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A method and application for signal immobilization detection of nanomaterial colorimetric sensing system based on chromatography paper-integrating sphere

A colorimetric sensing and nanomaterial technology, applied in the field of signal immobilization detection of nanomaterial colorimetric sensing system, can solve problems such as the influence of time error test results, and achieve the effect of reducing errors

Active Publication Date: 2017-11-17
HEFEI NORMAL UNIV
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Problems solved by technology

Therefore, in addition to strict requirements on the time control of each test (the test must be carried out at the moment when the nanoparticles are aggregated for a certain time), this type of detection method will have time errors caused by the operation of the experimenter and the scanning of the solution by the instrument. affect the test results

Method used

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  • A method and application for signal immobilization detection of nanomaterial colorimetric sensing system based on chromatography paper-integrating sphere
  • A method and application for signal immobilization detection of nanomaterial colorimetric sensing system based on chromatography paper-integrating sphere

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Embodiment 1

[0019] Embodiment 1: the detection of mercury ion

[0020] The mercury ion recognition DNA sequence (1 μM, 10 μL) was mixed with different concentrations of mercury solutions (10 μL), left to stand for 30 minutes, and the nano-gold solution (10 nM, 100 μL) was added. After 15 minutes, NaCl solution (420 mM, 10 μL) was added (the above steps are consistent with the conventional colorimetric sensing method based on gold nanoparticles).

[0021] After 3 minutes (obtained by the step of establishing the pre-termination time), take 50 μL of the mixed solution and drop it on the chromatography paper to form regular circular spots. On the integrating sphere attachment of the spectrophotometer, align the area to be measured with the light spot to collect the absorption spectrum. Carry out the quantitative analysis of target substance by data processing at last, the result that utilizes this inventive method to obtain to mercury response is as follows figure 1 shown.

[0022] pass ...

Embodiment 2

[0023] Example 2: Application of pH Alarm System

[0024] The A15 DNA sequence (0.5 μM, 10 μL) was mixed with buffers of different pH values, allowed to stand for 30 minutes, and a nano-gold solution (10 nM, 100 μL) was added. After 15 minutes, NaCl solution (420 mM, 10 μL) was added (the above steps are consistent with the conventional colorimetric sensing method based on gold nanoparticles).

[0025] After 3 minutes (obtained by the step of establishing the pre-termination time), take 50 μL of the mixed solution and drop it on the chromatography paper to form regular circular spots. On the integrating sphere attachment of the spectrophotometer, align the area to be measured with the light spot to collect the absorption spectrum. Carry out the quantitative analysis of target substance by data processing at last, the result that utilizes this inventive method to obtain to mercury response is as follows figure 2 shown.

[0026] pass figure 2 In the middle photo (above), y...

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Abstract

A method and application for signal immobilization detection of a nanomaterial colorimetric sensing system based on a chromatography paper-integrating sphere, relating to the field of nanomaterial-based colorimetric detection methods, a traditional nanomaterial colorimetric sensing system and a target After the interaction and color development, take an appropriate amount of the mixed dispersion droplets on the chromatography paper at the pre-termination time to form regular circular spots. After natural drying, use the chromatography paper as a blank, and place the sample in a UV-visible spectrophotometer. On the attachment of the integrating sphere, the area to be measured is aligned with the light spot to collect the absorption spectrum, and finally the quantitative analysis of the target is carried out through data processing. The invention adopts the method of combining chromatographic paper and integrating sphere, which not only fixes the signal of the nanomaterial in dynamic aggregation, but also overcomes the disadvantage that the conventional paper analysis technology is difficult to accurately measure. At the same time, the determination is not limited by time, and the obtained results are more reliable and accurate.

Description

technical field [0001] The invention relates to the field of colorimetric detection methods based on nanomaterials, in particular to a method and application of signal immobilization detection for nanomaterial colorimetric sensing systems based on chromatography paper-integrating spheres. Background technique [0002] Colorimetry is a commonly used method in detection, because of its excellent surface plasmon resonance (SPR), many metal nanomaterials, such as gold nanoparticles, gold nanorods, nanosilver, etc. It presents different colors and is widely used as a colorimetric probe for the detection of environmental, biological and other analytes. Nanomaterials such as carbon nanotubes and graphene oxide have also been used for the colorimetric detection of analytes based on the color transition produced by particle aggregation. [0003] Since the colorimetric-based detection is performed in aqueous solution, the analytical operation has great limitations. To facilitate det...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/78
Inventor 郑斌程盛董华泽朱金苗丁运生刘健
Owner HEFEI NORMAL UNIV
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