL of the above extract in another clean 15mL graduated centrifuge tube, add methanol-ammonia solution (the volume ratio of methanol to ammonia water is 70:30, ammonia water is 0.05% (V / V) ammonia solution), Dilute to the 10.00mL mark and shake well. Shake the sample and pass it through a 0.22μm needle filter before testing.

[0051] 1.2 Configuration of standard solution:

[0052] (1) Rhodo...

Embodiment 3

[0087] Embodiment 3: Utilize high performance liquid chromatography tandem mass spectrometry (HPLC-MS / MS) to measure the recovery rate and precision test of rhododendron alcohol

[0088] Adding different amounts of standard solutions to the blank cosmetics was used to conduct spike recovery and precision experiments.

[0089] With reference to the test method of Example 2, rhododendol with a concentration of 0.1, 0.5, 1.0, and 2.0 mg / kg was added to the milky cosmetics and the aqueous solution respectively, and six parallel tests were carried out. and aqueous solution as the background, and determine the recovery rate and relative standard deviation according to the test results. The results are shown in Table 4 and Table 5:

[0090] Table 4 Statistical Table of Emulsion Cosmetics Added Standard Recycling

[0091]

[0092] Table 5 Aqueous Solution Cosmetics Spiked Recovery Statistical Table

[0093]

[0094] According to the results of Table 4 and Table 5, the recover...

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Abstract

The invention provides a detection method for rhododendrol in cosmetics. The method comprises the following steps: firstly, carrying out pre-treatment on a sample, dissolving a to-be-detected sample with ethanol, mixing evenly, carrying out ultrasonic extraction, filtering with a filter membrane and then obtaining a sample extract liquid; preparing a series of rhododendrol standard solutions, and determining through a computer on basis of a high-performance liquid chromatography-tandem mass spectrometry, and obtaining a content-result standard curve of the standard solution according to the determination result; and detecting the sample extract liquid through the computer employing the high-performance liquid chromatography-tandem mass spectrometry or an ultra-performance liquid chromatography, and obtaining the content of the rhododendrol in the sample according to the standard curve. By virtue of the scheme of the HPLC-MS / MS (high-performance liquid chromatography-tandem mass / mass spectrometry), the operation method is simple; the sensitivity is high; the accuracy and the stability of the test result are good; and rapid detection on the sample can also be realized by UPLC (ultra-performance liquid chromatography) detection.

Description

technical field [0001] The invention relates to the field of cosmetic detection, in particular to a detection method for rhododendron in cosmetics. Background technique [0002] Rhododendron, the chemical name is 4-(4-hydroxyphenyl)-2-butanol, and its molecular formula is C 10 h 14 o 2 , the molecular weight is 166.2, the pure product is white powder, soluble in water, soluble in organic solvents such as methanol, ethanol and acetonitrile. Most of the active ingredients in modern whitening cosmetics come from natural plant extracts, such as arbutin, Chinese herbal extracts, and rhododendron alcohol, among which rhododendron alcohol is widely distributed in white birch and Japanese maple, and can effectively inhibit the formation of melanin. However, it is precisely because rhododendol can inhibit the synthesis of melanin, which interferes with the normal enzymatic conversion process of tyrosine to melanin in the skin. Whitening cosmetics added with rhododendron will cause...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 杨振宇倪昕路汪侃晨陈章庭
Owner PLANTS & ANIMALS & FOOD TESTING QUARANTINE TECH CENT SHANGHAI ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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