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A microbial compound bacterial agent and method for improving the survival rate of afforestation in abandoned coal gangue land

A compound inoculant, wasteland technology, applied in microorganism-based methods, biochemical equipment and methods, and botanical equipment and methods, etc. problems, to achieve the effect of promoting rapid vegetation restoration, good application prospects, and improving the survival rate of afforestation

Active Publication Date: 2018-10-02
山西辰谦集团有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The regolith soil of coal gangue hills has a large bulk density and small porosity, which makes it have poor water holding capacity and low effective water content compared with normal soil, which directly affects the normal growth of plants.
At the same time, the strong acid environment is conducive to the release of heavy metals and other substances in coal gangue, which will also affect the survival and growth of plants.
Coal gangue piles are piled up by gangue, lack of animal and microbial activities, which delays the ripening speed of surface coal gangue and weathered matter, is not conducive to the improvement of its physical structure, and restricts the growth and development of plants
Therefore, in the ecological restoration process of coal gangue wasteland, it faces harsh conditions such as extreme lack of plant nutrition, high temperature, water shortage, acid damage, salt damage and heavy metal stress, which makes the survival rate of afforestation low

Method used

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  • A microbial compound bacterial agent and method for improving the survival rate of afforestation in abandoned coal gangue land

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The microbial composite microbial agent for improving the survival rate of afforestation in the coal gangue wasteland of this embodiment is prepared by the following method: 1×10 9 CFU / ml Bacillus mucilaginosus Krassilnikov GIM1.15 bacterial solution, 2×10 9 CFU / ml Trichoderma harzianum Rifai GIM3.442 bacterial liquid, 3×10 9 CFU / ml Rhodobacter azotoformans CGMCC 1.5023 bacterial solution, 2×10 8 CFU / ml Pseudomonas fluorescens Migula GIM1.209 bacterial solution, 8×10 8 CFU / ml Bacillus megatherium GIM1.270 bacterial liquid is mixed according to the volume ratio of 5:1:0.5:2:4 to form a mixed bacterial liquid. In addition, the modified starch and rice husk charcoal are mixed according to the volume ratio of 1:9, As the bacterial liquid adsorbent, the mixed bacterial liquid and the bacterial liquid adsorbent are then inoculated on the mixture of modified starch and rice husk charcoal in a volume ratio of 1:10, stirred evenly, and spray dried to obtain improved coal gangue Mic...

Embodiment 2

[0030] The microbial composite microbial agent for improving the afforestation survival rate of coal gangue wasteland of this embodiment is prepared by the following method: 5×10 9 CFU / ml Bacillus mucilaginosus Krassilnikov GIM1.15 bacterial solution, 6×10 8 CFU / ml Trichoderma harzianum Rifai GIM3.442 bacterial liquid, 5×10 9 CFU / ml Rhodobacter azotoformans CGMCC 1.5023 bacterial liquid, 5×10 8 CFU / ml Pseudomonas fluorescens Migula GIM1.209 bacterial solution, 8×10 8 CFU / ml Bacillus megatherium GIM1.270 bacterial liquid is mixed according to the volume ratio of 1.5:0.8:1:1:1.5 to form a mixed bacterial liquid. In addition, the modified starch and rice husk charcoal are mixed according to the volume ratio of 1:3, As the bacterial liquid adsorbent, the mixed bacterial liquid and the bacterial liquid adsorbent are then inoculated on the mixture of modified starch and rice husk charcoal at a volume ratio of 1:20, stirred evenly, and spray dried to obtain improved coal gangue Microbia...

Embodiment 3

[0032] The microbial composite microbial agent for improving the afforestation survival rate of coal gangue wasteland of this embodiment is prepared by the following method: 8×10 9 CFU / ml Bacillus mucilaginosus Krassilnikov GIM1.15 bacterial solution, 3×10 9 CFU / ml Trichoderma harzianum Rifai GIM3.442 bacterial liquid, 9×10 9 CFU / ml Rhodobacter azotoformans CGMCC 1.5023 bacterial liquid, 9×10 8 CFU / ml Pseudomonas fluorescens Migula GIM1.209 bacterial liquid, 6×10 9 CFU / ml Bacillus megatherium GIM1.270 bacterial liquid is mixed according to the volume ratio of 6:2:3:3:5 to form a mixed bacterial liquid. In addition, the modified starch and rice husk charcoal are mixed according to the volume ratio of 1:5, As the bacterial liquid adsorbent, the mixed bacterial liquid and the bacterial liquid adsorbent are then inoculated on the mixture of modified starch and rice husk charcoal in a volume ratio of 2:10, stirred evenly, and spray dried to obtain improved coal gangue Microbial compou...

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Abstract

The present invention discloses a microbial compound bacterium agent and method for improving coal gangue wasting land afforestation survival rate. 1 * 109-8 * 109CFU / ml of gum Bacillus bacteria GIM1.15 bacterial liquid, 6 * 108-3 * 109CFU / ml of Trichoderma harzianum GIM3.442 bacterial liquid, 3 * 109-9 * 109CFU / ml of nitrogen-fixing Rhodospirillum photometricum CGMCC 1.5023 bacterial liquid, 2 * 108-9 * 108CFU / ml of Pseudomonas fluorescens GIM1.209 bacterial liquid and 8 * 108-6 * 109CFU / ml of huge Bacillus bacteria GIM1.270 bacterial liquid are mixed in volume ratio of 1.5- 6: 0.8- 2: 0. 5-3:1-3:1. 5-5 to form a mixed bacterial liquid; then modified starch and rice husk charcoal are mixed in volume ratio of 1-5:9 to be use as a bacterial liquid adsorbent, then the mixed bacterial liquid is inoculated onto the bacterial liquid adsorbent in the volume ratio of 5-20: 100 of the mixed bacterial liquid to the bacterial liquid adsorbent; and the microbial compound bacterium agent can be obtained by even stirring and spray-drying. The microbial compound bacterium agent can significantly improve the coal gangue wasting land afforestation survival rate, thus promoting rapid revegetation of a coal gangue wasting land, and the microbial compound bacterium agent has good prospects in the field of ecological restoration of the coal gangue wasting land.

Description

Technical field: [0001] The invention belongs to the technical field of ecological restoration of abandoned mines, and specifically relates to a microbial composite microbial agent and a method for improving the survival rate of afforestation in coal gangue abandoned land. Background technique: [0002] A large amount of coal gangue is produced in the process of coal mining. Generally, about 10%-30% of coal gangue is associated with every 1 ton of coal mined. Coal gangue has become one of the largest industrial waste residues in my country. The accumulation of coal gangue not only takes up a lot of land, but also pollutes the surrounding environment, such as soil and vegetation pollution. The soil bulk density of the weathered layer of coal gangue hills is relatively large, and the porosity is relatively small, which makes the water holding capacity poor and low effective water content compared with normal soil, which directly affects the normal growth of plants. At the same tim...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/14A01N63/04A01P21/00A01G7/06A01G23/00C12R1/07C12R1/885C12R1/39C12R1/11C12R1/01
CPCY02A40/22Y02A40/28Y02P60/40
Inventor 王小东黄小春刘丽婷王丽艳黄建建叶金山李田
Owner 山西辰谦集团有限公司