Manufacturing method for artificial atractylis lancea seed

A technology of artificial seeds and production methods, applied in the fields of biotechnology and agricultural engineering, can solve the problems of reduced yield and exhaustion of Atractylodes atractylodes germplasm resources, and achieve low cost, reduced transplanting and domestication process, and convenient storage and transportation Effect

Inactive Publication Date: 2015-10-21
JIANGSU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Zhenjiang, Jiangsu Province is the main production area of ​​Cangzhu, and the surrounding Jintan, Jurong, Lishui, Liyang, Gaochun and other places have also been the raw material suppliers of Cangzhu since the 1950s, but after decades of development Since the 1980s, its output has been declining, and in recent years, the wild germplasm resources of Atractylodes atractylodes are almost exhausted, although in order to meet the growing market demand, relevant departments have begun to guide the development of Atractylodes atractylodes However, due to the many requirements for the growth of Atractylodes atractylodes and the limitations of various technical conditions, it is far from meeting the needs of the market. Therefore, it is also a local agricultural production and planting requirement for economic development to realize the increase in production of Atractylodes atractylodes through feasible technical means. One of the important and long-term tasks of quality resource conservation!

Method used

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  • Manufacturing method for artificial atractylis lancea seed
  • Manufacturing method for artificial atractylis lancea seed
  • Manufacturing method for artificial atractylis lancea seed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Adjust the hydrosol coating material and proportion in the embedding solution, and carry out the germination experiment of the artificial seeds of Atractylodes atractylodes.

[0033] The embedding solution, that is, the ratio of the coating material mixed with sodium alginate and gelatin hydrosol, is adjusted, and the artificial seeds of Atractylodes atractylodes are produced according to the steps described in the summary of the invention, and the coated explants are single buds or protocorms. Observe the germination and rooting of the artificial seeds of Atractylodes atractylodes, and the physiological indexes of the artificial seeds of Atractylodes atractylodes in different ratios of seed coat coating materials are shown in Table 1.

[0034] Table 1 Physiological indicators of artificial seeds of Atractylodes atractylodis in drip-buried with different seed coat coating materials

[0035]

[0036] As can be seen from Table 1, the main physiological indicators of th...

Embodiment 2

[0038] Adjust the endosperm nutrient adsorbent in the embedding solution, and carry out the germination experiment of the artificial seeds of Atractylodes atractylodes.

[0039] Adding endosperm nutrient adsorbent can absorb the nutrition of artificial seeds and prevent leakage. The endosperm nutrient adsorbent in the embedding solution is adjusted, and the artificial seeds of Atractylodes atractylodes are produced according to the steps described in the summary of the invention. The coated explants are single buds, and the germination and rooting of the artificial seeds of Atractylodes atractylodes are observed, and different results are obtained. Table 2 shows the effects of different types of endosperm nutrient adsorbents on the growth of Atractylodes atractylodes artificial seeds.

[0040] Table 2 Effects of different types of endosperm nutrient adsorbents on the growth of artificial seeds of Atractylodes atractylodes

[0041] Adsorbent type

[0042] It can be see...

Embodiment 3

[0044] Adjust the hormone compatibility in the embedding solution, and observe the rooting effect of the artificial seeds of Atractylodes atractylodes.

[0045] Adjust the hormone compatibility in the embedding solution, make the artificial seeds of Atractylodes atractylodes according to the steps described in the summary of the invention, and the coated explants are protocorms, observe the rooting situation of the artificial seeds of Atractylodes atractylodes, and obtain a comparison of different hormones. The results of the rooting effect of Atractylodes atractylodes artificial seeds are shown in Table 3.

[0046] Table 3 Effects of different hormone ratios on the rooting of Atractylodes atractylodes artificial seeds

[0047]

[0048]

[0049] As can be seen from the results in Table 3, in a single NAA treatment group, the rooting rate is the highest at the level of 0.7mg / LNAA, and the root system grows the most vigorously, and the effect is the best, and the NAA at th...

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Abstract

The invention discloses a manufacturing method for an artificial atractylis lancea seed. The method mainly comprises an optimum selection scheme of related action conditions like acquisition of an explant material, selection of an episperm coating material and compatibility of an endosperm adsorbent, a carbon source, culture medium concentration and hormone for manufacturing an artificial seed. The artificial seed manufactured by using the method in the invention can get rid of restrictions of season, temperature and humidity environments on the processes like generation, formation, budding, germination and the like of a natural atractylis lancea seed, and can produce a genuine traditional Chinese medicinal raw material of atractylis lancea through large-scale industrial propagation and breeding under the technical conditions of tissue culture and asexual propagation.

Description

technical field [0001] The invention belongs to the fields of biotechnology and agricultural engineering, and in particular relates to a method for making artificial seeds of atractylodes atractylodes. Background technique [0002] Mao Cangzhu, also known as Southern Cangzhu, is a perennial herb of the genus Atractylodes in the family Asteraceae. It likes temperature, coolness, cold resistance and drought resistance. It mostly grows in loose sandy and loamy soils with good air permeability on hills and hillsides. It is mainly distributed in East China, the Yangtze River Basin, In the central and southern regions, Anhui, Jiangsu, Sichuan, and Hubei are more common. Among them, Maoshan in Zhenjiang, Jiangsu is recognized as the center of production of authentic Mao Cangzhu medicinal materials, so it is called "Mao Cangzhu" because of Maoshan. Daodi Mao Atractylodes is a precious Chinese medicinal material, which can promote liver protein synthesis, anti-virus effects, prevent ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 吴沿友乔韡轶吴沿胜邢德科谷睿智赵玉国王金田刘彩
Owner JIANGSU UNIV
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