Composition, method, kit and use thereof for preparing pluripotent stem cells
A pluripotent stem cell and stem cell technology, which is applied in the field of efficient and stable preparation of compositions for highly immunodeficient mammalian pluripotent stem cells, and can solve problems such as obstacles
Active Publication Date: 2018-08-17
PEKING UNIV +1
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Problems solved by technology
The above shortcomings may hinder its further application to the establishment of humanized mouse models and other fields
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[0037] The experimental methods used in the following examples are conventional methods unless otherwise specified.
[0038] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.
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Abstract
The invention provides a composite for preparing the pluripotent stem cells of a mammal with high immunodeficiency, such as a mouse, in a high efficient and stable mode, a method of applying the composite, a reagent box containing the composite and the application thereof. The composite comprises stem cell basic culture media and vitamin C or vitamin C ramification.
Description
technical field [0001] The invention provides a composition, method, kit and application for efficiently and stably preparing pluripotent stem cells of highly immunodeficiency mammals (such as mice). Background technique [0002] In order to improve the understanding and knowledge of human hematopoiesis, innate immunity, infectious diseases, cancer and regenerative medicine, the stem cell field has been committed to the development of humanized mouse models (Bente et al., 2005; King et al., 2008 ; Kumar et al., 2008; Sun et al., 2007; Yajima et al., 2009). In recent years, many important experimental advances have been made in the development of humanized mice, one of which is the establishment of highly immunodeficient NOD-scidIl2rg – / – (NPG) mouse model, which has the characteristics of severe combined immunodeficiency (SCID) and interleukin-2Rg (Il-2Rg) allele loss (Ito et al., 2002). The genetic background of non-obese diabetic immunodeficiency in NPG mice leads to imp...
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IPC IPC(8): C12N5/0735C12N5/071C12N15/873A01K67/027
Inventor 邓宏魁刘康方日国李海波苗振川其他发明人请求不公开姓名
Owner PEKING UNIV
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